[1]刘宝尧,刘小利*,魏海斌,等. 青海地方核桃种质资源遗传多样性研究[J].西北林学院学报,2017,32(2):130-135.[doi:10.3969/j.issn.1001-7461.2017.02.22]
 LIU Bao-yao,LIU Xiao-li*,WEI Hai-bin,et al. Genetic Diversity of Germplasm Resources of Walnut in Qinghai[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(2):130-135.[doi:10.3969/j.issn.1001-7461.2017.02.22]
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 青海地方核桃种质资源遗传多样性研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第2期
页码:
130-135
栏目:
出版日期:
2017-03-31

文章信息/Info

Title:
 Genetic Diversity of Germplasm Resources of Walnut in Qinghai
文章编号:
1001-7461(2017)02-0130-06
作者:
 刘宝尧12刘小利2*魏海斌2顾文毅2
 (1.青海大学 农牧学院,青海 西宁 810016;2.青海省林业科学研究所 青海高原林木遗传育种实验室,青海 西宁 810016)
Author(s):
 LIU Bao-yao12LIU Xiao-li2*WEI Hai-bin2GU Wen-yi2
 (1.College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai 810016,China; 2.Qinghai Forestry Research Institute/Qinghai Plateau Key Laboratory of Tree Genetics and Breeding,Xining,Qinghai 810016,China)
关键词:
 青海核桃ISSR分子标记遗传多样性
Keywords:
 Qinghai walnut ISSR molecular marker genetic diversity
分类号:
S722
DOI:
10.3969/j.issn.1001-7461.2017.02.22
文献标志码:
A
摘要:
 以青海省不同核桃分布区的222株地方核桃嫩叶为试验材料,对其进行ISSR分子标记遗传多样性研究。结果表明,12条引物共获得167个条带,其中多态性位点127个。供试材料物种水平上的多样性指数为:多态性位点127个,多态性位点百分率达76.05%,等位基因数1.760 5,有效等位基因数1.384 9,遗传多样度指数(H)0.233 2,香农指数(I)0.357 7。基于Nei’s(1978)遗传多样性分析得出的居群间遗传分化系数(Gst)为0.081 7,表明有8.17%的遗传分化存在于居群之间,说明核桃居群之内的遗传分化高于居群之间。遗传距离平均值为0.025 7,估测的居群间基因流为5.619 8,表明供试核桃居群间存在较大的基因交流。AMOVA方差分析也表明核桃的遗传变异主要分布于居群内。
Abstract:
 The paper aimed to analyze the genetic diversity of walnut in Qinghai by ISSR molecular marker in 222 walnut leaf samples collected from deferent eco-geographical regions in Qinghai.The results showed that 167 bands were amplified by 12 primers,of which 127 were polymorphic loci.At species level,the number of polymorphic loic was 127,the percentage of polymorphic loci was 76.05%,observed number of alleles was 1.760 5,effective alleles was 1.384 9,Neis gene diversity was 0.233 2,Shannons Information index(I) was 0.357 7.Genetic coefficient of differentiation among populations based Neis gene diversity was 0.081 7.It showed that 8.26% genetic differentiation existed among populations,the genetic differentiation within populations was higher than that among populations.The mean value of genetic distance was 0.025 7,estimation of gene flow from Gst was 5.619 8.It indicated that there was a large gene exchange among the populations of walnut in Qinghai.Analysis of AMOVA also demonstrated that genetic variance among populations was larger than within a population.

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备注/Memo

备注/Memo:
 收稿日期:2016-08-08修回日期:2016-10-29
基金项目:青海省科技成果转化专项“优质早实薄皮核桃丰产栽培技术集成与推广”(2016-NK-133)。
作者简介:刘宝尧,男,在读硕士,研究方向:植物资源保护与利用。E-mail:liubayao681@126.com
*通信作者:刘小利,女,研究员,研究方向:经济林。E-mail:xiaoli2408@sohu.com
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