[1]王丹丹,杨东霞. 软枣猕猴桃EST-SSR引物开发及通用性研究[J].西北林学院学报,2017,32(4):147-152.[doi:10.3969/j.issn.1001-7461.2017.04.26]
 WANG Dan-dan,YANG Dong-xia. Development and Transferability of EST-SSR Primers in Actinidia arguta[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(4):147-152.[doi:10.3969/j.issn.1001-7461.2017.04.26]
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 软枣猕猴桃EST-SSR引物开发及通用性研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第4期
页码:
147-152
栏目:
出版日期:
2017-07-31

文章信息/Info

Title:
 Development and Transferability of EST-SSR Primers in Actinidia arguta
文章编号:
1001-7461(2017)04-0147-06
作者:
 王丹丹杨东霞
 (辽东学院 农学院,辽宁 丹东 118003)
Author(s):
 WANG Dan-danYANG Dong-xia
 (College of Agriculture,Eastern Liaoning University,Dandong,Liaoning 118003,China)
关键词:
 软枣猕猴桃EST-SSR多态性通用性聚类分析
Keywords:
Actinidia arguta EST-SSR polymorphism transferability cluster analysis
分类号:
S663.4
DOI:
10.3969/j.issn.1001-7461.2017.04.26
文献标志码:
A
摘要:
 本研究开发软枣猕猴桃EST-SSR引物196对,探究软枣猕猴桃EST-SSR引物在猕猴桃属25个品种中的通用性,并对上述25个品种进行聚类分析。结果表明,筛选得到35对EST-SSR引物,共扩增189条带,平均每对引物扩增5.40条带,条带大小为170~250 bp,其中多态性条带达178条(94%),可见软枣猕猴桃EST-SSR引物在猕猴桃属中具有较高的通用性,猕猴桃属植物具有较高的遗传多样性;聚类分析表明,25个猕猴桃属品种的遗传相似系数(GS)为0.591~0.969,在相似系数0.70处,中华猕猴桃与美味猕猴桃归为一支,二者亲缘关系较近。
Abstract:
 EST-SSR primers with the number of 196 pairs of Actinidia arguta were developed to screen the transferability of the primers to 25 Actinidia accessions,also cluster analysis of the 25 accessions was constructed.Thirty five pairs of EST-SSR primers were screened out.A total of 189 bands were amplified,with a mean of 5.40bands per primer.The products were 170-250 bp,in which the polymorphic bands were 178 (94%),indicating the genetic diversity of Actinidia was high,and the A.arguta EST-SSR primers was highly transferable too.Cluster analysis indicated that the genetic similarity coefficient (GS) of 25 Actinidia accessions ranged from 0.591 to 0.969.At the similarity coefficient of 0.70,A.chinensis and A.deliciosa were classified as one branch,showed the close relationship between the two species.

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备注/Memo

备注/Memo:
 收稿日期:2016-11-25修回日期:2016-12-09
基金项目:国家自然科学基金(31370400);丹东市2016科技攻关项目(2016KJ001),辽东学院青年基金项目(2015QN006)。
作者简介:王丹丹,女,讲师,研究方向:生物化学与分子生物学。E-mail:wangdandansq@sina.com
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