[1]薛加楠,崔小月,张琪,等. 3种不同地理起源葡萄种质资源的抗旱性比较[J].西北林学院学报,2017,32(2):136-142.[doi:10.3969/j.issn.1001-7461.2017.02.23]
　XUE Jia-nan,CUI Xiao-yue,ZHANG Qi,et al. Comparison of Drought Tolerance of Three Grape Germplasm Resources from Different Geographic Origins[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(2):136-142.[doi:10.3969/j.issn.1001-7461.2017.02.23]
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 3种不同地理起源葡萄种质资源的抗旱性比较()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第2期
页码:
136-142
栏目:
出版日期:
2017-03-31

文章信息/Info

Title:
 Comparison of Drought Tolerance of Three Grape Germplasm Resources from Different Geographic Origins
文章编号:
1001-7461(2017)02-0136-07
作者:
 薛加楠; 崔小月; 张琪; 吴梦晓; 张剑侠*
 （西北农林科技大学 园艺学院；旱区作物逆境生物学国家重点实验室；农业部 西北园艺植物种质资源利用重点开放实验室，陕西 杨陵 712100）
Author(s):
 XUE Jia-nan; CUI Xiao-yue; ZHANG Qi; WU Meng-xiao; ZHANG Jian-xia*
 (College of Horticulture,State Key Laboratory of Crop Stress Biology in Arid Areas,Key Laboratory of Northwest Horticulture Plant Germplasm and Application of Ministry of Agriculture,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 ; 葡萄; 种质资源; 试管苗; 聚乙二醇（PEG-6000）; 干旱胁迫; 抗旱性
Keywords:
 ; Vitis;  germplasm resource;  tube seedling;  PEG-6000;  drought stress;  drought tolerance
分类号:
S722
DOI:
10.3969/j.issn.1001-7461.2017.02.23
文献标志码:
A
摘要:
 研究3种不同地理起源的葡萄种质资源河岸葡萄‘河岸（♀）’、燕山葡萄‘燕山-1’和欧洲葡萄‘红地球’的抗旱性差异，为进一步挖掘葡萄的抗旱基因提供依据。以3种不同葡萄的试管苗为材料，在培养基中加入PEG-6000模拟干旱胁迫，研究其形态变化和6项生理生化指标的变化，进而评价其抗旱性。结果表明，干旱胁迫后，3种葡萄试管苗叶片边缘开始出现干枯的时间不同，‘河岸（♀）’、‘红地球’和‘燕山-1’分别出现于3、12 h和27 h；随着干旱胁迫时间的延长，受害程度最大的是‘河岸（♀）’，其次是‘红地球’，‘燕山-1’仅有轻微的伤害。叶片中脯氨酸含量总体呈上升趋势，胁迫72 h时，‘燕山-1’分别是‘红地球’和‘河岸（♀）’的1.14倍和4.84倍；POD、CAT和SOD含量呈先升高后下降趋势，72 h时，‘燕山-1’的POD含量分别是‘红地球’和‘河岸（♀）’的6.2倍和49.2倍，CAT含量分别是‘红地球’和‘河岸（♀）’的0.44倍和1.47倍，SOD含量分别是‘红地球’和‘河岸（♀）’的1.95倍和1.05倍；超氧阴离子自由基含量（O·-2）呈先升高后下降趋势，72 h时，‘燕山-1’的O·-2含量分别是‘红地球’和‘河岸（♀）’的1.9倍和3.0倍； MDA含量呈先升高后下降趋势，72 h时‘燕山-1’分别是‘红地球’和‘河岸（♀）’的1.4倍和1.26倍。综合形态变化及各项生理生化指标的变化，以‘燕山-1’的抗旱性最强，其次是‘红地球’，抗旱性最差的是‘河岸（♀）’。
Abstract:
 The objective of this study was to examine the differences in drought resistance of three grapevines from different geographic origins,including Vitis yeshanensis accession ‘Yanshan-1’ (Y),Vitis riparia accession ‘Hean (♀)’ (H) and Vitis vinifera cv.‘Red Globe’ (R).The plantlets of the three were used as materials,the changes of morphology,proline,MDA,the activities of protective enzymes and superoxide anion free radicals contents in the blades were investigated under the stress of PEG-6000.The occurrence of the dry spots in the blades was different among the three: the spots appeared 3,12 and 27 hours after the stress for H,R,and Y,respectively.With the increase of drought stress time,H was injured mostly,followed by R,and Y was slightly injured.The proline contents in the blades of the three increased with time.Seventy-two hours after the stress,the proline content in the blades of Y was 1.14 and 4.48 folds to R and H,respectively.The activities of POD,CAT and SOD initially presented an upward trend but all declined later.Seventy-two hours after the stress,the POD activity of Y was 6.2 and 49.2 folds to R and H,the CAT activity of Y was 0.44 and 1.47 folds R and H,and the SOD activity of Y was 1.95 and 1.05 folds to R and H,respectively.The superoxide anion free radicals content initially increased,and then decreased.At 72 h after the stress,the superoxide anion free radicals content of Y was 1.9 and 3.0 folds to R and H,respectively.The MDA content initially increased,but declined later.At 72 h after stress,the MDA content of Y was 1.4 and 1.26 folds to R and H,respectively.Considering morphological changes and the changes of the physiological and biochemical indexes comprehensively,Y demonstrated the highest drought resistance,followed by R and H.

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备注/Memo

备注/Memo:
 收稿日期：2016-06-06修回日期：2016-10-31
基金项目：国家科技支撑计划子课题（2013BAD02B04-06）。
作者简介：薛加楠，女，在读硕士，研究方向：葡萄种质资源与生物技术。E-mail：xuejn2016@sina.com
*通信作者：张剑侠，男，教授，博士生导师，研究方向：葡萄种质资源与生物技术。E-mail:zhangjx666@126.com
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