[1]刘国花,兰建彬*,刘奕清,等. 外源钙对低温胁迫下尾巨桉幼苗生理特性及内源激素的影响[J].西北林学院学报,2017,32(6):101-106.[doi:10.3969/j.issn.1001-7461.2017.06.15]
　LIU Guo-hua,LAN Jian-bin*,LIU Yi-qing,et al. Effects of Exogenous Calcium on Seedling Physiological Characteristics and Endogenous Hormone of Eucalyptus urophylla×E.grandis under Low Temperature Stress[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(6):101-106.[doi:10.3969/j.issn.1001-7461.2017.06.15]
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 外源钙对低温胁迫下尾巨桉幼苗生理特性及内源激素的影响()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第6期
页码:
101-106
栏目:
出版日期:
2017-11-30

文章信息/Info

Title:
 Effects of Exogenous Calcium on Seedling Physiological Characteristics and Endogenous Hormone of Eucalyptus urophylla×E.grandis under Low Temperature Stress
文章编号:
1001-7461(2017)06-0101-06
作者:
 刘国花1; 兰建彬2*; 刘奕清2; 朱菁1; 郑春花1
 (1.重庆文理学院 林学与生命科学学院，重庆 402160；2.重庆文理学院 特色植物研究院，重庆 402160)
Author(s):
 LIU Guo-hua1; LAN Jian-bin2*; LIU Yi-qing2; ZHU Jing1; ZHENG Chun-hua1
 (1.College of Life Science & Forestry,Chongqing University of Arts and Science,Chongqing 402160,China; 2.Research Institute of the Characteristics Plant,Chongqing University of Arts and Science,Chongqing 402160,China)
关键词:
 ; 尾巨桉; 外源钙; 低温胁迫; 抗氧化酶; 内源激素
Keywords:
Eucalyptus grandis;  calcium;  low temperature stress;  antioxidant enzyme;  endogenous hormone
分类号:
S792.39
DOI:
10.3969/j.issn.1001-7461.2017.06.15
文献标志码:
A
摘要:
 以1年生尾巨桉无性系幼苗为试验材料，在4℃低温条件下，研究外源Ca2+对尾巨桉幼苗丙二醛、渗透调节物质（脯氨酸、可溶性糖）、抗氧化酶（CAT、SOD、POD）活性及内源激素（CTK、GA、ABA）含量的影响。结果表明，在低温胁迫下尾巨桉幼苗生物量、CTK、ABA和GA含量显著低于常温处理，MDA、脯氨酸和可溶性糖含量显著高于常温处理。与不加钙处理相比，低温胁迫下，加CaCl2处理显著降低了尾巨桉幼苗MDA含量及ABA水平，而脯氨酸、可溶性糖、CTK、GA含量及CAT、POD和SOD保护酶活性则显著升高，其中10 mmol·L-1CaCl2处理尾巨桉的效果显著优于其他浓度Ca2+的处理。低温胁迫下尾巨桉幼苗的生长受到抑制，适当浓度（10～20 mmol·L-1）的外源钙可以减缓低温胁迫对幼苗造成的伤害，从而提高桉树幼苗对低温胁迫的适应能力。
Abstract:
 Exogenous Ca2+ with different concentrations were sprayed on the 1-year-old seedlings of Eucalyptus urophylla×E.grandis,and the effects of exogenous Ca2+ on MDA,osmotic adjustment substances (proline,soluble sugar),activities of antioxidant enzymes (CAT,SOD,POD) and endogenous hormones (CTK,GA,and ABA) were researched under the stress of 4℃.The biomass and CTK,ABA and GA contents of the seedlings decreased significantly and the contents of MDA,proline and soluble sugar increased dramatically under cold stress compared to the treatment of normal temperature.Compared with the absence of calcium,the calcium significantly reduced the contents of MDA,ABA levels and dramatically increased the contents of proline,soluble sugar,CTK,and GA and the activities of CAT,POD,SOD under low temperature stress.The addition of 10 mmol·L-1 CaCl2 showed a better effect than other concentrations of calcium.The seedlings growth under low temperature stress was restrained.The appropriate concentration (10-20 mmol·L-1) of calcium reduced the harm of low temperature on seedlings,meanwhile exogenous calcium enhanced the cold tolerance of the seedlings.

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备注/Memo

备注/Memo:
 收稿日期：2017-02-15修回日期：2017-07-03
基金项目：国家自然科学基金(31340016)；重庆文理学院校级项目（Z2012LX07）；重庆市科委项目（cstc2012jjA80018，cstc2013jcyjA80035）；重庆市教委项目（KJ131210）。
作者简介：刘国花，女，副教授，研究方向：植物生物技术。E-mail：hongdou58771@sina.com
*通信作者：兰建彬，男，博士，副教授，研究方向：植物低温逆境分子生物学。E-mail:lanjb1013@163.com
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