[1]陈祖静,费昭雪,曹支敏,等. 杨树与叶锈菌互作中的细胞程序性死亡[J].西北林学院学报,2013,28(03):134-137.[doi:10.3969/j.issn.1001-7461.2013.03.26]
 CHEN Zu-jing,FEI Zhao-xue,CAO Zhi-min,et al. Programmed Cell Death in the Interaction between Poplar and Leaf Rust[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(03):134-137.[doi:10.3969/j.issn.1001-7461.2013.03.26]
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 杨树与叶锈菌互作中的细胞程序性死亡()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第28卷
期数:
2013年03期
页码:
134-137
栏目:
出版日期:
2013-05-30

文章信息/Info

Title:
 Programmed Cell Death in the Interaction between Poplar and Leaf Rust
文章编号:
1001-7461(2013)03-0134-04
作者:
 陈祖静费昭雪曹支敏白露超        
 (西北农林科技大学 林学院,陕西 杨陵 712100)
Author(s):
 CHEN Zu-jing FEI Zhao-xue CAO Zhi-min BAI Lu-chao
 (College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China)
关键词:
 杨树落叶松-杨栅锈菌过敏性坏死反应细胞程序性死亡
Keywords:
poplar Melampsora larici-populina hypersensitive response programmed cell death
分类号:
S718.523
DOI:
10.3969/j.issn.1001-7461.2013.03.26
文献标志码:
A
摘要:
 用落叶松-杨栅锈菌(Melampsora larici-populina)菌系Gl052和Sb052分别接种抗病川杨(Populus szechuanica)与感病太白杨(P.purdomii)叶片,对叶肉细胞核进行荧光染色观察及基因组DNA Ladder条带检测,初步探讨杨树与锈菌互作过程中的寄主细胞程序性死亡进程。结果表明:抗病品种川杨受落叶松-杨栅锈菌侵染后有过敏性坏死现象,DNA Ladder检测发现有降解的DNA小片段,染色观察发现接种0.5 dpi细胞核开始发生变化,3~4 dpi细胞核裂解,7 dpi细胞核消失,表现出明显的细胞程序性死亡特征,在DNA电泳图谱上观察到明显的梯状DNA条带。感病品种太白杨受该叶锈菌侵染后未检测到降解的DNA小片段,荧光染色观察细胞核也未明显变化,未明显表现出PCD特征。
Abstract:
 Disease resistant variety,Populus szechuanica and susceptible variety P.purdomii were inoculated with Melampsora larici-populina isolates Gl052 and Sb052,respectively to investigate the process of programmed cell death (PCD) of the host cells during the interaction between poplar trees and rust fungi by the observation of fluorescence staining of mesophyll nucleus and detection of DNA ladder band.Hypersensitive necrosis occurred on the disease resistant variety P.szechuanica.The nucleus began to change at 0.5 dpi (days post inoculation),cracked at 3 to 4 dpi,and disappeared at 7 dpi,which represents typical features of PCD.Meanwhile,the DNA ladder bands which display the DNA degradation were observed.For the susceptible variety,P.purdomii,no obvious features of PCD were observed (no small degradated DNA fragments were detected,and no obvious changes occurred in the mesophyll nuclei after staining).

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备注/Memo

备注/Memo:
 收稿日期:2012-07-09  修回日期:2012-09-27
基金项目:国家自然科学基金(30872027)。
作者简介:陈祖静,女,在读博士,研究方向:森林病理学。
*通信作者:曹支敏,男,教授,博士生导师,主要研究方向:森林病理与菌物学。E-mail: zmcao@nwsuaf.edu.cn
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