[1]李德燕,周运超*. 钙对马尾松针叶生理生化特性及细胞超微结构的影响[J].西北林学院学报,2018,33(2):20-27.[doi:10.3969/j.issn.1001-7461.2018.02.04]
　LI De-yan,ZHOU Yuan-chao*. Effects of Calcium Concentration on Physio-biochemical Characteristics and Ultrastructure of Pinus massoniana Needles[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2018,33(2):20-27.[doi:10.3969/j.issn.1001-7461.2018.02.04]
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 钙对马尾松针叶生理生化特性及细胞超微结构的影响()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第33卷
期数:
2018年第2期
页码:
20-27
栏目:
出版日期:
2018-03-31

文章信息/Info

Title:
 Effects of Calcium Concentration on Physio-biochemical Characteristics and Ultrastructure of Pinus massoniana Needles
文章编号:
1001-7461(2018)02-0020-08
作者:
李德燕1; 2; 3; 周运超1; 3*
 （1．贵州大学 贵州省森林资源与环境研究中心，贵州 贵阳 550025；2．安顺学院，贵州 安顺 561000；3．贵州大学 林学院，贵州 贵阳 550025）
Author(s):
 LI De-yan1; 2; 3; ZHOU Yuan-chao1; 3*
 （1.Institute for Forest Resources ＆ Environment of Guizhou，Guizhou University，Guiyang,Guizhou 550025,China; 2.College of Agronomy,Anshun University,Anshun,Guizhou 561000,China; 3.College of Forestry,Guizhou University,Guiyang,Guizhou 550025,China）
关键词:
 ; 马尾松; 钙浓度; 生理生化特性; 超微结构
Keywords:
Pinus massoniana;  calcium concentration;  physio-biochemical index;  ultrastructure
分类号:
S791.248
DOI:
10.3969/j.issn.1001-7461.2018.02.04
文献标志码:
A
摘要:
 以1年生马尾松（Pinus massoniana）苗为试验材料，通过温室砂培试验，研究不同钙浓度（0、0.4、1、2、3 mmol·L-1Ca2+和4 mmol·L-1Ca2+）对马尾松针叶生理生化特性和细胞超微结构的影响，阐明马尾松对钙的适应性机理，为马尾松人工林经营及管理提供理论参考。结果表明，随供钙浓度增加，马尾松苗生物量、针叶叶绿素、GA、ZR和IAA含量及叶绿素a/b值随钙浓度增加先增加后降低，在2 mmol·L-1Ca2+条件下达到最高值；针叶相对电导率、ABA含量、ABA/GA、ABA/ZR、ABA/IAA和ABA/（GA+ZR+IAA）值随钙浓度增加先降低后增加，在2 mmol·L-1Ca2+条件下达到最低值；2 mmol·L-1Ca2+处理的针叶细胞中叶绿体和线粒体形态完整，淀粉粒含量较多，嗜锇颗粒和线粒体数量相对较少，未出现质壁分离，而在其余5个处理中，针叶细胞均出现不同程度质壁分离现象，细胞器结构和功能受损，其中0 mmol·L-1Ca2+处理的细胞受损程度较重，并在部分叶绿体内出现空泡现象，而4 mmol·L-1Ca2+的细胞受损程度次之。在不同供钙水平下，马尾松苗通过调节体内激素含量及比值，稳定细胞内正常生理生化代谢进程，改善针叶的光合状况，从而保护细胞膜、细胞器的结构和功能完整，以利于自身生长发育，且Ca2+在2 mmol·L-1时对马尾松生长最有利。
Abstract:
 To provide reference for Pinus massoniana plantation management,a greenhouse sand culture experiment was designed to observe physico-biochemical responses and cell ultrastructure of P.massoniana needles in different Ca2+ concentrations (0,0.4,1,2,3 and 4 mmol·L-1) and to illuminate the adapting mechanisms of P.massoniana seedlings to different Ca2+ concentrations.The results indicated that the seedling’s biomass, needle’s chlorophyll,GA,ZR and IAA contents and ratio of chlorophyll a/b increased and then decreased with the increase of Ca2+ concentrations,and all their maximum values appeared in 2 mmol·L-1 Ca2+,but the rule was varied inversely with the needle’s relative conductivity rate,ABA content and ratio of ABA/GA,ABA/ZR,ABA/IAA and ABA/(GA+ZR+IAA).For the mesophyll cell of P.massoniana with 2 mmol·L-1Ca2+,chloroplasts and mitochondria were all complete,its content of starch was higher,osmiophilic granule and mitochondrion all had lower contents and all cells had no caused plasmolysis.Nevertheless,mesophyll cells of other 5 treatments all appeared plasmolysis at different calcium levels,and which finally caused cellular structure damage and function disorder.Mesophyll cells of P.massoniana under 0 mmol·L-1Ca2+ was most severely damaged,and appeared cavitations within chloroplasts.The next was P.massoniana with 4 mmol·L-1Ca2+ according to the degree of cellular structure damage.Thus,P.massoniana seedling treated with different Ca2+ levels could regulate endohormone contents and their comparative ratios,physiological metabolism and photosynthesis,to protect the normal structure and function of cell membrane and organelles,effectively to promote seedling growth.The treatment of 2 mmol·L-1Ca2+ concentration was the best for P.massoniana seedlings.

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备注/Memo

备注/Memo:
 收稿日期：2017-04-25修回日期：2017-09-19
基金项目：贵州省重大专项(黔科合重大专项字［2012］6001号)；“百”层次人才计划（黔科合人才（2015）4022）。
作者简介：李德燕，女，研究方向：植物生理生态。E-mail：lideyan1234@163.com
*通信作者：周运超，男，教授，博士生导师，研究方向：植物生理生态。E-mail:yczhou@gzu.edu.cn
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