[1]马玉花,冶贵生,马娟,等. 柴达木盆地梭梭P5CS基因的扩增及序列分析[J].西北林学院学报,2016,31(1):88-91.[doi:10.3969/j.issn.1001-7461.2016.01.16]
 MA Yu-hua,YE Gui-sheng,MA Juan,et al. Amplication and Sequence Analysis of P5CS Gene in Haloxylon ammodendron in Qaidam Basin[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(1):88-91.[doi:10.3969/j.issn.1001-7461.2016.01.16]
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 柴达木盆地梭梭P5CS基因的扩增及序列分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第1期
页码:
88-91
栏目:
出版日期:
2016-01-31

文章信息/Info

Title:
 Amplication and Sequence Analysis of P5CS Gene in Haloxylon ammodendron in Qaidam Basin
文章编号:
1001-7461(2016)01-0088-04
作者:
 马玉花冶贵生马娟冶桂莲龙晓晨李玉英马秀芳
 (青海大学 农牧学院,青海 西宁 810016)
Author(s):
 MA Yu-huaYE Gui-shengMA JuanYE Gui-lianLONG Xiao-chenLI Yu-yingMA Xiu-fang
 (College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai 810016,China)
关键词:
 梭梭P5CS基因扩增序列分析
Keywords:
Haloxylon ammodendronP5CS gene amplification sequence analysis
分类号:
S727.23
DOI:
10.3969/j.issn.1001-7461.2016.01.16
文献标志码:
A
摘要:
以柴达木盆地梭梭同化枝为材料,扩增其P5CS基因并进行序列测定。结果表明:所得柴达木盆地梭梭P5CS基因的碱基数量为442个,编码146个氨基酸。另外,同源性比对分析结果显示梭梭与甜菜、可可等植物的P5CS基因具有较高的同源性。研究结果将为下一步梭梭P5CS基因的结构与功能研究奠定基础。
Abstract:
 The fragment of P5CS gene of assimilating branches of Haloxylon ammodendron in Qaidam Basin was amplified and sequenced by the specific primers,then the nucleotide sequence and amino acid sequence of P5CS gene was identified and summarized.The sequence analysis indicated that the total length of P5CS geng was 442 bp to encode 146AA.Homology analysis showed that there had high homology among Haloxylon ammodendron P5CS gene and other plants.This results would lay foundation for the further study of the structure of P5CS gene and it’s function in H.ammodendron.

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备注/Memo

备注/Memo:
 收稿日期:2015-01-21修回日期:2015-03-24
基金项目:青海省科技厅自然科学基金项目(2012-Z-938Q)。
作者简介:马玉花,女,博士,副教授,研究方向:森林培育理论与技术、植物资源开发利用。E-mail:sophere8@163.com
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