[1]谭黎霞,田强强,王敦,等. 海棠花离体繁殖技术研究[J].西北林学院学报,2012,27(02):93-97.
　TAN Li-xia,TIAN Qiang-qiang,WANG Dun,et al. Micropropagation of Malus spectabilis in vitro[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(02):93-97.
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 海棠花离体繁殖技术研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第27卷
期数:
2012年02期
页码:
93-97
栏目:
出版日期:
2012-03-30

文章信息/Info

Title:
 Micropropagation of Malus spectabilis in vitro
文章编号:
1001-7461(2012)02-0093-05
作者:
 谭黎霞; 田强强; 王敦; 阙怡; 李厚华
 （1.西北农林科技大学 植物保护学院,陕西 杨陵 712100；2.西北农林科技大学 林学院,陕西 杨陵 712100）
Author(s):
 TAN Li-xia;  TIAN Qiang-qiang;  WANG Dun;  QUE Yi;  LI Hou-hua
 (1.College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100,China; 2. College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China)
关键词:
 ; 海棠花; 叶片; 不定芽再生; 组织培养
Keywords:
 ; Malus spectabilis;  leaf;  adventitious shoot regeneration;  tissue culture
分类号:
S685.043
文献标志码:
A
摘要:
 以海棠花组培苗和叶片为材料，应用正交试验法，以MS 培养基为基础，分别加入不同浓度配比的6BA/IBA 组合和TDZ/IBA 组合作为增殖培养基和叶盘不定芽再生培养基，研究了不同植物激素及其配比对组培苗增殖、叶片不定芽再生的影响。结果表明：在添加6BA 1.0 mg·L-1 + IBA 0.1 mg·L-1的MS 培养基上最适宜海棠花组培苗的增殖及生长，增殖倍数高达8.11，海棠花组培苗平均高度达2.14 cm；在添加TDZ 1.0 mg·L-1+ IBA 0.5 mg·L-1的MS 培养基上叶再生效率最高，再生效率为100%，每叶平均再生芽数为6.73。

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