[1]陈钢,母天燕,曹光球,等. 杉木蔗糖转运蛋白SUT基因的克隆和功能分析[J].西北林学院学报,2020,35(2):8-14.[doi:10.3969/j.issn.1001-7461.2020.02.02]
　CHEN Gang,MU Tian-yan,CAO Guang-qiu,et al. Cloning and Functional Analysis of Chinese Fir Sucrose Transporter SUT Gene[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(2):8-14.[doi:10.3969/j.issn.1001-7461.2020.02.02]
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 杉木蔗糖转运蛋白SUT基因的克隆和功能分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第35卷
期数:
2020年第2期
页码:
8-14
栏目:
出版日期:
2020-03-31

文章信息/Info

Title:
 Cloning and Functional Analysis of Chinese Fir Sucrose Transporter SUT Gene
文章编号:
1001-7461(2020)02-0008-07
作者:
 陈钢1; 2; 母天燕1; 2; 曹光球1; 2; 林强3; 郑宏3; 曹世江1; 2*
 (1.福建农林大学 林学院，福建 福州 350002；2.国家林业和草业局 杉木工程技术研究中心，福建 福州 350002；3.福建省洋口国有林场，福建 顺昌 353211)
Author(s):
 CHEN Gang1; 2; MU Tian-yan1; 2; CAO Guang-qiu1; 2; LIN Qiang3; ZHENG Hong3; CAO Shi-jiang1; 2*
 (1.College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China; 2.Chinese Fir Engineering Technology Research Center,National Forestry and Grassland Administration,Fuzhou 350002,Fujian,China; 3.Yangkou State-owned Forest Farm,Shunchang 353211,Fujian,China)
关键词:
 ; 蔗糖转运蛋白; 基因克隆; 功能分析; 杉木
Keywords:
 ; sucrose transporter;  gene cloning;  functional analysis;  Chinese fir
分类号:
S791.27
DOI:
10.3969/j.issn.1001-7461.2020.02.02
文献标志码:
A
摘要:
 蔗糖转运蛋白又称蔗糖-质子共转运蛋白，简称SUT(sucrose transporter)或者SUC(sucrose carriers)，主要担负植物光合产物——蔗糖的跨膜运输与分配调控，在植物体内完成使蔗糖从“源”到“库”的运输。采用CTAB法提取杉木总RNA，通过RT-PCR得到cDNA，设计基因特异性引物，从1年生无性系杉木幼苗嫩叶中克隆SUT基因，并利用生物信息学方法分析SUT的基本结构、进化关系、理化性质。结果表明，在电泳图上总RNA的结构完整，28 S的亮度约为18 S的2倍；A260/A280为2.20，A260/A230为2.31；杉木SUT基因编码区全长为1 782 bp，共编码593个氨基酸，是具有SUT基因家族的典型12个跨膜区；通过系统进化树的分析发现，杉木SUT与无油樟SUT的亲缘性较高，SUT蛋白属于疏水性蛋白。
Abstract:
 The sucrose transporter,also known as the sucrose-proton cotransporter,abbreviated as SUT (sucrose transporter) or SUC (sucrose carriers) is mainly responsible for the transmembrane transport and distribution regulation of plant photosynthetic product-sucrose.It helps the transportation of sucrose from “source” to “library” in plants.In this study,the total RNA of Chinese fir was extracted by CTAB method.The cDNA was obtained by RT-PCR,and the gene-specific primers were designed.The SUT gene was cloned from the young leaves of annual clones of Chinese fir.The basic structure,evolution,and physico-chemical properties of SUT were analyzed by bioinformatics.The electropherogram indicated that the structure of total RNA was complete,the brightness of 28 S was about twice that of 18 S; A260/A280 was 2.20,A260/A230 was 2.31; and the length of Chinese SUT gene coding region was 1 782 bp.It encoded 593 amino acids and was a typical 12 transmembrane region with SUT gene family.Through phylogenetic tree analysis,it was found that Chinese fir SUT had higher affinity with oil-free SUT,and SUT protein was hydrophobic.

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备注/Memo

备注/Memo:
 收稿日期：2019-06-20修回日期：2019-09-13
基金项目：福建农林大学林学高峰学科建设项目（71201800739）；大学生创新项目（118-111ZC7074）。
作者简介：陈钢，女，硕士在读，研究方向：植物地理学。E-mail:goodluckgirl2017@outlook.com
*通信作者：曹世江，男，博士，讲师，研究方向：林木遗传育种。E-mail:csjiang1123 @126.com
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