[1]闫芬芬,郑兴娟,罗智,等. 枣和酸枣杂交后代遗传多样性的SSR分析[J].西北林学院学报,2018,33(3):91-97.[doi:10.3969/j.issn.1001-7461.2018.03.14]
　YAN Fen-fen,ZHENG Xing-juan,LUO Zhi,et al. Genetic Diversity Analysis of Hybrid Progeny from Chinese Jujube and Wild Jujube by SSR[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2018,33(3):91-97.[doi:10.3969/j.issn.1001-7461.2018.03.14]
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 枣和酸枣杂交后代遗传多样性的SSR分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第33卷
期数:
2018年第3期
页码:
91-97
栏目:
出版日期:
2018-05-31

文章信息/Info

Title:
 Genetic Diversity Analysis of Hybrid Progeny from Chinese Jujube and Wild Jujube by SSR
文章编号:
1001-7461(2018)03-0091-07
作者:
 闫芬芬1; 3; 郑兴娟2; 罗智2; 王玖瑞2; 3*; 刘孟军1; 3*
（1.河北农业大学 中国枣研究中心，河北 保定 071001；2.河北农业大学 林学院，河北 保定 071001; 3.河北省枣产业技术研究院,河北 保定 071001）
Author(s):
 YAN Fen-fen1; 3; ZHENG Xing-juan2; LUO Zhi2; WANG Jiu-rui2; 3*; LIU Meng-jun1; 3*
 (1.Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding,Hebei 071001,China; 2.College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071001,China; 3.Jujube Industry Technology Research Institute of Hebei,Baoding,Hebei 071001,China)
关键词:
 ; 枣; 酸枣; 杂交后代; 遗传多样性; 雄性不育; SSR
Keywords:
 ; Chinese jujube;  wild jujube;  hybrid progeny;  genetic diversity;  male sterile;  SSR
分类号:
S665.1
DOI:
10.3969/j.issn.1001-7461.2018.03.14
文献标志码:
A
摘要:
 以枣雄性不育种质‘JMS2’为母本、酸枣优系‘邢16’为父本通过人工控制杂交获得的179株杂交后代及其父母本为试验材料，利用SSR标记进行杂种鉴定及遗传多样性分析。结果表明，利用双亲差异互补SSR引物JSSR311一次性鉴定出所有子代均为杂种，实现了该组合大量杂交后代的快速、高效鉴定。随机选取21对SSR引物，在杂交后代中共扩增出46个等位基因位点，其中多态性条带24个，多态性比率52.17%。经遗传分析，杂交后代群体Shannons信息指数(I)平均值为0.64，期望杂合度(Hei)平均值为0.42，说明杂交后代群体的遗传多样性高。UPGMA聚类分析显示，杂交后代个体及亲本间相似系数介于0.64~0.97，在相似系数0.70附近可将杂交后代分为3大类，其中第1大类由172个子代与母本聚在一起、占子代总数的96%，第2大类由2个子代与父本单独聚为一类，表明杂交后代与母本JMS2的遗传相似度较高。
Abstract:
 The male sterile Chinese jujube (Ziziphus jujuba Mill.‘JMS2’) and wild jujube (Z.acidojujuba Cheng et Liu ‘Xing 16’),as well as their 179 hybrid progenies were used.SSR markers were adopted to identify the hybrids and to conduct the genetic diversity analysis.The results showed that the JSSR311,a two-parent differential primer,could quickly identify the hybrid purity of mass progeny and confirmed that all the tested progenies were hybrids.A total of 46 bands/gene loci were amplified from 21 randomly selected SSR primers，of which 24 bands were polymorphic (52.7%).The Shannons information index (I) was 0.64 and the expected heterozygosity (Hei) was 0.42,which showed a high genetic diversity in hybrid progeny.The UPGMA dendrogram demonstrated that the genetic similarity coefficient between individuals and parents ranged from 0.64 to 0.97,the hybrids could be divided into three classes at the similarity coefficient of 0.70.The first category included 172 progenies and female parent ‘JMS2’,accounting for 96% of the total number of offspring.The second category included 2 progenies and male parent wild jujube,indicating that the hybrid progeny had higher genetic similarity with the female JMS2.

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备注/Memo

备注/Memo:
 收稿日期：2017-08-17修回日期：2017-09-26
基金项目：国家科技支撑项目（2013BAD14B03）；河北省枣产业技术研究院建设引导经费（16967645D）；北京市2011协同创新中心项目（PXM2017_014207_000043）。
作者简介：闫芬芬，女，在读博士，研究方向：干果种质资源与分子辅助育种。E-mail:yanfening@163.com
*通信作者：刘孟军，男，教授，博士生导师，研究方向：枣属植物。E-mail：lmj1234567@aliyun.com；
王玖瑞，男，教授，硕士生导师，研究方向：枣种质资源与育种。E-mail：wjrjujube@126.com
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