[1]沈慧,黄建,佟岩,等. 枣基因组大小研究[J].西北林学院学报,2016,31(3):138-142.[doi:10.3969/j.issn.1001-7461.2016.03.23]
　SHEN Hui,HUANG Jian,TONG Yan,et al. Comparative Analysis of Genome Sizes of Chinese Jujube[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(3):138-142.[doi:10.3969/j.issn.1001-7461.2016.03.23]
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 枣基因组大小研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第3期
页码:
138-142
栏目:
出版日期:
2016-05-31

文章信息/Info

Title:
 Comparative Analysis of Genome Sizes of Chinese Jujube
文章编号:
1001-7461(2016)03-0138-05
作者:
 沈慧1; 黄建1; 佟岩2; 李新岗1*
 （1.西北农林科技大学 林学院，国家枣工程技术研究中心，陕西 杨陵 712100；2.中国科学院 昆明植物研究所，中国西南野生生物种质资源库，云南 昆明 650204）
Author(s):
 SHEN Hui1; HUANG Jian1; TONG Yan2; LI Xin-gang1*
 (1.College of Forestry,Northwest A&F University,Research Centre for Jujube Engineering and Technology of State Forestry Administration,Yangling,Shaanxi 712100,China; 2.Plant Germplasm and Genomics Center,Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan 650204,China)
关键词:
 ; 枣; 酸枣; 流式细胞术; 基因组大小; 比较分析
Keywords:
 ; jujube (Ziziphus jujuba);  wild jujube (Z.jujuba var.spinosa);  flow cytometry
分类号:
S718.43
DOI:
10.3969/j.issn.1001-7461.2016.03.23
文献标志码:
A
摘要:
 采用流式细胞技术，以4个酸枣类型、9个枣品种的新鲜幼嫩叶片为材料，以Otto为解离液，日本晴（Oryza sativa ssp.japonica cv.Nipponbare）为外标，测定其基因组大小（2C值），比较枣和酸枣的2C值差异。结果表明，枣的基因组大小为(360.70±4.73)Mb，小于酸枣的基因组（388.20±8.32 Mb）；枣品种间基因组大小无显著差异，酸枣类型间差异显著，说明酸枣具有更高的基因组变异，且酸枣到枣的进化中可能伴随着基因组的缩减。
Abstract:
 The genome sizes (2C-values) of 4 wild jujube species (Ziziphus jujuba var.spinosa) and 9 jujube varieties (Ziziphus jujuba) were tested by using flow cytometry technique.The fresh young leaves were used as materials,Otto (Oryza sativa L.ssp.japonica cv.Nipponbare) was used as the external reference.The genome size varieties were compared between jujube and wild jujube.The average genome size of jujube was estimated to be (360.70±4.73)Mb,which was smaller than the average genome size of wild jujube (388.20±8.32 Mb).There existed significant differences between the types of wild jujube in genome size but no significant differences between jujube varieties.The results indicated that the genome size variability of wild jujube was higher than jujube and the domestication may accompany with genome size reduction from wild jujube to jujube.

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备注/Memo

备注/Memo:
 收稿日期：2015-07-22修回日期：2015-12-01
基金项目：国家“十二五”科技支撑计划课题（2013BAD14B03）；陕西省科技统筹课题（2013KTZB02-03）。
作者简介：沈慧，女，在读硕士，研究方向：经济林栽培。E-mail:saries@ nwsuaf.edu.cn
*通信作者：李新岗，男，教授，博士生导师，研究方向：经济林栽培。E-mail:xingangle@nwsuaf.edu.cn
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