[1]张弘弛,刘瑞*,李慧,等. 黄芪内生真菌AR15中吲哚二酮哌嗪及其对黄芪白粉病原菌的抑制作用[J].西北林学院学报,2017,32(1):197-202.[doi:10.3969/j.issn.1001-7461.2017.01.31]
 ZHANG Hong-chi,LIU Rui*,LI Hui,et al. Indole Diketopiperazines from Endophyic Fungus AR15 of Astragalus membranaceus and Antimicrobial Activity for Erysiphe pisi[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(1):197-202.[doi:10.3969/j.issn.1001-7461.2017.01.31]
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 黄芪内生真菌AR15中吲哚二酮哌嗪及其对黄芪白粉病原菌的抑制作用()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第1期
页码:
197-202
栏目:
出版日期:
2017-01-31

文章信息/Info

Title:
 Indole Diketopiperazines from Endophyic Fungus AR15 of Astragalus membranaceus and Antimicrobial Activity for Erysiphe pisi
文章编号:
1001-7461(2017)01-0197-06
作者:
 张弘弛12刘瑞12*李慧12安志鹏12周凤12
 (1.山西大同大学 生命科学学院,山西 大同 037009;2.山西大同大学 应用生物技术研究所,山西 大同 037009)
Author(s):
 ZHANG Hong-chi12LIU Rui12*LI Hui12AN Zhi-peng12ZHOU Feng12
 (1.College of Life Science,Shanxi Datong University,Datong,Shanxi 037009,China; 2.Applied Biotechnology Institute,Shanxi Datong University,Datong,Shanxi 037009,China)
关键词:
 内生真菌黄芪吲哚二酮哌嗪分离黄芪白粉病
Keywords:
 endophytic fungus Astragalus membranaceus indole diketopiperazine isolation Erysiphe pisi
分类号:
S567.2
DOI:
10.3969/j.issn.1001-7461.2017.01.31
文献标志码:
A
摘要:
 为了研究黄芪内生真菌AR15的次级代谢产物的结构及其对黄芪白粉病原菌的抑制作用。采用形态学方法和ITS序列鉴定该AR15,使用反复硅胶柱色谱法、Sephadex LH-20凝胶色谱法,结合HPLC对AR15的发酵产物进行分离纯化,通过波谱分析鉴定其发酵液中分离得到8个化合物的结构。结果表明:黄芪内生真菌AR15为Penicillium sp.,分离到的8个吲哚二酮哌嗪类生物碱分别鉴定为fumitremorgin B(1)、cyclotryprostatins B(2)、verruculogen(3)、tryprostatin B(4)、tryprostatin A(5)、cyclotryprostatin A(6)、cyclotryprostatins C(7)、cyclotryprostatins D(8),所有吲哚二酮哌嗪对黄芪白粉病原菌都显示出一定的抑制作用,其中化合物1,3和6的MIC值达到3.13、1.56和6.25 μg·mL-1
Abstract:
 The chemical constituents from endophytic fungus AR15 of Astragalus membranaceus and antimicrobial activity to Erysiphe pisi were investigated.According to the morphological features,cultural characteristics and ITS sequences,the strains AR15 were indentified as Penicillium sp.The compounds were isolated by column chromatography,Sephadex LH-20,and HPLC.Eight indole diketopiperazines were obtained and elucidated as fumitremorgin B (1),cyclotryprostatins B (2),verruculogen (3),tryprostatin B (4),tryprostatin A (5),cyclotryprostatin A (6),cyclotryprostatins C (7),and cyclotryprostatins D (8).All indole diketopiperazines showed inhibitory activity to E.pisi,and compound 1,3 and 6 showed strong inhibitory activity with MIC values of 3.13,1.56 and 6.25μg·mL-1,respectively.

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备注/Memo

备注/Memo:
 收稿日期:2016-05-14修回日期:2016-06-23
基金项目:山西省科技攻关项目(20130311011-6);大同市科技攻关项目(201468-1) 。
作者简介:张弘弛,男,博士,研究方向:微生物次生代谢产物。E-mail:zhanghclw@163.com
*通信作者:刘瑞,女,博士,研究方向:天然产物。E-mail:zhanghclw@163.com
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