[1]齐凤慧,陈思齐,付德山,等.水曲柳FmSOC1基因烟草中遗传转化[J].西北林学院学报,2017,32(5):98-103.[doi:10.3969/j.issn.1001-7461.2017.05.17]
　QI Feng-hui,CHEN Si-qi,FU De-shan,et al. Genetic Transformation of SOC1 Gene from Ash to Tobacco[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(5):98-103.[doi:10.3969/j.issn.1001-7461.2017.05.17]
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水曲柳FmSOC1基因烟草中遗传转化()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第5期
页码:
98-103
栏目:
出版日期:
2017-09-30

文章信息/Info

Title:
 Genetic Transformation of SOC1 Gene from Ash to Tobacco
文章编号:
1001-7461(2017)05-0098-06
作者:
 齐凤慧1; 2; 陈思齐1; 付德山1; 孟祥宇1; 詹亚光1; 2*
 （1.东北林业大学 生命科学学院，黑龙江 哈尔滨 150040；2.东北林业大学 林木遗传育种国家重点实验室，黑龙江 哈尔滨 150040）
Author(s):
 QI Feng-hui1; 2; CHEN Si-qi1; FU De-shan1; MENG Xiang-yu1; ZHAN Ya-guang1; 2*
 (1.College of Life Sciences,Northeast Forestry University,Harbin,Heilongjiang 150040,China; 2.State Key of Laboratory of Tree Breeding (NEFU),Harbin,Heilongjiang 150040,China)
关键词:
 ; 水曲柳; SOC1; 烟草; 遗传转化
Keywords:
Fraxinus mandshurica;  SOC1 gene;  tobacco;  genetic transformation
分类号:
S792.12
DOI:
10.3969/j.issn.1001-7461.2017.05.17
文献标志码:
A
摘要:
 为研究水曲柳SOC1基因对植物开花的调控功能，将水曲柳SOC1基因通过农杆菌介导法转入模式植物烟草中。利用PCR及Southern杂交法检测，结果有10株被确定为转基因植株。利用RT-PCR法对5株转基因烟草进一步分析表明，SOC1基因在3株烟草中表达。表型分析表明，转基因烟草植株生长较快，叶片窄小，开花时间提前。
Abstract:
 To study the function of gene SOC1 on regulating flowering time of plant,a SOC1 gene cloned from Fraxinus mandshurica was transformed into tobacco by an Agrobacterium tumefaciens-mediated method.The results from PCR and Southern hybridization showed that 5 lines of transgenic tobacco were obtained.RT-PCR detection showed that the SOC1 was expressed in the 3 tobacco lines.Compared to the control,transgenic tobacco lines had some different features,including faster growth,narrower leaves,and earlier flowering time.

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备注/Memo

备注/Memo:
 收稿日期：2016-12-20修回日期：2017-04-06
基金项目：国家“十二五”支撑项目“水曲柳和白桦种子园1.5代种子园及优良繁殖材料规模化生产技术研究”（2012BAD21B0201-08）。
作者简介：齐凤慧，女，博士，高级工程师，研究方向：植物基因工程及植物细胞工程。E-mail:qifenghui2001@126.com
*通信作者：詹亚光，男，博士，教授，博士生导师，研究方向：植物基因工程、林木育种等。E-mail:yaguangzhan@126.com
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