[1]王翠兰,左璐莹,张绍勇,等. 7种杀菌剂和5种植物抗病激活剂对油茶炭疽病病菌活性研究[J].西北林学院学报,2022,37(2):176-179.[doi:10.3969/j.issn.1001-7461.2022.02.24]
　WANG Cui-lan,ZUO Lu-ying,ZHANG Shao-yong,et al. Activities of 7 Fungicides and 5 Plant Disase Elicitors on the Pathogen of Camellia oleifera[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2022,37(2):176-179.[doi:10.3969/j.issn.1001-7461.2022.02.24]
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 7种杀菌剂和5种植物抗病激活剂对油茶炭疽病病菌活性研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第37卷
期数:
2022年第2期
页码:
176-179
栏目:
出版日期:
2022-03-31

文章信息/Info

Title:
 Activities of 7 Fungicides and 5 Plant Disase Elicitors on the Pathogen of Camellia oleifera
文章编号:
1001-7461(2022)02-0176-04
作者:
 王翠兰1; 左璐莹1; 张绍勇2; 陈安良1*
 （1.浙江农林大学 林业与生物技术学院，浙江 杭州 311300；2.湖州师范学院 生命科学学院，浙江 湖州 313000）
Author(s):
 WANG Cui-lan1; ZUO Lu-ying1; ZHANG Shao-yong2; CHEN An-liang1*
 (1.College of Forestry and Biotechnology,Zhejiang Agriculture and Forest University,Hangzhou 311300,Zhejiang,China; 2.School of Life Sciences,Huzhou Teachers College,Huzhou 313000,Zhejiang,China)
关键词:
 ; 油茶; 胶孢炭疽病; 植物抗病激活剂; 抗性诱导
Keywords:
Camellia oleifera;  Colletotrichum gloeosporioides;  plant disase elicitor;  resistance induction
分类号:
S763.306
DOI:
10.3969/j.issn.1001-7461.2022.02.24
文献标志码:
A
摘要:
 胶孢炭疽菌引起的油茶炭疽病是油茶的主要病害之一，试验通过室内毒力测定及活体测定，分别筛选出防治油茶炭疽病的高效杀菌剂和植物抗病激活剂。采用菌丝抑制法测定杀菌剂及植物抗病激活剂对胶孢炭疽菌的毒力；采用离体叶片法，通过病斑减小率评价植物抗病激活剂对油茶炭疽病的诱抗效果。供试7种杀菌剂中以咪鲜胺抑菌活性最强，EC50为0.129 mg/L；嘧菌酯抑菌活性最低，EC50为52.110 mg/L；植物抗病激活剂对菌丝生长无直接抑制作用，但在2年生的油茶苗上喷施后，对病斑在叶片上的扩展均有一定抑制作用，以水杨酸100 mg/L剂量处理的叶片诱导抗病性最佳，诱抗效果为50.13%，芸苔素内酯、茉莉酸甲酯，井岗霉素，苯并噻二唑处理组的最大诱导防病效果依次为36.82%、26.93%、19.67%、17.73%。该试验结果为杀菌剂和植物抗病激活剂的复配制剂提供参考依据，为油茶炭疽病的防治提供新思路。
Abstract:
Camellia anthracnose caused by Colletotrichum gloeosporioides is one of the main diseases of Camellia oleifera.Through indoor virulence test,a fungicide and a plant disease-resistant activator that could effectively control camellia anthracnose were screened out.The virulence of fungicides and plant disease resistance activators to C.gloeosporioides was measured by the mycelial inhibition method; The in vitro leaf method was used to evaluate the induction of plant disease resistance activators on camellia anthracnose by the reduction rate of disease spots anti-defense effect.Among the seven fungicides evaluated,prochloraz had the strongest antibacterial activity,with an EC50 value of 0.129 mg/L; azoxystrobin had the lowest antibacterial activity,with an EC50 value of 52.110 mg/L; the plant disease-resistant activator had the greatest effect on mycelium.There was no direct inhibitory effect on mycelial growth.However,after spraying on two-year-old C.oleifera seedlings,it had a certain inhibitory effect on the spread of diseased spots on the leaves.Treatment of the leaves with salicylic acid with 100 mg/L dose induced the best disease resistance.The disease prevention effect was 50.13%,and the maximum induced disease prevention effects of the brassinolide,methyl jasmonate,Jinggangmycin,and benzothiadiazole treatment groups were 36.82%,26.93%,19.67%,and 17.73%,respectively.The results of this experiment provide a reference for the compound preparation of disease activator and new ideas for the prevention and treatment of C.anthracnose.

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备注/Memo

备注/Memo:
 收稿日期：2021-03-22修回日期：2021-05-12
基金项目：浙江省科技计划项目（2019C02024）。
第一作者：王翠兰，硕士。研究方向:生物农药。E-mail：2500761306@qq.com
*通信作者：陈安良，博士。研究方向:生物农药。E-mail：anlchen@126.com
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