[1]杨璟,林连男,王瑞琦,等. 中华散尾鬼笔培养基筛选及培养条件优化[J].西北林学院学报,2017,32(6):145-149.[doi:10.3969/j.issn.1001-7461.2017.06.22]
 YANG Jing,LIN Lian-nan,WANG Rui-qi,et al. Screening and Optimization of Culture Medium and Conditions of Lysurus mokusin[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(6):145-149.[doi:10.3969/j.issn.1001-7461.2017.06.22]
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 中华散尾鬼笔培养基筛选及培养条件优化()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第6期
页码:
145-149
栏目:
出版日期:
2017-11-30

文章信息/Info

Title:
 Screening and Optimization of Culture Medium and Conditions of Lysurus mokusin
文章编号:
1001-7461(2017)06-0145-05
作者:
 杨璟林连男王瑞琦张国珍张晓波张国财*
 (东北林业大学 林学院,黑龙江 哈尔滨 150040)
Author(s):
 YANG JingLIN Lian-nanWANG Rui-qiZHANG Guo-zhenZHANG Xiao-boZHANG Guo-cai*
 (School of Forestry,Northeast Forestry University,Harbin,Heilongjinag 150040,China)
关键词:
 中华散尾鬼笔培养基培养条件生长速率
Keywords:
Lysurus mokusin culture medium culture condition screen optimization
分类号:
S789.9
DOI:
10.3969/j.issn.1001-7461.2017.06.22
文献标志码:
A
摘要:
 以野生中华散尾鬼笔为研究对象,对其菌丝进行分离纯化,以菌丝的生长速率为指标,对中华散尾鬼笔的培养基及培养条件进行优化,筛选出适宜中华散尾鬼笔菌丝生长的基础培养基,优化碳源、氮源、无机盐添加量及pH、培养温度等培养条件。结果表明,中华散尾鬼笔适宜培养基配方(每L)为:马铃薯200 g、葡萄糖20 g、蛋白胨3 g、琼脂15 g、10%无机盐溶液(KH2PO4 1 g、K2HPO4 1 g、MgSO4 0.5 g、FeSO4 0.01 g、NaNO3 0.5 g、CaCl2 0.05 g、KCl 0.5 g溶于100 mL蒸馏水);最佳培养条件pH为5.0、温度22℃,在此培养条件下,中华散尾鬼笔菌丝的净生长速率为0.59 cm·d-1,与培养基A相比增长23.69%。
Abstract:
Lysurus mokusin extract has insecticidal and antifungal activities,and it is necessary to cultivate a large number of mycelium because its mycelia grow slowly in the laboratory environment.In order to improve the growth rate of Lysurus mokusin mycelia,the culture medium and conditions were screened and optimized in this study.The culture medium was screened with the growth rate of mycelium as the evaluation index,and the suitable medium for the growth of L.mokusin mycelia was selected out.And on this basis,the carbon source,nitrogen source,inorganic salt addition,pH and culture temperature were further optimized.The best culture medium was 200 g of potato,20 g of glucose,3 g of peptone,10% inorganic salt solution (KH2PO4 1 g,K2HPO4 1 g,MgSO4 0.5 g,CaCl2 0.05 g,KCl 0.5 g,FeSO4 0.01 g and NaNO3 0.5 g dissolved in 100 mL distilled water) and 15 g of agar.The optimum culture conditions were pH=5 and temperature of 20℃.Under the above culture conditions,the net growth rate of L.mokusin mycelia was 0.59 cm/d,which was 23.69% higher than that of medium A.

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备注/Memo

备注/Memo:
 收稿日期:2017-06-19修回日期:2017-08-18
基金项目:中央高校基本科研业务费专项资金项目(2572016AA03);哈尔滨市应用技术研究与开发项目(2016RAXXJ035);黑龙江省森工总局科研项目(sgzjT2015005);中央高校基本科研业务费专项资金项目(2572014AA08)。
作者简介:杨璟,女,在读硕士,研究方向:森林有害生物综合防治、微生物源农药。E-mail:pheureonyang@nefu.edu.cn
*通信作者:张国财,男,教授,博士生导师,研究方向:森林有害生物综合管理、植物源农药、微生物源农药。E-mail:Zhang640308@126.com
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