[1]张靖雯,姜在民,蔡靖*. 紫丁香与羽叶丁香叶绿体DNA提取方法研究[J].西北林学院学报,2018,33(4):95-99.[doi:10.3969/j.issn.1001-7461.2018.04.15]
 ZHANG Jing-wen,JIANG Zai-min,CAI Jing*. Extraction Method of Chloroplast DNA of Syringa oblata and Syringa pinnatifolia[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2018,33(4):95-99.[doi:10.3969/j.issn.1001-7461.2018.04.15]
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 紫丁香与羽叶丁香叶绿体DNA提取方法研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第33卷
期数:
2018年第4期
页码:
95-99
栏目:
出版日期:
2018-07-31

文章信息/Info

Title:
 Extraction Method of Chloroplast DNA of Syringa oblata and Syringa pinnatifolia
文章编号:
1001-7461(2018)04-0095-05
作者:
 张靖雯1姜在民2蔡靖1*
 (1.西北农林科技大学 林学院,陕西 杨陵 712100;2.西北农林科技大学 生命科学学院,陕西 杨陵 712100)
Author(s):
 ZHANG Jing-wen1JIANG Zai-min2CAI Jing1*
 (1.College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China;2.College of Life Sciences ,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 紫丁香羽叶丁香叶绿体分离cpDNA提取改良高盐-低pH法
Keywords:
Syringa oblata Syringa pinnatifolia chloroplast isolation cpDNA extraction modified high-salt low-pH method
分类号:
S685.26
DOI:
10.3969/j.issn.1001-7461.2018.04.15
文献标志码:
A
摘要:
 为了探究适合丁香属植物的叶绿体DNA (cpDNA)的提取方法,采取高盐-低pH法和改良高盐-低pH法分别分离提取了紫丁香与羽叶丁香的叶绿体及cpDNA。结果表明,采取高盐-低pH法提取出的cpDNA,其OD260/OD280值均<1.7,且琼脂糖凝胶电泳检测无条带,表明cpDNA质量低,不能满足后续叶绿体基因组测序要求,改良高盐-低pH法提取出的cpDNA,其OD260/OD280值在1.8~1.9,琼脂糖凝胶电泳检测显示条带清晰,无降解现象,表明cpDNA质量高,能够满足后续叶绿体基因组测序要求。研究表明,改良高盐-低pH法可简便快速的提取紫丁香与羽叶丁香的cpDNA,为进一步研究丁香属植物的叶绿体基因组奠定了基础。
Abstract:
 To explore the suitable extraction method of chloroplast DNA (cpDNA) of Syringa L.,the chloroplast and cpDNA of Syringa oblata and S.pinnatifolia were isolated and extracted by high salt-low pH method and modified high salt-low pH method,respectively.The results showed that the value of OD260/OD280 of cpDNA extracted by the high salt-low pH method was less than 1.7,and agarose gel electrophoresis showed no cpDNA stripes,which indicated that the cpDNA was of low quality and could not meet the requirements of subsequent chloroplast genome sequencing.The value of OD260/OD280 of cpDNA extracted by the modified high salt-low pH method was between 1.8 and 1.9,and agarose gel electrophoresis showed that the cpDNA stripes were bright and no degradation,which indicated that the cpDNA was of high quality and could meet the requirements of subsequent chloroplast genome sequencing.This research showed that the cpDNA of S.oblata and S.pinnatifolia could be simply and quickly obtained by the modified high salt-low pH method,it laid the foundation for the further study of chloroplast genome of Syringa.

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备注/Memo

备注/Memo:
 收稿日期:2017-10-30修回日期:2018-01-06
基金项目:国家林业局林业公益性行业科研专项项目(201204308)。
作者简介:张靖雯,女,在读硕士,研究方向:森林植物利用与保护。E-mail:jzhangjingwen@163.com
*通信作者:蔡靖,女,教授,博士生导师,研究方向:森林植物利用与保护、植物生理生态。E-mail:cjcaijing@163.com
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