[1]任得元,赵焕元,刘玉梅,等. 核桃翻译起始因子JreIF1A的克隆及抗旱功能分析[J].西北林学院学报,2019,34(6):103-108.[doi:10.3969/j.issn.1001-7461.2019.06.16]
 REN De-yuan,ZHAO Huan-yuan,LIU Yu-mei,et al. Identification and Drought Stress Response Analysis of Eukaryotic Initiation Factor JreIF1A from Juglans regia[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2019,34(6):103-108.[doi:10.3969/j.issn.1001-7461.2019.06.16]
点击复制

 核桃翻译起始因子JreIF1A的克隆及抗旱功能分析()
分享到:

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第34卷
期数:
2019年第6期
页码:
103-108
栏目:
出版日期:
2019-11-30

文章信息/Info

Title:
 Identification and Drought Stress Response Analysis of Eukaryotic Initiation Factor JreIF1A from Juglans regia
文章编号:
1001-7461(2019)06-0103-06
作者:
 任得元1赵焕元2刘玉梅2高向倩2孙宇栋2杨桂燕2*
 (1.杨凌职业技术学院,陕西 杨陵 712100;2.西北农林科技大学 林学院,陕西 杨陵 712100)
Author(s):
 REN De-yuan1ZHAO Huan-yuan2LIU Yu-mei2GAO Xiang-qian2SUN Yu-dong2YANG Gui-yan2*
 (1.Yangling Vocational & Technical College,Yangling 712100,Shaanxi China; 2.College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi China)
关键词:
 核桃真核翻译起始因子eIF1A基因逆境响应酵母转化
Keywords:
Juglans regia eukaryotic translation initiation factor eIF1A gene stress response yeast transformation
分类号:
Q943.2
DOI:
10.3969/j.issn.1001-7461.2019.06.16
文献标志码:
A
摘要:
 真核生物翻译起始因子(eIF1)在逆境响应中具有一定的调节功能。本研究克隆获得核桃(Juglans regia)的1条eIF1A基因(命名为JreIF1A),通过生物信息学、基因表达及酵母转化等不同技术,分析JreIF1A在干旱胁迫下的基本生物功能。结果表明,JreIF1A的开放阅读框为438 bp,编码的多肽含145个氨基酸,分子量为16 344.48 u,理论等电点为5.08。JreIF1A与土瓶草(Cephalotus follicularis)、毛果杨(Populus trichocarpa)等的亲缘关系较近。JreIF1A能被干旱胁迫显著诱导,且在根和叶中具有表达特异性。将JreIF1A转入酵母,对转基因酵母INVSC1(pYES2-JreIF1A)及对照酵母INVSC1(pYES2)分别进行干旱(山梨醇,0.2 mol/L)处理,发现INVSC1(pYES2-JreIF1A)在干旱胁迫下的生长活性显著优于INVSC1(pYES2)。表明JreIF1A基因能不同程度地响应干旱胁迫,其表达可有效改善转基因酵母的抗旱响应能力;JreIF1A基因可作为核桃逆境适应机制研究的候选基因。
Abstract:
 The eukaryotic translation initiation factors (eIF1s) play vital roles in plant stress response.In this study,an eIF1A gene (named JreIF1A) was obtained from Juglans regia,and the basic biological functions of JreIF1A under drought stress was analyzed by different techniques containing bioinformatics,gene expression and yeast transformation.The results showed that the open reading frame (ORF) of JreIF1A was 438 bp,the encoded polypeptide contained 145 amino acids,the molecular weight was 16 344.48 u,and the theoretical isoelectric point was 5.08.JreIF1A shared close evolution relationship with the eIF1s from Cephalotus follicularis and Populus trichocarpa.JreIF1A could be significantly induced by drought stress,and had expression specificity in roots and leaves.JreIF1A was transferred into yeast,and transgenic yeast INVSC1(pYES2-JreIF1A) and control yeast INVSC1 (pYES2) were subjected to drought (sorbitol,0.2 mol/L) stress,from which it was found that the growth activity of INVSC1(pYES2-JreIF1A) was significantly better than INVSC1(pYES2) under drought stress.These results indicated that JreIF1A gene could respond to drought stress,and its expression could effectively improve the drought stress response ability of transgenic yeast.JreIF1A gene can be used as a candidate gene for walnut stress adaptation mechanism.

