[1]刘晓娇,李彬,王娟,等. 商洛产粗榧叶总黄酮提取工艺和抗氧化活性研究[J].西北林学院学报,2017,32(2):225-229.[doi:10.3969/j.issn.1001-7461.2017.02.38]
　LIU Xiao-jiao,LI Bin,WANG Juan,et al. Extraction Process and Antioxidant Activity of Total Flavononoids of Cephalotaxus sinensis Leaves from Shangluo[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(2):225-229.[doi:10.3969/j.issn.1001-7461.2017.02.38]
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 商洛产粗榧叶总黄酮提取工艺和抗氧化活性研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第2期
页码:
225-229
栏目:
出版日期:
2017-03-31

文章信息/Info

Title:
 Extraction Process and Antioxidant Activity of Total Flavononoids of Cephalotaxus sinensis Leaves from Shangluo
文章编号:
1001-7461(2017)02-0225-05
作者:
 刘晓娇1; 2; 李彬1; 王娟3; 吕欣2*
 （1.商洛学院 生物医药与食品工程学院，陕西 商洛 726000；2.西北农林科技大学 食品科学与工程学院，陕西 杨陵 712100；3.西北农林科技大学 动物医学院，陕西 杨陵 712100）
Author(s):
 LIU Xiao-jiao1; 2; LI Bin1; WANG Juan3; LYU Xin2*
 (1.School of Biomedicine and Food Engineering,Shangluo University,Shangluo,Shaanxi 726000,China; 2.College of Food Science and Engineering,Northwest A&F University,Yangling,Shaanxi 712100,China; 3.College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 ; 粗榧; 黄酮; 乙醇浸提; 正交试验
Keywords:
 ; Cephalotaxus sinensis;  flavonoid;  ethanol extraction process;  orthogonal experiment
分类号:
S718.43
DOI:
10.3969/j.issn.1001-7461.2017.02.38
文献标志码:
A
摘要:
 对商洛产粗榧叶总黄酮乙醇浸提工艺的研究，并测定其抗氧化活性。以总黄酮得率为依据，通过提取时间、料液比、乙醇浓度、提取温度的单因素和正交试验确定最佳醇提工艺，以1,1-二苯基-2-三硝基苯肼（DPPH,C18H12N5O6）清除率测定总黄酮的抗氧化活性。结果表明，粗榧叶中乙醇浸提工艺的最佳条件为： 55%乙醇（V/V）80℃提取2.5 h，料液比1∶15、浸提3次。粗榧叶中总黄酮得率5.910%，且具有良好的抗氧化活性。
Abstract:
 The aims of this study were to establish optimal ethanol extraction process from the leaves of Cephalotaxus sinensis,and to measure the antioxidant activity of the extracts.The optimal ethanol extraction conditions were obtained as ethanol concentration: 55% (V/V),extraction temperature: 85℃,extraction time: 2.5 h,material to solvent ratio: 1∶15,times of extraction: 3,by which the extraction yield of total flavonoids was 5.910%,and presenting good anti-oxidant activity.

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备注/Memo

备注/Memo:
 收稿日期：2016-07-15修回日期：2016-07-26
基金项目：商洛学院2012年服务地方经济社会发展专项项目（12sky-fwdf007）。
作者简介：刘晓娇，女，讲师，在读博士，研究方向：食品分析与检测。E-mail:78xlj@163.com
*通信作者：吕欣，男，博士生导师，研究方向：食品微生物。E-mail:xinlu@nwsuaf.edu.cn
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