[1]程玮哲,樊军锋*,周永学,等. 基于荧光SSR标记的10个白杨派种质资源遗传多样性分析[J].西北林学院学报,2021,36(3):88-93.[doi:10.3969/j.issn.1001-7461.2021.03.13]
　CHENG Wei-zhe,FAN Jun-feng*,ZHOU Yong-xue,et al. Genetic Diversity Analysis of the 10 Poplar Accessions Based on SSR Molecular Makers[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2021,36(3):88-93.[doi:10.3969/j.issn.1001-7461.2021.03.13]
点击复制

 基于荧光SSR标记的10个白杨派种质资源遗传多样性分析()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第36卷
期数:
2021年第3期
页码:
88-93
栏目:
出版日期:
2021-05-31

文章信息/Info

Title:
 Genetic Diversity Analysis of the 10 Poplar Accessions Based on SSR Molecular Makers
文章编号:
1001-7461(2021)03-0088-06
作者:
 程玮哲; 樊军锋*; 周永学; 高建社; 俞永玮; 王烟霞
 （西北农林科技大学 林学院，陕西 杨陵 712100）
Author(s):
 CHENG Wei-zhe; FAN Jun-feng*; ZHOU Yong-xue; GAO Jian-she; YU Yong-wei; WANG Yan-xia
 （College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China)
关键词:
 ; 白杨派; SSR; 毛细管电泳; 指纹图谱; 遗传分析
Keywords:
 ; Sect.Leuce;  SSR;  capillary electrophoresis;  fingerprinting;  genetic analysis
分类号:
S792.11
DOI:
10.3969/j.issn.1001-7461.2021.03.13
文献标志码:
A
摘要:
 为分析白杨派优良无性系之间的遗传关系，采用毛细管电泳荧光SSR分子标记技术，构建10个白杨派优良无性系DNA指纹图谱，并进行遗传分析。结果表明，从20对引物中筛选出7对位点多态性高且重复性好的引物，共扩增出41个多态性位点，平均每对引物扩增5.9个，每个引物扩增多态位点在4~8之间。无性系间遗传相似系数区间为0.415~0.902，平均遗传相似系数为0.657，遗传变异性丰富。Shannon指数和基因多样性指数分别为1.433 4和0.694 3，表明无性系间具有较高的遗传多样性。聚类分析表明，10个白杨派无性系中，秦白杨1号、秦白杨3号、秦白杨5号与84K聚为一类，07-17-18、07-23-23与07-30-11与I-101聚为一类，与实际亲缘关系相符。利用2对多态性SSR引物PMGC-2020和PMGC2607构建了白杨派无性系指纹图谱，该图谱可将无性系全部区分开。
Abstract:
 To analyze the genetic relationships of poplar clones (Sect.Leuce),we constructed DNA fingerprint maps of 10 excellent poplar clones and analyzed genetic diversity based on fluorescent SSR molecular markers of capillary electrophoresis.A total of 41 polymorphic loci were detected in the 7 pairs of polymorphic primers with high polymorphism and high repeatability,20 pairs of primers were screened out.The polymorphic loci of each primer ranged from 4 to 8 with an average of 5.9.The genetic similarity coefficient ranged from 0.415 to 0.902,with the average of 0.657.The Shannon index and gene diversity index were 1.433 4 and 0.694 3,respectively,indicating that there was a high genetic diversity.Cluster analysis showed that “Qinbaiyang No.1”,“Qinbaiyang No.3”,“Qinbaiyang No.5” and “84K” were clustered in one group,and “07-17-18”,“07-23-23”,“07-30-11” and “I-101” were clustered another group,which was consistent with the actual genetic relationship.All clones could be distinguished by the fingerprints built by the primers of PMGC-2020 and PMGC2607.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(3):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(3):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(3):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
[11]曹佳乐,延娜,樊军锋*,等. 4个白杨派新无性系抗寒性鉴定和综合评价[J].西北林学院学报,2016,31(2):130.[doi:10.3969/j.issn.1001-7461.2016.02.23]
　CAO Jia-le,YAN Na,FAN Jun-feng*,et al. Determination and Comprehensive Evaluation on Cold-tolerance of Four New Populus Clones[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(3):130.[doi:10.3969/j.issn.1001-7461.2016.02.23]
[12]郑涛,樊军锋*,高建社,等. 11个白杨派无性系抗旱性综合评价[J].西北林学院学报,2019,34(4):101.[doi:10.3969/j.issn.1001-7461.2019.04.15]
　ZHENG Tao,FAN Jun-feng*,GAO Jian-she,et al. On the Comprehensive Evaluation of Drought Resistance of 11 Poplar Clones[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2019,34(3):101.[doi:10.3969/j.issn.1001-7461.2019.04.15]
[13]张海燕,樊军锋*,周永学,等. 8个白杨无性系抗寒性测定与评价[J].西北林学院学报,2020,35(2):87.[doi:10.3969/j.issn.1001-7461.2020.02.12]
　ZHANG Hai-yan,FAN Jun-feng*,ZHOU Yong-xue,et al. Determination and Evaluation on Cold-resistance of 8 Poplar Clones[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(3):87.[doi:10.3969/j.issn.1001-7461.2020.02.12]
[14]许兴华,李善文*,潘瞳,等. 白杨派优良无性系选育研究[J].西北林学院学报,2023,38(3):87.[doi:10.3969/j.issn.1001-7461.2023.03.12]
　XU Xing-hua,LI Shan-wen*,PAN Tong,et al. A Study on Breeding of Fine Clones in Leuce[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2023,38(3):87.[doi:10.3969/j.issn.1001-7461.2023.03.12]

备注/Memo

备注/Memo:
 收稿日期：2020-09-11修回日期：2020-10-20
基金项目：“十三五”国家重点研发项目（SQ2016YFNC030041）。
作者简介：程玮哲。研究方向：林木遗传育种与林业生物技术。E-mail：412022317@qq.com
*通信作者：樊军锋，博士，教授。研究方向：杨树新品种选育与遗传改良。E-mail：fanjf282163.com
更新日期/Last Update: