[1]藕丹,樊军锋*,周永学,等. 美洲黑杨×青杨派杂种无性系SSR指纹图谱的构建与遗传差异性分析[J].西北林学院学报,2017,32(2):112-116.[doi:10.3969/j.issn.1001-7461.2017.02.19]
 OU Dan,FAN Jun-feng*,ZHOU Yong-xue,et al. Construction of Fingerprint and Analysis of Genetic Difference for Hybrid Clones of Populus deltoides×Section tacamahaca Using SSR Marker[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(2):112-116.[doi:10.3969/j.issn.1001-7461.2017.02.19]
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 美洲黑杨×青杨派杂种无性系SSR指纹图谱的构建与遗传差异性分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第2期
页码:
112-116
栏目:
出版日期:
2017-03-31

文章信息/Info

Title:
 Construction of Fingerprint and Analysis of Genetic Difference for Hybrid Clones of Populus deltoides×Section tacamahaca Using SSR Marker
文章编号:
1001-7461(2017)02-0112-05
作者:
 藕丹樊军锋*周永学高建社
 (西北农林科技大学 林学院,陕西 杨陵 712100)
Author(s):
 OU DanFAN Jun-feng*ZHOU Yong-xueGAO Jian-she
 (College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 杨树无性系分子标记SSR指纹图谱
Keywords:
 Populus clone molecular marker SSR fingerprint
分类号:
S792.110
DOI:
10.3969/j.issn.1001-7461.2017.02.19
文献标志码:
A
摘要:
 用SSR分子标记构建9个美洲黑杨×青杨派杂种无性系和3个父母本的指纹图谱并进行遗传差异性分析。研究表明,所筛选出的12对引物共扩增出94条清晰条带,每对引物平均扩增条带为7.8条,其中,多态性条带85条,多态性比率达到90.4%;各杨树无性系间遗传相似系数在0.44~0.80之间,平均相似系数为0.62,并利用多态性条带构建出12个杨树无性系的分子指纹图谱。通过系统聚类分析发现,杂种无性系与母本美洲黑杨遗传差异性小,且在该指纹图谱中,每个无性系的谱带都不同,说明不同供试无性系在DNA分子水平上表现出一定的遗传差异,据此可将它们区别开来。
Abstract:
 DNA fingerprints of 9 hybrid clones of Populus deltoides×Section tacamahaca and 3 parents were established by using SSR (simple sequence repeats) marker,and differences in genetic relationships were analyzed.The results showed that 94 clear bands were generated from 12 pairs of primers selected and each primer amplified 7.8 bands,in which the polymorphic bands were 88 and the polymorphic rate was 90.4%.The similarity coefficients of the Populus clones ranged from 0.44 to 0.80 with an average of 0.62.Those polymorphic bands were used for establishing fingerprint of 12 clones.The clustering analysis showed that the genetic differences between those hybrid clones and female parent (P.deltoides) were low.Each clone had its own unique bands,which showed that different experimental materials had certain genetic differences in DNA level ,which could differentiate them.

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备注/Memo

备注/Memo:
 收稿日期:2016-06-21修回日期:2016-11-21
基金项目:国家林业局林业公益性行业科研专项(201404113)。
作者简介:藕丹,女,在读硕士,研究方向:林木遗传育种林业生物技术。E-mail:18829784191@163.com
*通信作者:樊军锋,男,博士,教授,研究方向:杨树新品种选育及油松遗传改良。E-mail:fanjf28@163.com
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