[1]袁艮霞,李思南,秦辰,等. 苹果树腐烂病菌挥发性代谢产物的分离提取与致病性研究[J].西北林学院学报,2018,33(1):211-215.[doi:10.3969/j.issn.1001-7461.2018.01.35]
　YUAN Gen-xia,LI Si-nan,QIN Chen,et al. Isolation,Extraction and Pathogenicity Studies of Volatile Metabolites from Valsa mali[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2018,33(1):211-215.[doi:10.3969/j.issn.1001-7461.2018.01.35]
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 苹果树腐烂病菌挥发性代谢产物的分离提取与致病性研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第33卷
期数:
2018年第1期
页码:
211-215
栏目:
出版日期:
2018-01-31

文章信息/Info

Title:
 Isolation,Extraction and Pathogenicity Studies of Volatile Metabolites from Valsa mali
文章编号:
1001-7461(2018)01-0211-05
作者:
 袁艮霞1; 李思南2; 秦辰3; 王惠1*; 黄丽丽2*
 （1.西北农林科技大学 理学院，陕西 杨陵 712100；2.西北农林科技大学 植物保护学院，陕西 杨陵 712100；3.西北农林科技大学 农学院，陕西 杨陵 712100）
Author(s):
 YUAN Gen-xia1; LI Si-nan2; QIN Chen3; WANG Hui1*; HUANG Li-li2*
 (1.College of Science,Northwest A&F University,Yangling,Shaanxi 712100,China; 2.College of Plant Protection,Northwest A&F University ,Yangling,Shaanxi 712100,China;3.College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 ; 苹果树腐烂病菌; 挥发性代谢产物; 致病性; GC-MS
Keywords:
Valsa mali;  volatile metabolites;  pathogenicity;  GC-MS
分类号:
S436.611
DOI:
10.3969/j.issn.1001-7461.2018.01.35
文献标志码:
A
摘要:
 为了研究苹果树腐烂病菌的致病活性物质，本研究利用MS液体培养基培养苹果树腐烂病菌，采用普通蒸馏法用乙酸乙酯作溶剂，收集其培养滤液中的挥发性代谢产物，利用气相色谱质谱联用仪（GC-MS）分离各个成分，将各组分质谱图与数据库标准物质质谱图对比，初步进行结构定性，用苹果枝条进行致病活性测定。结果表明，苹果树腐烂病菌在发酵过程中产生的2种挥发性物质的结构分别为异戊醇和2-苯乙醇，这2种物质均对苹果树枝具有致病活性。
Abstract:
Valsa mali was cultured in MS liquid medium to explore its pathogenic active substances.Its volatile metabolites were collected by distillation method with ethyl acetate as absorbing solvent.Volatile constituents dissolved in ethyl acetate were separated and identified by GC-MS.To confirm the structure of each component,their mass spectra were compared with those of standard substances in the database,and their bioactivities were tested on apple branches.Two substances obtained from the culture filtrate were 3-methylbutan-1-ol and 2-phenylethan-1-ol respectively.Both of these substances showed pathogenic activity against apple branches.

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备注/Memo

备注/Memo:
 收稿日期：2017-03-02修回日期：2017-05-13
基金项目：国家重点研发计划(2016YFD0201108)；陕西省科技统筹计划。
作者简介：袁艮霞，女，在读硕士，研究方向：天然产物。E-mail：1226206475@qq.com
*通信作者：王惠，女，博士，教授，硕士生导师， 研究方向：天然农用活性物质。E-mail：wanghuiab@sina.com黄丽丽，女，硕士，教授，博士生导师，研究方向：植物病害综合防治。E-mail：lilyhuangk@163.com
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