[1]王晓丽,李文静,张志强,等. 渗透胁迫对云南松幼苗生物量及碳酸酐酶的影响[J].西北林学院学报,2018,33(2):10-15.[doi:10.3969/j.issn.1001-7461.2018.02.02]
 WANG Xiao-li,LI Wen-jing,ZHANG Zhi-qiang,et al. Affects of Osmotic Stress on the Biomass and Carbonic Anhydrase of Pinus yunnanensis Seedlings[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2018,33(2):10-15.[doi:10.3969/j.issn.1001-7461.2018.02.02]
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 渗透胁迫对云南松幼苗生物量及碳酸酐酶的影响()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第33卷
期数:
2018年第2期
页码:
10-15
栏目:
出版日期:
2018-03-31

文章信息/Info

Title:
 Affects of Osmotic Stress on the Biomass and Carbonic Anhydrase of Pinus yunnanensis Seedlings
文章编号:
1001-7461(2018)02-0010-06
作者:
 王晓丽1李文静2张志强1曾仰君2曹子林3*
 (1.西南林业大学 林学院,云南 昆明 650224;2.昭通市林业有害生物监测检验中心,云南 昭通 657000;3.西南林业大学 环境科学与工程学院,云南 昆明 650224)
Author(s):
 WANG Xiao-li1LI Wen-jing2ZHANG Zhi-qiang1ZENG Yang-jun2CAO Zi-lin3*
 (1.College of Forestry,Southwest Forestry University,Kunming,Yunnan 650224,China; 2.Forestry Pest Monitoring Inspection Center of Zhaotong,Zhaotong,Yunnan 657000,China; 3.College of Ecology and Soil & Water Conservation,Southwest Forestry University,Kunming,Yunnan 650224,China)
关键词:
 云南松生物量碳酸酐酶渗透胁迫
Keywords:
Pinus yunnanensis biomass carbonic anhydrase osmotic stress
分类号:
S791.257.01
DOI:
10.3969/j.issn.1001-7461.2018.02.02
文献标志码:
A
摘要:
 以云南松幼苗为研究材料,通过PEG处理模拟干旱胁迫的方式,采用两因素四水平的正交试验设计,探讨不同浓度PEG6000处理不同天数对云南松幼苗生物量及碳酸酐酶活性的影响。结果表明,1)随着渗透胁迫程度的加深,云南松幼苗根、茎、叶及总生物量鲜重总体上皆呈现下降的趋势,但处理13的茎、叶及总生物量皆高于其他的渗透胁迫处理,而该处理的根生物量是下降的,表明大强度短时间的渗透胁迫对根生长的抑制效应大于其对茎和叶的抑制。2)处理9渗透胁迫下,云南松幼苗生物量分配中根所占比例最高,为23.29%,说明一定强度的渗透胁迫有利于云南松幼苗对根生物量比的调整,从而有利于从生物量分配调整方面增强其抗逆能力。3)云南松幼苗碳酸酐酶(CA)相对活性随着渗透胁迫程度的增大呈先增强后减弱的趋势;所有胁迫处理的CA活性皆大于对照,且处理11中CA活性最大;不同处理间的CA活性存在极显著差异;渗透胁迫诱导了CA活性的增强,有利于提高云南松幼苗的抗逆能力。4)影响碳酸酐酶相对活性的主要因子是因素A(PEG6000的浓度),因素A水平间对酶活性具有极显著的影响,酶活性随着处理液浓度的升高呈先升高后下降的情况,X3(20%的PEG6000)处理浓度下酶活性最高,因此该渗透胁迫强度应是云南松调节碳酸酐酶相对活性提高其抗逆能力的转折点。
Abstract:
 Taking the seedlings of Pinus yunnanensis as materials,the biomass and carbonic anhydrase activity of the seedlings in different osmotic stress intensities that were simulated by PEG6000 were analyzed by a 2-factor and 4-level orthogonal design experiment.The results showed that 1) the fresh weights of roots,stems,leaves and total biomass decreased with the deepening of osmotic stress on the whole,while in the treatment No.13,the biomass of the seedling stems,leaves and total biomass were higher than those of other treatments,and the biomass of the roots demonstrated a decreased trend,indicating that the stress with high intensity and short duration could inhibit the root growth more significantly than those on the stems and leaves.2) The root biomass ratio in the seedlings of the treatment No.9 was the highest (23.29%),suggesting that a certain intensity of osmotic stress was beneficial to regulate the root biomass ratio in the seedlings,thus enhancing its resilience in terms of biomass allocation and adjustment.3) In a certain degree,the activities of carbonic anhydride in P.yunnanensis seedlings increased with the stress intensity and duration.However,with the further elevated stress intensity,the activities of carbonic anhydride declined.The increase of activity of carbonic anhydride could be induced by osmotic stress,which could improve the ability of tolerating adversity of P.yunnanensis seedlings.4) The main factor affecting activity of carbonic anhydride was PEG6000 concentration that had a very significant effect on enzyme activity.The activity was the highest in the treatment of X3 (20% PEG6000),which therefore was the turning point of the seedlings to regulate the activity of carbonic anhydrase for increasing the resilience of P.yunnanensis seedlings.

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备注/Memo

备注/Memo:
 收稿日期:2017-05-29修回日期:2017-08-08
基金项目:云南省高校优势特色重点学科(生态学)建设项目资助。
作者简介:王晓丽,女,副教授,研究方向:森林培育和林木生理生态。E-mail:1144607944@qq.com
*通信作者:曹子林,男,副教授,研究方向:林木生理生态。E-mail:fjcaozilin@qq.com
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