[1]陈熙,张羽*,李佼,等. SRAP标记分析陕西省主要茶树种质资源遗传多样性[J].西北林学院学报,2016,31(3):143-147.[doi:10.3969/j.issn.1001-7461.2016.03.24]
　CHEN Xi,ZHANG Yu*,LI Jiao,et al. Analysis of SRAP Marker-based Polymorphism of the Main Tea Germplasms in Shaanxi[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(3):143-147.[doi:10.3969/j.issn.1001-7461.2016.03.24]
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 SRAP标记分析陕西省主要茶树种质资源遗传多样性()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第3期
页码:
143-147
栏目:
出版日期:
2016-05-31

文章信息/Info

Title:
 Analysis of SRAP Marker-based Polymorphism of the Main Tea Germplasms in Shaanxi
文章编号:
1001-7461(2016)03-0143-06
作者:
 陈熙1; 张羽1*; 李佼2; 李秀峰2; 席彦军2
（1.陕西理工学院 生物科学与工程学院，陕西 汉中 723000；2.汉中市农业科学研究所，陕西 汉中 723000）
Author(s):
 CHEN Xi1; ZHANG Yu1*; LI Jiao2; LI Xiu-feng2; XI Yan-jun2
 (1.School of Biological Sciences and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723000,China; 2.Hanzhong Institute of Agricultural Sciences,Hanzhong,Shaanxi 723000,China)
关键词:
 ; 茶树; SRAP; 多样性
Keywords:
 ; tea;  SRAP;  polymorphism
分类号:
S718.46
DOI:
10.3969/j.issn.1001-7461.2016.03.24
文献标志码:
A
摘要:
 用6份有代表性的陕西茶树资源材料，从154对SRAP引物组合中筛选出40 对扩增条带清晰、多态性高的引物组合，对陕西省50个茶树资源进行遗传多样性研究。结果表明，SRAP标记能够揭示材料间较高的遗传多样性，共检测到336个等位基因，每对引物组合可检测到5~13个等位位点，平均为8个；每个SRAP位点的遗传多态性信息含量在0.78~1.00之间，平均值为0.93。供试材料的遗传相似系数集中在0.73~0.95之间。可见，基于SRAP标记的陕西省主要茶树资源遗传多样性处于较高水平。
Abstract:
 Genetic diversity of 50 tea germplasms in Shaanxi was investigated by using variable renaturing temperature-based PCR.Taking 6 representative tea plants as templates,40 pairs of SRAP markers were screened out from total 154 pairs of primers,which showed specific and obvious PCR products.The results demonstrated that total 336 alleles were verified using SRAP markers,and about 5-13 alleles were detected by each pair of molecular markers with an average of 8 alleles.The genetic polymorphism information contents of each SRAP locus were between 0.78-1.00,with an average of 0.93.Additionally,the genetic similarities of the 50 varieties were between 0.73-0.95.Based on SRAP markers,the genetic diversity of these tea materials was relatively high.

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备注/Memo

备注/Memo:
 收稿日期：2015-07-20修回日期：2015-10-20
基金项目：陕西省自然科学基础研究计划项目（2013JZ008）；陕西省科技统筹创新工程项目（2013KTZB02-01-01）。
作者简介：陈熙，女，在读硕士，研究方向：植物分子生物学。E-mail:326906112@qq.com
*通信作者：张羽,女,教授，研究方向：植物分子生物学。E-mail: zy68169@sina.com
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