[1]杨乐,张康,李龙*. 毛竹AUX1/LAX候选基因家族鉴定与表达分析[J].西北林学院学报,2022,37(1):89-95.[doi:10.3969/j.issn.1001-7461.2022.01.13]
　YANG Le,ZHANG Kang,LI Long*. Identification and Expression Analysis of AUX1/LAX Gene Family in Moso Bamboo (Phyllostachys edulis)[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2022,37(1):89-95.[doi:10.3969/j.issn.1001-7461.2022.01.13]
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 毛竹AUX1/LAX候选基因家族鉴定与表达分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第37卷
期数:
2022年第1期
页码:
89-95
栏目:
出版日期:
2022-01-31

文章信息/Info

Title:
 Identification and Expression Analysis of AUX1/LAX Gene Family in Moso Bamboo (Phyllostachys edulis)
文章编号:
1001-7461(2022)01-0089-07
作者:
 杨乐; 张康; 李龙*
 （西北农林科技大学 林学院，陕西 杨陵 712100）
Author(s):
 YANG Le; ZHANG Kang; LI Long*
 (College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China)
关键词:
 ; AUX1/LAX; 毛竹; 基因表达; 生物信息学
Keywords:
 ; AUX1/LAX;  moso bamboo;  gene expression;  bioinfomatics
分类号:
S718.46
DOI:
10.3969/j.issn.1001-7461.2022.01.13
文献标志码:
A
摘要:
 AUX1/LAX家族是一类生长素输入载体，在植物生长发育和形态建成过程中起着重要的调控作用。利用同源比对的方法从毛竹基因组中鉴定出8个PheAUX1/LAX候选基因，进行系统进化树、基因结构、基序分析。结果表明，PheAUX1/LAX候选基因分为Class Ⅰ和Class Ⅱ 2个亚组，其中classⅠ的候选基因包含6~7个外显子，而ClassⅡ的候选基因包含8~10个外显子。不同组织器官转录组数据分析表明多数Class Ⅰ候选成员在毛竹冬笋中高丰度表达，而Class Ⅱ中的PH02Gene38722.t在跳鞭中高丰度表达。通过qRT-PCR对AUX1/LAX候选基因在不同生长发育时期笋中的表达量分析表明，有6个候选基因与对照相比呈现出不同程度的上调表达，表明PheAUX1/LAX候选基因广泛参与毛竹笋的生长发育。本研究将为进一步研究毛竹AUX1/LAX基因的功能和调控机制奠定理论基础。
Abstract:
 AUX1/LAX family members are the major auxin influx carriers,which are widely participated in plant growth and development.However,little information is available about the AUX1/LAX family in moso bamboo (Phyllostachys edulis).In this study,a total of 8 AUX1/LAX genes were identified in Moso bamboo genome.According to gene structure,conserved motif and phylogenetic relationships,the PheAUX1/LAXs were classified into two classes.The expression patterns of AUX1/LAXs based on transcriptome sequencing data indicated that four AUX1/LAX genes belonged to class Ⅰ exhibited high expression levels in winter bamboo shoot.The PH02Gene38722.t1-a class Ⅱ member showed high expression level in outward-rhizome.Expression analysis based on qRT-PCR indicated that six AUX1/LAXs showed high accumulation level during moso bamboo shoot growth,indicating the significant importance of AUX1/LAXs during moso bamboo shoot growth.Our study can provide a theoretical foundation and some experimental evidence for further functional verification of the PheAUX1/LAX.

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备注/Memo

备注/Memo:
 收稿日期：2021-01-20修回日期：2021-04-28
基金项目：陕西省青年科学基金（2019JQ-402）；中国博士后基金（2018M633594）。
第一作者：杨乐。研究方向：林木遗传育种。E-mail：yanglenwafu@163.com
*通信作者：李龙，讲师。研究方向：林木遗传育种。E-mail：lilong1949@126.com
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