[1]刘信旭,曾辉,时鹏,等. 菌酶联合法提取松墨天牛甲壳素[J].西北林学院学报,2019,34(2):179-186.[doi:10.3969/j.issn.1001-7461.2019.02.27]
　LIU Xin-xu,ZENG Hui,SHI Peng,et al. Bacterium-protease Combined Method to Extract Chitin from Monochamus alternatus[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2019,34(2):179-186.[doi:10.3969/j.issn.1001-7461.2019.02.27]
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 菌酶联合法提取松墨天牛甲壳素()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第34卷
期数:
2019年第2期
页码:
179-186
栏目:
出版日期:
2019-03-31

文章信息/Info

Title:
 Bacterium-protease Combined Method to Extract Chitin from Monochamus alternatus
文章编号:
1001-7461(2019)02-0179-08
作者:
 刘信旭1; 曾辉2; 时鹏1; 马晓光3; 石娟1*
 （1.北京林业大学 林木有害生物防治北京市重点试验室，北京 100083；2.辽宁林业有害生物防治检疫局，辽宁 沈阳 110804；3.北京水光科技有限公司，北京 100052）
Author(s):
 LIU Xin-xu1; ZENG Hui2; SHI Peng1; MA Xiao-guang3; SHI Juan1*
 （1.Key Laboratory of Beijing Municipality for Forest Pest Control,Beijing Forestry University,Beijing 100083,China; 2.Forestry Pest Control and Quarantine Bureau of Liaoning,Shenyang 110804,Liaoning,China; 3.Beijing Shuiguang Technology Co.,Ltd.,Beijing 100052,China）
关键词:
 ; 甲壳素; 菌种发酵; 蛋白质酶解; 松墨天牛
Keywords:
 ; chitin;  bacterium fermentation;  protein enzymolysis;  Monochamus alternatus
分类号:
S718.7
DOI:
10.3969/j.issn.1001-7461.2019.02.27
文献标志码:
A
摘要:
 甲壳素（chitin）是一种重要的可再生资源。松墨天牛（Monochamus alternatus）是松属植物的重要蛀干害虫，其壳质化程度高，是甲壳素的优质资源。以松墨天牛为原料，探讨“菌酶联合法”提取昆虫甲壳素的可行性和应用前景。结果表明：1）联合发酵最佳条件是8%松墨天牛粉末、8%葡萄糖液体培养基，接种11%的凝结芽孢杆菌，恒温摇床温度为50℃，转速为100 r/min，同时加入蛋白酶K和凝结芽孢杆菌发酵5 d后得到发酵产物，最终甲壳素得率为32.51%，产物中甲壳素纯度为72.78%，灰分残留量占甲壳素产品的1.42%，蛋白质残留量占甲壳素产品的25.74%。2）红外光谱鉴定结果表明，通过菌酶联合法制取的甲壳素相比于传统酸碱法提取的甲壳素品质好，具有良好的应用前景。
Abstract:
 Chitin is an important renewable resource.Monochamus alternatus,is an key pest to genus Pinus.Compared to other insects,it has a higher degree of chitinization and a higher content of chitin,it is a high-quality of chitin resource.In view of this,this paper usesd M.alternatus as the raw material to explore the feasibility and application prospect of the extraction of chitin by the “bacterium-protease combined method”.The main findings were as follows:1)The optimum conditions for combined fermentation were 8% M.alternifolius powder,8% glucose liquid medium,inoculation of 11% Bacillus coagulans,constant temperature shaker at 50℃,rotation speed at 100 r/min,and simultaneous addition of proteinase K After fermentation with B.coagulans for 5 days.After being identified as chitin,the final yield of chitin was 32.51%,the purity of the chitin in the product was 72.78%,the residual ash content was 1.42% of the chitin product,and the protein residue was 25.74% of the chitin product.2) The infrared spectrum identification results indicated that the chitin prepared by the bacterium-protease combined method had higher quality than the traditional acid-alkali method extraction,and thus had more advantages and application prospects.

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备注/Memo

备注/Memo:
 收稿日期：2018-05-28修回日期：2018-09-15
基金项目：中央高校基本科研业务费专项资金（2016ZCQ07）；国家自然科学基金面上项目(31770687)。
作者简介：刘信旭，男，在读硕士，研究方向：林木有害生物防治。E-mail：XinxuLiu@foxmail.com
*通信作者：石娟，女，博士生导师，研究方向：林业外来生物入侵、植物检疫。E-mail：shi_juan@263.net
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