[1]粟莉圆,李大培,常远,等. 核桃JrGSTTau1基因的克隆及转入酵母的抗逆功能分析[J].西北林学院学报,2016,31(3):98-102.[doi:10.3969/j.issn.1001-7461.2016.03.16]
　SU Li-yuan,LI Da-pei,CHANG Yuan,et al.Cloning and Stress Tolerance Analysis of a JrGSTTau1 Gene from Juglans regia in Saccharomyces cerevisiae[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(3):98-102.[doi:10.3969/j.issn.1001-7461.2016.03.16]
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 核桃JrGSTTau1基因的克隆及转入酵母的抗逆功能分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第3期
页码:
98-102
栏目:
出版日期:
2016-05-31

文章信息/Info

Title:
Cloning and Stress Tolerance Analysis of a JrGSTTau1 Gene from Juglans regia in Saccharomyces cerevisiae
文章编号:
1001-7461(2016)03-0098-05
作者:
 粟莉圆; 李大培; 常远; 卢烨彤; 康桐铭; 杨桂燕*
 (西北农林科技大学 林学院 核桃研究中心,陕西 杨陵 712100）
Author(s):
 SU Li-yuan; LI Da-pei; CHANG Yuan; LU Ye-tong; KANG Tong-ming; YANG Gui-yan*
 （Laboratory of Walnut Research Center,College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China）
关键词:
 ; 核桃; 谷胱甘肽转移酶; 酵母表达系统; 逆境响应
Keywords:
Juglans regia;  glutathione S-transferase;  yeast expression system;  stress response
分类号:
S718.46
DOI:
10.3969/j.issn.1001-7461.2016.03.16
文献标志码:
A
摘要:
 为筛选核桃（Juglans regia）抗逆相关的功能候选基因，研究克隆获得一条核桃Tau亚家族GST基因（JrGSTTau1），并将该基因插入酵母表达载体pYES2中构建重组酵母pYES2-JrGSTTau1，将重组载体转入酿酒酵母（Saccharomyces cerevisiae）INVSC1，同时以转化空载体pYES2的重组酵母作为阴性对照。在酵母表达系统中研究该基因的抗逆功能。结果表明，JrGSTTau1的开放读码框（ORF）全长690 bp，拟推导的蛋白分子量为26.856 kDa，含有氨基酸数为229，理论等电点为6.02。对2种酵母进行NaCl、CdCl2、-20℃和53℃胁迫处理，比较发现JrGSTTau1转基因酵母表现出较对照更高的生命力和成活率。表明JrGSTTau1基因能有效提高酵母的耐盐抗镉及抵抗异常温度的能力，JrGSTTau1可作为核桃逆境应答的候选基因。
Abstract:
 To screen the stress tolerance related genes in Juglans regia,in current study,a Tau sub-family GST gene was cloned (Named as JrGSTTau1) and JrGSTTau1 was inserted into the yeast expression vector pYES2 for constructing recombinant plasmid pYES2-JrGSTTau1,which was transformed into the Saccharomyces cerevisiae strain INVSC1.The yeast transformed with empty pYES2 was used as a negative control.The yeast expression system was used for studying of the abiotic stress tolerance.The results indicated that the full length open reading frame (ORF) of JrGSTTau1 was 690 bp,the deduced protein was 26.856 kD with 229 amino acids,and the theoretical pI was 6.02.When both recombinant yeast were treated with NaCl,CdCl2,-20℃ and 53℃,the JrGSTTau1 expressed yeast displayed higher vitality and survival rate than control yeast under four stress conditions.It was concluded that JrGSTTau1 gene could effectively improve the salt,cadmium,cold and heat tolerance of transgenic yeast,JrGSTTau1 may be an important candidate gene for J.regia exposed to adverse stimulus.

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备注/Memo

备注/Memo:
 收稿日期：2015-10-03修回日期：2016-04-18
基金项目：陕西省农业科技创新与攻关项目(2015NY122)；西北农林科技大学博士科研启动基金（2014BSJJ038）；西北农林科技大学本科生创新训练项目（1201510712045）；西北农林科技大学基本科研业务费专项资金项目（2452015171）。
作者简介：粟莉圆，女，侗族，本科，专业：林学。E-mail:826701143@qq.com
*通信作者：杨桂燕，女，苗族，讲师，博士，研究方向：林木遗传育种。E-mail:yangguiyan@nwsuaf.edu.cn
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