相似文献/References:

[1]董丽芬,邹朋波,贾彩霞,等.核桃微体嫁接方法研究[J].西北林学院学报,2007,22(02):79.
 DONG Li-fen,ZOU Peng-bo,JIA Cai-xia,et al.A Study on Micro-graft Methods of Juglans regia[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(6):79.
[2]王 炜,李鹏霞,梁丽松,等.不同贮藏温度对核桃脂肪酸氧化的影响[J].西北林学院学报,2008,23(06):159.
 WANG Wei,LI Pengxia,LIANG Lisong,et al.Effects of Storage Temperature on Fatty Acid Oxidation of Juglans regi a[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(6):159.
[3]范成民,董丽芬,朱帜,等.核桃芽苗砧嫁接方法研究[J].西北林学院学报,2008,23(04):109.
 FAN Cheng min,DONG Li fen*,ZHU Zhi,et al.Nurse Seed Grafting Methods of Juglans regia[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(6):109.
[4]翟梅枝,高绍棠,王晓斌,等.核桃露地嫁接试验研究[J].西北林学院学报,2000,15(03):41.
 Zhai Meizhi,Gao Shaotang,Wang Xiaobin,et al.A Study on Walnut Grafting Trial in Open-air Condition[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2000,15(6):41.
[5]刘朝斌,高绍棠,黄昌新.哈特勒品种核桃引种调查[J].西北林学院学报,2000,15(03):36.
 Liu Chaobin,Gao Shaotang,Huang Changxin.An Investigation of the Introduction of "Hartley" Walnut Variety[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2000,15(6):36.
[6]李浩波,陈虎明,樊伟,等.影响核桃芽接成活率的制约因子及其有效控制研究[J].西北林学院学报,2002,17(03):22.
 Studies on Defination and Effective Control of Limitative Factors Affecting the Walnut Budding Take[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2002,17(6):22.
[7]史俊燕,樊金拴,任秋芳.核桃子苗嫁接技术研究[J].西北林学院学报,2004,19(01):66.
 A Study on Walnut Seedling Grafting[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2004,19(6):66.
[8]张宏辉.核桃快速嫁接育苗技术研究[J].西北林学院学报,2004,19(02):53.
 Rapid Propagation of Grafting Plant of Juglans regia[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2004,19(6):53.
[9]赵廷松,方文亮,曾清贤.5个核桃早实杂交新品种鲁甸县区域试验[J].西北林学院学报,2007,22(05):83.
 ZHAO Ting-song,FANG Wen-liang,ZENG Qing-xian.Performance of Five Early-fruiting New Hybrids of Walnut in Ludian County[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(6):83.
[10]翟梅枝,问小强,刘枫,等.核桃属植物内生真菌的分离及其抑菌活性研究[J].西北林学院学报,2009,24(03):144.
 ZHAI Mei-zhi,WEN Xiao-qiang,LIU Feng,et al.Isolation of Endophytic Fungi from Walnut and Their Antifungaul Activities[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2009,24(6):144.
[11]陈少瑜,张雨,陈霞,等. 27份核桃种质资源亲缘关系的ISSR分析[J].西北林学院学报,2012,27(04):108.
 CHEN Shao-yu,ZHANG Yu,CHEN Xia,et al. Genetic Relationship of 27 Walnut Germplasm Resources Based on ISSR Markers[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(6):108.
[12]高智辉,翟梅枝,王云果,等. 核桃内生真菌的分离鉴定[J].西北林学院学报,2012,27(05):121.
 GAO Zhi-hui,ZHAI Mei-zhi,WANG Yun-guo,et al. Isolation and Identification of Endophytic Fungi from Walnut Tree[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(6):121.
[13]肖良俊,宁德鲁,彭明俊,等. 滇东北核桃优良单株主要经济性状的主成分分析[J].西北林学院学报,2013,28(02):79.[doi:10.3969/j.issn.1001-7461.2013.02.15]
