[1]崔丹,魏安智*,侯娜,等. 花椒离体愈伤组织诱导研究[J].西北林学院学报,2016,31(5):138-141.[doi:10.3969/j.issn.1001-7461.2016.05.23]
 CUI Dan,WEI An-zhi*,HOU Na,et al.In vitro Callus Induction of Zanthoxyhum bungeanum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(5):138-141.[doi:10.3969/j.issn.1001-7461.2016.05.23]
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 花椒离体愈伤组织诱导研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第5期
页码:
138-141
栏目:
出版日期:
2016-09-30

文章信息/Info

Title:
In vitro Callus Induction of Zanthoxyhum bungeanum
文章编号:
1001-7461(2016)05-0138-04
作者:
 崔丹魏安智*侯娜刘玉林
 (西北农林科技大学 林学院,陕西 杨陵 712100)
Author(s):
 CUI DanWEI An-zhi*HOU NaLIU Yu-lin
 (College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 花椒愈伤组织诱导分化
Keywords:
Zanthoxyhum bungeanum callus induced differentiation
分类号:
S722.3
DOI:
10.3969/j.issn.1001-7461.2016.05.23
文献标志码:
A
摘要:
 以“凤县大红袍”花椒嫩叶为外植体,研究不同种类和浓度的植物生长调节剂、不同盐浓度的培养基以及同一低温不同处理时间对花椒愈伤组织诱导及其生长的影响。结果表明,MS为最适培养基;诱导花椒愈伤组织较优的生长调节剂组合为,0.5 mg/L 6-BA+0.5 mg/L 2,4-D,诱导出的愈伤生长旺盛,质地松散,无褐化现象;在0℃处理2、4 h仍可以获得正常的愈伤组织,不影响再分化。
Abstract:
 The tender leaves of “Fengxian Dahongpao”,a cutivar of Zanthoxyhum bungeanum were used as explants to study the effect of different factors on callus induction and the growth of Zanthoxyhum bungeanum.The results showed that MS was the best medium.The preferable combination of growth regulators was 0.5 mg/L 6-BA+0.5 mg/L 2,4-D,in which the calli grew vigorously with loose texture,and without browning.Furthermore,normal callus could still be obtained when treated 2 and 4 h in 0℃,indicating low temperature treatment did not affect re-differentiation.

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备注/Memo

备注/Memo:
 收稿日期:2015-11-11修回日期:2016-02-25
基金项目:秦巴山区重要林药泛素化筛选与规范化栽培关键技术研究(201204603);国家林业局林业公益性行业科研专项项目“花椒良种选育及丰产栽培技术研究”(201304706)。
作者简介:崔丹,女,在读硕士,研究方向:林木遗传育种。E-mail:253822170@qq.com
*通信作者:魏安智,男,教授,研究方向:林木遗传育种。E-mail:weianzhi@126.com
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