[1]马玉花,冶贵生,冯志鹏. 柴达木盆地梭梭CMO基因的克隆及其蛋白结构预测[J].西北林学院学报,2016,31(2):115-119.[doi:10.3969/j.issn.1001-7461.2016.02.20]
 MA Yu-hua,YE Gui-sheng,FENG Zhi-peng. Gene Analysis,Protein Prediction of CMO of Haloxylon ammodendron in Qaidam Basin[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(2):115-119.[doi:10.3969/j.issn.1001-7461.2016.02.20]
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 柴达木盆地梭梭CMO基因的克隆及其蛋白结构预测()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第2期
页码:
115-119
栏目:
出版日期:
2016-03-31

文章信息/Info

Title:
 Gene Analysis,Protein Prediction of CMO of Haloxylon ammodendron in Qaidam Basin
文章编号:
1001-7461(2016)02-0115-05
作者:
 马玉花冶贵生冯志鹏
 (青海大学 农牧学院,青海 西宁 810016)
Author(s):
 MA Yu-huaYE Gui-shengFENG Zhi-peng
 (Institute of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai 810016,China)
关键词:
 梭梭CMO基因序列分析蛋白结构
Keywords:
Haloxylon ammodendron CMO gene sequence analysis protein structure
分类号:
S718.4
DOI:
10.3969/j.issn.1001-7461.2016.02.20
文献标志码:
A
摘要:
 对柴达木盆地梭梭的CMO基因进行扩增,并利用生物软件对CMO基因序列进行分析、对蛋白结构进行预测。结果表明,柴达木盆地梭梭CMO基因长度为1 341 bp,编码447个氨基酸。CMO蛋白亲水性、二级结构、亚细胞定位、信号肽、跨膜螺旋区、三级结构预测结果显示柴达木盆地梭梭CMO蛋白亚细胞定位于叶绿体,无信号肽,蛋白二级结构主要为无规则卷曲。CMO蛋白具有多个多种类型的功能位点,为蛋白功能的实现提供了保障。此外CMO蛋白的亲水性较强,无跨膜螺旋区。柴达木盆地梭梭CMO蛋白三维结构预测结果显示CMO蛋白三维结构由α螺旋、β折叠和无规则卷曲互相盘绕而成。
Abstract:
 CMO gene of Haloxylon ammodendron ocurring in Qaidam Basin was amplified by PCR and it’s protein structure was predicted by biology software.The results showed that the length of the CMO gene and CMO protein were 1 677 bp and 559 aa respectively.The prediction results of hydrophilicity,secondary structure,subcellular localization,signal peptide,transbilayer helix and tertiary structure showed that CMO protein was located in chloroplast,without signal peptide,random coils were the main secondary structure of CMO protein and it had many kinds of functional sites.CMO protein had good hydrophilic property,and it had no transmembrane structures.The tertiary structure prediction showed the main tertiary structure of CMO protein included α-helix,β fold and random coil.

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备注/Memo

备注/Memo:
 收稿日期:2015-04-24修回日期:2015-07-20
基金项目:青海省科技厅自然科学基金项目(2012-Z-938Q);“123高层次人才培养工程”项目。
作者简介:马玉花,女,回族,博士,副教授,研究方向:森林培育理论与技术、植物资源开发利用。E-mail:sophere8@163.com
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