 XIAO Liang-jun,NING De-lu,PENG Ming-jun,et al. Principal Component Analysis for Major Economic Characteristics of Walnut in Northeastern Yunnan[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(6):79.[doi:10.3969/j.issn.1001-7461.2013.02.15]
[14]王磊,解孝满,李文清*,等. 山东核桃主要分布区种质资源遗传多样性AFLP分析[J].西北林学院学报,2014,29(03):113.[doi:doi:10.3969/j.issn.1001-7461.2014.03.23]
 WANG Lei,XIE Xiao-man,LI Wen-qing*,et al. AFLP Analysis on the Genetic Diversity of Juglans regia Germplasm Resources from Main Distribution Areas in Shandong Province[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(6):113.[doi:doi:10.3969/j.issn.1001-7461.2014.03.23]
[15]樊江斌,张金龙,许新平,等. 陕西2013年核桃晚霜冻害发生情况调查与分析[J].西北林学院学报,2014,29(05):120.[doi:doi:10.3969/j.issn.1001-7461.2014.05.22]
 FAN Jiang-bin,ZHANG Jin-long,XU Xin-ping,et al. An Investigation on Frozen Damage of Walnut Trees in Shaanxi Province[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(6):120.[doi:doi:10.3969/j.issn.1001-7461.2014.05.22]
[16]刘娇,范志远*,赵廷松,等. 鲁甸县抗寒核桃优良无性系选育研究[J].西北林学院学报,2015,30(01):102.[doi:doi:10.3969/j.issn.1001-7461.2015.01.16]
 LIU Jiao,FAN Zhi-yuan*,ZHAO Ting-song,et al. Breeding of Cold Resistant Walnut Superior Clones in Ludian County[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(6):102.[doi:doi:10.3969/j.issn.1001-7461.2015.01.16]
[17]周斌,张金龙,冯纪年,等. 短期低温胁迫对核桃抗氧化酶活性的影响[J].西北林学院学报,2015,30(03):51.[doi:doi:10.3969/j.issn.1001-7461.2015.03.09]
 ZHOU Bin,ZHANG Jin-long,FENG Ji-nian,et al. Antioxidant Enzymes Activities of Walnut Tree under Short Term Cold Stress[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(6):51.[doi:doi:10.3969/j.issn.1001-7461.2015.03.09]
[18]邓拓,张金龙,冯纪年,等. 壳聚糖对低温胁迫下核桃抗冻反应的激发作用[J].西北林学院学报,2016,31(1):60.[doi:10.3969/j.issn.1001-7461.2016.01.11]
 DENG Tuo,ZHANG Jin-long,FENG Ji-nian,et al. Elicitation Effect of Chitosan on Cold Tolerance of Walnut Tree Under Cold Stress[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(6):60.[doi:10.3969/j.issn.1001-7461.2016.01.11]
[19]粟莉圆,李大培,常远,等. 核桃JrGSTTau1基因的克隆及转入酵母的抗逆功能分析[J].西北林学院学报,2016,31(3):98.[doi:10.3969/j.issn.1001-7461.2016.03.16]
 SU Li-yuan,LI Da-pei,CHANG Yuan,et al.Cloning and Stress Tolerance Analysis of a JrGSTTau1 Gene from Juglans regia in Saccharomyces cerevisiae[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(6):98.[doi:10.3969/j.issn.1001-7461.2016.03.16]
[20]阿依佳玛丽·依玛尔,刘泽星,毛光瑞,等. 不同生境春季核桃内生真菌分离及其多样性[J].西北林学院学报,2016,31(6):184.[doi:10.3969/j.issn.1001-7461.2016.06.32]
 YIMAER·A-yi-jia-ma-li,LIU Ze-xing,MAO Guang-rui,et al. Isolation and Diversity of Endophytic Fungi from Juglans regia in Spring under Different Habitats[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(6):184.[doi:10.3969/j.issn.1001-7461.2016.06.32]

备注/Memo

备注/Memo:
 收稿日期:2019-03-26修回日期:2019-06-02
基金项目:中央财政林业科技推广项目“千阳核桃综合配套丰产栽培技术示范与推广”(SLTG[2016]14号);陕西省自然科学基础研究计划项目(2018JQ3066)。
作者简介:任得元,男,高级工程师,研究方向:林业与技术推广。E-mail:Rdy123@163.com
*通信作者:杨桂燕,女,讲师,研究方向:林木遗传育种。E-mail:yang861026@163.com
更新日期/Last Update: