[1]肖龙敏,唐明,张好强*. 不同种植年限宁夏枸杞根际微生物的群落多样性[J].西北林学院学报,2018,33(6):31-39.[doi:10.3969/j.issn.1001-7461.2018.06.06]
　XIAO Long-min,TANG Ming,ZHANG Hao-qiang*. Diversity of Microbial Community in Rhizosphere Soils of Lycium barbarum Relative to Cultivation History[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2018,33(6):31-39.[doi:10.3969/j.issn.1001-7461.2018.06.06]
点击复制

 不同种植年限宁夏枸杞根际微生物的群落多样性()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第33卷
期数:
2018年第6期
页码:
31-39
栏目:
出版日期:
2018-11-30

文章信息/Info

Title:
 Diversity of Microbial Community in Rhizosphere Soils of Lycium barbarum Relative to Cultivation History
文章编号:
1001-7461(2018)06-0031-09
作者:
 肖龙敏; 唐明; 张好强*
 （西北农林科技大学 林学院，陕西 杨陵 712100）
Author(s):
 XIAO Long-min; TANG Ming; ZHANG Hao-qiang*
 (College of Forestry,Northwest A&F University ，Yangling,Shaanxi 712100,China)
关键词:
 ; PCR-DGGE; 宁夏枸杞; 菌根侵染率; 微生物多样性; 根际土壤
Keywords:
 ; PCR-DGGE;  Lycium barbarum;  arbuscular mycorrhizal infection rate;  microbial diversity;  rhizosphere soil
分类号:
S718.81
DOI:
10.3969/j.issn.1001-7461.2018.06.06
文献标志码:
A
摘要:
 为了解长期人工种植宁夏枸杞根际土壤微生物群落结构特征及其与AM真菌侵染率的关系，本研究分析测定了种植5、10、15 a和24 a宁夏枸杞根际AM真菌的定殖情况和土壤理化性质，并利用PCR-DGGE技术分析了不同种植年限宁夏枸杞根际土壤微生物多样性。结果表明，不同种植年限宁夏枸杞根际土壤pH、速效磷、速效钾、硝态氮存在显著差异，速效磷、速效钾、硝态氮含量在10 a达到最大，这可能与长期施肥及植物对氮磷钾的利用率有关。测序结果表明，随着种植年限的增长，宁夏枸杞根际土壤的微生物多样性呈一定程度的下降趋势，宁夏枸杞根际AM真菌的香农指数在种植5 a时最高，条带数最多，为11条，在种植10 a时显著降低（与种植5 a枸杞相比），在种植24 a时达到最低。根际细菌的香农指数在种植5 a时最高，条带数最多，为31条，说明5 a生宁夏枸杞根际根中细菌种类最多，在种植15 a时显著降低（与种植10 a相比）。通过相关性分析发现，孢子密度差异显著，孢子密度与铵态氮显著正相关，菌根侵染率与铵态氮呈负相关。AM真菌物种丰度与速效钾、硝态氮显著正相关，与土壤pH显著负相关，均匀度指数与pH正相关，与速效磷、铵态氮显著负相关。由此可见，树龄和土壤因子在一定程度上影响根际微生物群落结构。
Abstract:
 The objective of this study was to understand the microbial community characteristics of rhizosphere soil and the relation with arbuscular mycorrhizal colonization in long-term cultivation of Lycium barbarum.The AM fungus colonization status and physicochemical properties of the L.barbarum cultivation soil were analyzed.PCR-DGGE technique was applied to analyze the microbial community in the rhizosphere of L.barbarum with different cultivation time (5,10,15,and 24 years) in a nursery of Yinchuan,Ningxia,China.The results showed that soil pH,readily available phosphorus (P),readily available potassium (K),and nitrate nitrogen (N) in rhizosphere soil significantly differed amoung the soils with different cultivation time.The readily available P,readily available K,and nitrate N reached maximum at 10 years,which may due to long-term fertilization and plant utilization of N,P,and K.According to the results of sequence analysis of DGGE dominant bands,the microbial diversity of rhizosphere soil decreased as the increase of cultivation time.The Shannon-Weiner index of AM fungi from the rhizosphere soil was the highest in 5 years and had 11 bands,which significantly reduced in 10 years (compared with in 5 years) and reached the lowest in 24 years.The Shannon-Weiner index of bacteria from the rhizosphere soil was the highest in 5 years and had 31 bands,which significantly reduced in 15 years (compared with in 10 years).According to the correlation analysis,ammonium N was positively correlated with spore density,and negatively correlated with arbuscular mycorrhizal colonization.Spore density was positively correlated with ammonium N.Species richness was positively correlated withthe readily available K and nitrate N,positively correlated with pH.Evenness index was positively correlated with pH,negatively correlated with the readily available P,and ammonium N.Furthermore,plant ages and soil factors affected rhizosphere soil AM fungi community characteristics to some extent.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(6):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(6):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
[11]张帅帅,南小宁,王云果,等. 基于PCR-DGGE技术的3种植食性叶蜂幼虫肠道细菌群落结构分析[J].西北林学院学报,2017,32(5):154.[doi:10.3969/j.issn.1001-7461.2017.05.27]
　ZHANG Shuai-shuai,NAN Xiao-ning,WANG Yun-guo,et al. Gut Bacteria Flora from Three Kinds of Herbivorous Sawfly Larvae Based on PCR-DGGE Technology[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(6):154.[doi:10.3969/j.issn.1001-7461.2017.05.27]

备注/Memo

备注/Memo:
 收稿日期：2018-01-12修回日期：2018-05-09
基金项目：中国博士后基金面上项目（2016M592849）；陕西省博士后基金（2016BSHYDZZ19）；西北农林科技大学博士基本科研业务费（Z109021503）。
作者简介：肖龙敏，女，在读硕士，研究方向:森林微生物。E-mail：252979398@qq.com
*通信作者：张好强，男，副教授，研究方向：丛枝菌根真菌多样性及调控植物抗逆性。E-mail：zhanghaoqiang@nwsuaf.edu.cn
更新日期/Last Update: