[1]曹弈璘,曾春菡,刘小波,等. PEG模拟干旱胁迫下4种苔藓植物的生理指标变化及其耐旱性评价[J].西北林学院学报,2014,29(04):33-39.[doi:doi:10.3969/j.issn.1001-7461.2014.04.06]
　CAO Yi-lin,ZENG Chun-han,LIU Xiao-bo,et al. Physiological and Biochemical Response and Drought Resistance Evaluation of Four Wild Bryophytes[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(04):33-39.[doi:doi:10.3969/j.issn.1001-7461.2014.04.06]
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 PEG模拟干旱胁迫下4种苔藓植物的生理指标变化及其耐旱性评价()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年04期
页码:
33-39
栏目:
出版日期:
2014-07-30

文章信息/Info

Title:
 Physiological and Biochemical Response and Drought Resistance Evaluation of Four Wild Bryophytes
文章编号:
1001-7461(2014)04-0033-07
作者:
 曹弈璘; 曾春菡; 刘小波; 王丽*
 （四川大学 生命科学学院，四川 成都 610065）
Author(s):
 CAO Yi-lin;  ZENG Chun-han;  LIU Xiao-bo;  WANG Li*
 （College of Life Sciences, Sichuan University, Chengdu, Sichuan 610065, China）
关键词:
 ; 苔藓植物; 干旱胁迫; 耐旱性; 生理指标
Keywords:
 ; bryophyte;  drought stress;  drought tolerance;  physiological index
分类号:
S718.3
DOI:
doi:10.3969/j.issn.1001-7461.2014.04.06
文献标志码:
A
摘要:
 以4种常见苔藓植物（狭叶小羽藓、尖叶美喙藓、柔叶青藓和鳞叶藓）为试验材料，用15%PEG进行胁迫30 h，再复水24 h的模拟干旱胁迫试验。对干旱胁迫过程中４种植物的叶绿素、游离脯氨酸、丙二醛、可溶性蛋白、细胞质膜透性、超氧化物歧化酶、过氧化物酶和过氧化氢酶等生理生化指标进行测定，结合形态观察对４种植物的耐旱性进行评价。结果表明，在干旱胁迫下４种植物的叶绿素、丙二醛含量、细胞质膜透性及3种酶活性随时间增加呈现下降趋势，脯氨酸含量表现为上升到缓慢上升的过程，可溶性蛋白含量除柔叶青藓以外的3种苔藓都呈现先上升后下降的趋势。在复水24 h后。狭叶小羽藓和鳞叶藓的叶绿素含量有明显的上升,但另2种仍有略微下降。且4种苔藓的丙二醛和细胞质膜透性均表现为下降趋势。综合分析表明，狭叶小羽藓在各项指标中均显示为耐旱性较强的品种，其次为尖叶美喙藓和鳞叶藓，两者耐旱能力相差不大，柔叶青藓为耐旱性较差的品种。
Abstract:
 In order to provide the theoretical and practical references for selecting and applying drought tolerance plantscape resources, four common bryophytes (Haplocladium angustifolium, Eurhynchium eustegium, Brachythecium moriense, Taxiphyllum taxirameum) were treated by 15% PEG for drought-stress test. Physiological and biochemical indexes（contents of chlorophyll, free proline, MDA, soluble proteins, cell membrane permeability, SOD, POD, and CAT）were measured and compared, combined with morphological observations, the drought resistances of 4 species were evaluated. The results showed the contents of chlorophyll and MDA, cell membrane permeability and the activities of three kinds of enzymes presented downward trends with time. The content of free proline showed a slow process of rising, and that of soluble protein increased first and then decreased. After 24 h rehydration, the contents of MDA and cell membrane permeability of 4 species still decreased. The contents of chlorophyll of H. angustifolium and T. taxirameum significantly increased, while that of the other species appeared slightly downward trends. According to the comprehensive analysis of all indicators, it was suggested that H. angustifolium exhibited the strongest tolerance to drought, while B. moriense was the weakest. The difference of drought tolerance between E.eustegium and T.taxirameum was not obvious.

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备注/Memo

备注/Memo:
 收稿日期：2013-10-18修回日期：2013-11-22
基金项目：国家自然科学基金项目（2008FY110400-2-1）。
作者简介：曹弈璘，女，硕士研究生，研究方向：园林植物。E-mail:caoyilin1125@163.com
*通信作者：王丽，女，博士研究生，研究方向：园林植物。E-mail: biolyw@gmail.com
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