[1]金会鑫,谢寿安,史睿杰,等. 不同方法提取蚱蝉若虫蛋白质效果比较[J].西北林学院学报,2012,27(06):128-130.
　JIN Hui-xin,XIE Shou-an,SHI Rui-jie,et al. Comparison of Chemical Extraction Methods of the Proteins from the Larva of Cryptotympana pustulata[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(06):128-130.
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 不同方法提取蚱蝉若虫蛋白质效果比较()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第27卷
期数:
2012年06期
页码:
128-130
栏目:
出版日期:
2012-11-30

文章信息/Info

Title:
 Comparison of Chemical Extraction Methods of the Proteins from the Larva of Cryptotympana pustulata
文章编号:
1001-7461(2012)06-0128-03
作者:
 金会鑫; 谢寿安; 史睿杰; 汤耀宗; 王翻艳
 （西北农林科技大学 林学院，陕西 杨陵 712100）
Author(s):
 JIN Hui-xin;  XIE Shou-an;  SHI Rui-jie;  TANG Yao-zong;  WANG Fan-yan
 (College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China)
关键词:
 ; 蚱蝉若虫; 蛋白质提取方法; 正交试验
Keywords:
 ; Cryptotympana pustulatav larva;  proteins;  orthogonal method;  extraction methods
分类号:
S759.8
文献标志码:
A
摘要:
 以蚱蝉（Cryptotympana pustulata）若虫为材料，采用正交试验法，对蚱蝉若虫蛋白质的提取条件进行了研究。结果表明，碱提蛋白法的蛋白质得率最高，其次为盐提蛋白法，Tris-HCl提蛋白法得率最低。碱提蛋白法的主要工艺指标为：碱液质量分数0.5%、温度60℃、时间60 min、固液体积比为1∶5时蛋白质得率有最大值；盐提蛋白法的主要工艺指标为：盐液质量分数1.5%、温度40℃、时间120 min、固液体积比为1∶10时蛋白质得率有最大值；Tris-HCl提蛋白法的主要工艺指标为：Tris-HCl液pH值8.1、抽提温度20℃、抽提时间30 min、固液体积比1∶10时蛋白质得率有最大值。

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