[1]陈少瑜,张雨,陈霞,等. 27份核桃种质资源亲缘关系的ISSR分析[J].西北林学院学报,2012,27(04):108-112.
 CHEN Shao-yu,ZHANG Yu,CHEN Xia,et al. Genetic Relationship of 27 Walnut Germplasm Resources Based on ISSR Markers[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(04):108-112.
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 27份核桃种质资源亲缘关系的ISSR分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第27卷
期数:
2012年04期
页码:
108-112
栏目:
出版日期:
2012-07-30

文章信息/Info

Title:
 Genetic Relationship of 27 Walnut Germplasm Resources Based on ISSR Markers
文章编号:
1001-7461(2012)04-0108-05
作者:
 陈少瑜张雨陈霞毛云玲董润全
 (1.云南省林业科学院 云南省森林植物培育与开发利用重点实验室,云南 昆明 650204;2.云南省林业科学院 经济林研究所,云南 昆明 650204;3.云南省农业科学院 园艺研究所,云南 昆明 650205)
Author(s):
 CHEN Shao-yu ZHANG Yu CHEN Xia MAO Yun-ling DONG Run-quan
 (1.The Key Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry, Kunming, Yunnan 650204, China; 2. Institute of Economic Forest Trees, Yunnan Academy of Forestry, Kunming, Yunnan 650204, China; 3. Horticulture Institute, Yunnan Academy of Agriculture Science, Kunming, Yunnan 650205, China)
关键词:
 核桃种质资源ISSR分子标记亲缘关系
Keywords:
 walnut germplasm resources ISSR molecular marker genetic relationship
分类号:
S664.1
文献标志码:
A
摘要:
 采用ISSR分子标记技术对4个种的27份核桃种质进行亲缘关系分析。从120条ISSR引物中筛选出扩增条带清晰稳定、重复性好的10条引物对27份核桃种质基因组DNA进行扩增,共扩增出129条带,其中91条呈现多态性,多态性条带的比例平均为70.54%。不同核桃种质间遗传相似性系数在0.496 1~1.000 0之间。研究结果表明:27份核桃种质具有较为丰富的遗传多样性,铁核桃与核桃的亲缘关系较近(最近),与野核桃的亲缘关系最远。基于遗传相似性系数的UPGMA聚类结果表明,27份核桃种质按照种的划分被明显地分成4大组,与传统形态分类一致。该结果为核桃种质资源的进一步研究和利用提供参考。

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