[1]高壮壮,娄倩,刘雅莉*. 蝴蝶兰‘大辣椒’组培快繁技术体系的优化[J].西北林学院学报,2020,35(4):95-100.[doi:10.3969/j.issn.1001-7461.2020.04.16]
 GAO Zhuang-zhuang,LOU Qian,LIU Ya-li*. Optimization of the Tissue Culture and Rapid Propagation of Phalaenopsis Cultivar ‘Big Chili’[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(4):95-100.[doi:10.3969/j.issn.1001-7461.2020.04.16]
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 蝴蝶兰‘大辣椒’组培快繁技术体系的优化()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第35卷
期数:
2020年第4期
页码:
95-100
栏目:
出版日期:
2020-09-29

文章信息/Info

Title:
 Optimization of the Tissue Culture and Rapid Propagation of Phalaenopsis Cultivar ‘Big Chili’
文章编号:
1001-7461(2020)04-0095-06
作者:
 高壮壮娄倩刘雅莉*
 (西北农林科技大学 旱区作物逆境生物学家重点实验室,农业部西北地区园艺作物生物学与种质创制重点实验室,陕西 杨陵 712100)
Author(s):
 GAO Zhuang-zhuangLOU QianLIU Ya-li*
 (State Key Laboratory of Crop Stress Biology in Arid Areas,Ministry of Agriculture Key Labooratory of Horticultural Plant Biology and Germplasm Innovation,Northwest A&F University,Yangling 712100,Shaanxi,China)
关键词:
 蝴蝶兰不定芽增殖生根快速繁殖
Keywords:
Phalaenopsis aphrodite adventitious bud proliferation rooting rapid propagation
分类号:
S682.31
DOI:
10.3969/j.issn.1001-7461.2020.04.16
文献标志码:
A
摘要:
 探索优化蝴蝶兰组培快繁技术体系,以蝴蝶兰‘大辣椒’半木质化花梗为试验材料,研究消毒剂、基础培养基、植物生长调节剂配比及浓度对蝴蝶兰不定芽诱导、增殖和生根过程的影响。结果表明:1)先用2% H2O2溶液处理15 min,再用10% NaClO溶液处理10 min,消毒效果良好,花梗污染率低,为26.6%;2)不定芽诱导最适培养基为花宝1号+花宝2号+ 6-BA 3 mg/L+NAA 0.05 mg/L,出芽率为78.9%;3)不定芽增殖最适培养基为花宝1号+6-BA 5 mg/L+NAA 0.05 mg/L,增殖系数为3.60;4)幼苗生根最适培养基为1/2MS+NAA 0.2mg/L,生根率为73.7%。本研究旨在优化‘大辣椒’品种的组培快繁技术体系,为进一步建立蝴蝶兰再生体系和工厂化生产提供技术支持。
Abstract:
 In order to develop and optimize the technique system of tissue culture and rapid propagation of Phalaenopsis aphrodite,semi-lignified peduncles of a P.aphrodite cultivar ‘Big Chili’ were used as test materials to study the effects of disinfectant,basic medium,plant growth regulator on the induction and proliferation of adventitious buds,proliferation,and rooting process.The results showed that 1) the infection rate of peduncles was low (26.6%) after they were treated by 2% H2O2 for 10 min and 10% NaClO for 15 min; 2) The optimal medium for adventitious bud induction was Hyponex No.1+Hyponex No.2+6-BA 3 mg/L+NAA 0.05 mg/L,the germination rate was 78.9%; 3) The optimal medium for adventitious bud proliferation was Hyponex No.1+6-BA 5 mg/L+NAA 0.05 mg/L,the multiplication coefficient was 3.60; 4) The optimum medium for rooting was 1/2MS+NAA 0.2 mg/L,the rooting rate was 73.7%.The results of this study could be used as the technical support for the rapid propagation of P.aphrodite in large scale.

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备注/Memo

备注/Memo:
 收稿日期:2020-03-20修回日期:2020-05-02
基金项目:国家自然科学基金(31471905,31701962)。
作者简介:高壮壮。研究方向:园林植物遗传育种。E-mail:Gaoz4207@163.com
*通信作者:刘雅莉,硕士,博士生导师。研究方向:园林植物遗传育种。E-mail:lyl6151@126.com
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