[1]闫文涛,佟兆庆,魏俊,等. 不同生长调节剂对蒙古栎嫩枝扦插生根的影响[J].西北林学院学报,2017,32(3):116-121.[doi:10.3969/j.issn.1001-7461.2017.03.22]
　YAN Wen-tao,TONG Zhao-qing,WEI Jun,et al. Cuttage Propagation Technique and Rooting Mechanism of Quercus mongolica with Softwood Cutting[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(3):116-121.[doi:10.3969/j.issn.1001-7461.2017.03.22]
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 不同生长调节剂对蒙古栎嫩枝扦插生根的影响()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第3期
页码:
116-121
栏目:
出版日期:
2017-05-31

文章信息/Info

Title:
 Cuttage Propagation Technique and Rooting Mechanism of Quercus mongolica with Softwood Cutting
文章编号:
1001-7461(2017)03-0116-06
作者:
 闫文涛; 佟兆庆; 魏俊; 康德星; 陆秀君*
 （沈阳农业大学 林学院，辽宁 沈阳 110866)
Author(s):
 YAN Wen-tao; TONG Zhao-qing; WEI Jun; KANG De-xing; LU Xiu-jun*
 （College of Forestry,Shenyang Agricultural University,Shenyang,Liaoning 110161,China）
关键词:
 ; 蒙古栎; 扦插; 繁殖; 生长调节剂
Keywords:
Quercus mongolica;  cuttage propagation;  growth regulator
分类号:
S723.132.1
DOI:
10.3969/j.issn.1001-7461.2017.03.22
文献标志码:
A
摘要:
 为建立蒙古栎扦插繁殖技术体系，探究其嫩枝扦插的生根机理。以当年生蒙古栎中上部嫩枝条做插穗，采用ABT 1号、IBA、NAA及IBA+NAA混合溶液处理，分别采取200、300、500 mg·L-1与1 000 mg·L-1浓度处理不同的时间，以清水处理为对照。采用高效液相色谱法对其生根过程中吲哚乙酸（IAA）、脱落酸（ABA）、玉米素（ZT）、赤霉酸（GA3）4种内源激素含量进行测定。结果表明，用生长调节剂IBA 1 000 mg·L-1处理10 s和IBA+NAA 1 000 mg·L-1处理10 s可有效促进愈伤组织发育，但生根率仅为3.3%。在生根过程中IAA与ZT均可有效促进愈伤组织而促进生根，ABA抑制生根，低浓度GA3有利于不定根的形成。插穗的愈伤组织发生能力、生根能力与IAA/ABA比值和ZT/ABA比值呈正相关。
Abstract:
 In order to establish an efficient cutting propagation system,the rooting mechanism of softwood cuttings of Quercus mongolica was explored.The current year shoots in the middle and upper branches of Q.mongolica were used as cuttings,which were treated by different growth regulators of ABT No.1,IBA,NAA,and mixed IBA+NAA with gradient concentrations of 200,300,500 mg·L-1,and 1 000 mg·L-1 for different time durations,and water was used as the control.Four endogenous hormones,i.e.,indoleacetic acid (IAA),abscisic acid (ABA),zeatin (ZT),and gibberellin (GA3) during rooting were determined by high performance liquid chromatography (HPLC).The results showed that the treatment with IBA 1 000 mg·L-1 for 10 s and IBA+NAA 1 000 mg·L-1 for 10 s could promote the callus development,but the rooting rate was only 3.3%.In the process of rooting,IAA and ZT could promote the development of callus and promote the rooting.ABA inhibited the rooting,while low level of GA3 was beneficial to adventitious root formation.There was a positive correlation between IAA/ABA ratio and ZT/ABA ratio in callus growth and rooting ability.

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备注/Memo

备注/Memo:
 收稿日期：2016-10-17修回日期：2016-12-08
基金项目：辽宁省农业科技攻关项目（2014207005/2015207005）。
作者简介：闫文涛，男，在读硕士，研究方向：园林植物栽培与应用。E-mail：961814081@qq.com
*通信作者：陆秀君，女，教授，博士生导师，研究方向：观赏树木引种、栽培生理生态。E-mail:lxjsyau@126.com
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