[1]赵杨,李玉璞,代毅. 华山松SRAP-PCR反应体系的优化[J].西北林学院学报,2012,27(05):87-90.
 ZHAO Yang,LI Yu-pu,DAI Yi. Optimization of SRAP-PCR System for Pinus armandii [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(05):87-90.
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 华山松SRAP-PCR反应体系的优化()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第27卷
期数:
2012年05期
页码:
87-90
栏目:
出版日期:
2012-09-20

文章信息/Info

Title:
 Optimization of SRAP-PCR System for Pinus armandii
文章编号:
1001-7461(2012)05-0087-04
作者:
 赵杨李玉璞代毅
 (1.贵州大学 林学院,贵州 贵阳 550225;2.平坝国有林场,贵州 平坝 561100)
Author(s):
 ZHAO Yang LI Yu-pu DAI Yi
 (1. College of Forestry, Guizhou University, Guiyang, Guizhou 550225, China;2. Pingba State Forest Farm, Pingba, Guizhou 561100, China)
关键词:
 华山松 SRAP 反应体系
Keywords:
 Pinus armandii SRAP reaction system
分类号:
S791.241.04
文献标志码:
A
摘要:
 为建立华山松SRAP-PCR反应体系,研究先采用L16(45)正交设计对影响华山松SRAP反应的5个因子(DNA模板浓度、Mg2+浓度、dNTPs浓度、引物浓度、Taq酶)在4个水平上进行优化试验。结果表明:各因素对SRAP反应的影响依次为:Mg2+>dNTPs>Taq 酶>DNA 模板>引物;对SRAP反应结果影响较大的3个因子(Mg2+浓度、dNTPs浓度、Taq 酶浓度)进行单因素试验;确立华山松SRAP反应最佳体系为:20 μL的PCR体系中含有Mg2+ 2.2 mmol/L、dNTPs 0.25 mmol/L、DNA模板60 ng、Taq酶0.8 mol/s、引物0.8 μmol/L。将该体系用于华山松的SRAP扩增能获得稳定、清晰的多态性条带。

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