[1]屈子睿,马养民*,许倩,等. 防风内生真菌DL02菌株次生代谢产物的结构与活性[J].西北林学院学报,2015,30(06):217-221.[doi:doi:10.3969/j.issn.1001-7461.2015.06.39]
　QU Zi-rui,MA Yang-min*,XU Qian,et al. Structure and Activity of Secondary Metabolites of Endophytic Fungus DL02 Strain in Saposhnikovia divaricate[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(06):217-221.[doi:doi:10.3969/j.issn.1001-7461.2015.06.39]
点击复制

 防风内生真菌DL02菌株次生代谢产物的结构与活性()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年06期
页码:
217-221
栏目:
出版日期:
2015-11-30

文章信息/Info

Title:
 Structure and Activity of Secondary Metabolites of Endophytic Fungus DL02 Strain in Saposhnikovia divaricate
文章编号:
1001-7461(2015)06-0217-05
作者:
 屈子睿; 马养民*; 许倩; 闫梦茹; 王健; 苗智
 （陕西科技大学 化学与化工学院 教育部轻化工助剂化学与技术重点实验室，陕西 西安 710021）
Author(s):
 QU Zi-rui;  MA Yang-min*;  XU Qian;  YAN Meng-ru;  WANG Jian;  MIAO Zhi
 (Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education, College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi’an, Shaanxi 710021,China)
关键词:
 ; 防风; 内生真菌; 裂褶菌; 次生代谢产物; 抑菌活性
Keywords:
Saposhnikovia divaricate;  endophytic fungus;  Schizophyllum commune;  secondary metabolite;  antibacterial activity
分类号:
S713
DOI:
doi:10.3969/j.issn.1001-7461.2015.06.39
文献标志码:
A
摘要:
 为探究防风内生真菌DL02次生代谢产物的结构与活性，采用形态学特征和ITS序列鉴定菌株，利用硅胶柱层析、Sephadex LH-20柱层析和重结晶等方法对该菌株的发酵产物进行分离和纯化，并结合所得化合物的理化性质和波谱数据鉴定其结构，通过最小抑菌浓度（MIC）对其次生代谢产物进行活性测试。结果表明，菌株DL02为裂褶菌（Schizophyllum commune Franch.），从其发酵产物中分离得到7个化合物，分别为烟酸（1）、丁二酸（2）、麦角甾醇（3）、22E-5α，8α-桥二氧麦角甾-6，22-二烯-3β-醇（4）、色胺酮（5）、lawsaritol A（6）、胡萝卜苷（7），其中化合物6为首次从真菌发酵产物中分离得到，化合物5有很好的抑菌活性，其中对大肠杆菌的MIC值为3.9 μg·mL-1。
Abstract:
 The fermentation products of endophytic fungus DL02 strain in Saposhnikovia divaricate identified by morphological feature and ITS sequence were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography and recrystallization methods. Their structures were identified according to physicochemical property and spectrum data, and an MIC method was used in antibacterial activity test. The results showed that DL02 strain was identified as Schizophyllum commune Franch., and seven compounds were identified as nicotinic acid (1), succinic acid (2), ergosterol (3), 22E-5α,8α-epidioxyergosta-6,22-dien-3β-ol (4), tryptanthrin (5), lawsaritol A (6) and daucosterol (7). Compound 6 was reported as a metabolite from fungi for the first time. Compound 5 showed inhibitory activity with a MIC value of 3.9 μg·mL-1 to Escherichia coli.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(06):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(06):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(06):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(06):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(06):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(06):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(06):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(06):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(06):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(06):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]

备注/Memo

备注/Memo:
 收稿日期：2015-04-27 修回日期：2015-06-03
基金项目：高等学校博士学科点专项科研基金（20116125110001）；陕西省自然科学基金（2014JZ003）。
作者简介：屈子睿，女，在读硕士，研究方向：植物内生真菌。E-mail：qurongrong16@163.com
*通信作者：马养民，男，教授，博士生导师，研究方向：植物内生真菌。E-mail：mym63@sina.com
更新日期/Last Update: