[1]蒋弘刚,魏安智*,杨途熙,等. 花椒茎尖节点转录组测序及基因注释[J].西北林学院学报,2014,29(06):94-99.[doi:doi:10.3969/j.issn.1001-7461.2014.06.18]
 JIANG Hong-gang,WEI An-zhi*,YANG Tu-xi,et al. Transcriptome Sequencing of Node Tissue of Stem Tip of Zanthoxylum bungeanum and Gene Annotation[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(06):94-99.[doi:doi:10.3969/j.issn.1001-7461.2014.06.18]
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 花椒茎尖节点转录组测序及基因注释()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年06期
页码:
94-99
栏目:
出版日期:
2014-11-30

文章信息/Info

Title:
 Transcriptome Sequencing of Node Tissue of Stem Tip of Zanthoxylum bungeanum and Gene Annotation
文章编号:
1001-7461(2014)06-0094-06
作者:
 蒋弘刚1魏安智1*杨途熙1代惠萍2
 (1 西北农林科技大学 林学院,陕西 杨陵 712100; 2 陕西理工学院,陕西 汉中 723000)
Author(s):
 JIANG Hong-gang1WEI An-zhi1*YANG Tu-xi1DAI Hui-ping2
 (1. College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China)
关键词:
 花椒皮刺分化转录组高通量测序
Keywords:
Zanthoxylum bungeanum prickle differentiation transcriptome high-throughput sequencing
分类号:
S722.39
DOI:
doi:10.3969/j.issn.1001-7461.2014.06.18
文献标志码:
A
摘要:
 花椒茎枝有尖锐皮刺,在实际生产中增加采摘成本,并降低采摘效率。为培育品质优良的无刺品种,筛选皮刺分化的关键基因具有重要意义。使用二代高通量测序技术对花椒皮刺起始分化的茎尖节点组织转录组进行测序,使用生物学软件对原始数据进行拼装,最终获得45 057条转录本序列。使用比对工具将得到的转录本与Swiss-Prot,NR,Pfam,KEGG,KOG数据库比对后,80.34%的转录本序列至少被一个数据库注释。获取的大量的基因序列及注释信息为进一步探究花椒皮刺分化分子机理提供了良好的基础,为定位皮刺分化关键基因提供依据。
Abstract:
 The fruit of Zanthoxylum bungeanum is one of the most popular spices in China. However, there are numerous short prickles on both the stems and the branches, which result high cost and low efficiency of picking the fruit. Therefore, breeding of spine free cultivar is needed. During this process, localization of essential genes in prickle differentiation is above all. We sequenced the node of stem where prickle differentiation initiation by Next-generation sequencing technology. After de novo assembly, we obtained 45 057 transcripts of the node of the stem. In addition, we annotated the transcriptome against five public database (Swiss-Prot, NR, Pfam, KEGG and KOG). The massive sequence and annotation information would build a solid base for non-prickle hua-jiao breeding.

参考文献/References:

 [1]中国科学院中国植物志编辑委员会.中国植物志(43卷2分册)[M].北京: 科学出版社, 1997.
[2]KARIN L,ANDERS ,LAETITIA L, et al. Developmental regulation of indole-3-acetic acid turnover in Scots pine seedlings[J]. Plant Physiology, 2001, 125(1): 464-475.
[3]KALIKA P,PANKAJ D. Polar auxin transport: cell polarity to patterning[M]// CHEN R J, BALU K F. Polar auxin transport. Springer Berlin Heidelberg, 2013:25-44.
[4]BENKOVA E, MICHNIEWICZ M, SAUER M, et al. Local, efflux-dependent auxin gradients as a common module for plant organ formation[J]. Cell, 2003, 115(5): 591-602.
[5]BHALERAO R P, BENNETT M J. The case for morphogens in plants[J]. Nat Cell Biol., 2003, 5(11): 939-43.
[6]ERLICH Y, MITRA P P, MCCOMBIE W R, et al. Alta-cyclic: a self-optimizing base caller for next-generation sequencing[J]. Nature Methods, 2008, 5(8): 679-682.
[7]GRABHERR M G., HAAS B J, YASSOUR M, et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome[J]. Nature Biotechnology, 2011, 29(7): 644-652.
[8]PERTEA G, HUANG X, LIANG F, et al. TIGR gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets[J]. Bioinformatics, 2003, 19(5): 651-652.
[9]VERNOUX T, BRUNOUD G, FARCOT E, et al. The auxin signalling network translates dynamic input into robust patterning at the shoot apex[J]. Mol. Syst. Biol., 2011, 7: 508.
[10]BIANCO M D, KEPINSKI S. Context, specificity, and self-organization in auxin response[J]. Cold Spring Harbor Perspectives Biol., 2011, 3(1): 1-20.
[11]BOWMAN J L. The YABBY gene family and abaxial cell fate[J]. Curr. Opin. Plant Biol., 2000, 3(1): 17-22.
[12]BONACCORSO O, LEE J E, PUAH L, et al. Filamentous flower controls lateral organ development by acting as both an activator and a repressor[J]. BMC Plant Biol., 2012, 12: 176.
[13]SAWA S, ITO T, SHIMURA Y, et al. Filamentous flower controls the formation and development of arabidopsis inflorescences and floral meristems[J]. Plant Cell, 1999, 11(1): 69-86.
[14]王港,李周岐,刘晓敏,等. 花椒组织培养再生体系的建立[J]. 西北林学院学报, 2008, 23(3): 117-119.
WANG G,LI Z Q,LIU X M,et al. Establishment of tissue culture regeneration system of Zanthoxylum bungeanum[J]. Journal of Northwest Forestry University, 2008, 23(3): 117-119.(in Chinese)
[15]薛惠丹,李周岐. 花椒愈伤组织 EMS 诱变及变异研究[J]. 西北林学院学报, 2012, 27(2): 98-101.
XUE H D,LI Z Q. Variation and EMS mutagenesis in callus of Zanthoxylum bungeanum[J]. Journal of Northwest Forestry University, 2012, 27(2): 98-101. (in Chinese)

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备注/Memo

备注/Memo:
 收稿日期:2014-05-23修回日期:2014-07-10
基金项目:国家林业局林业公益性行业科研专项(201304706)。
作者简介:蒋弘刚,男,在读硕士,研究方向:林木遗传改良。E-mail: freshair@vip.qq.com
*通信作者:魏安智,男,教授,研究方向:林木遗传改良。E-mail: weianzhi@126.com
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