[1]令狐高曼,季晓莲,段榕怡,等. 珙桐体细胞胚胎发生技术研究[J].西北林学院学报,2024,39(2):70-78.[doi:10.3969/j.issn.1001-7461.2024.02.09]
　LINGHU Gao-man,JI Xiao-lian,DUAN Rong-yi,et al. Somatic Embryogenesis in Davidia involucrata[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2024,39(2):70-78.[doi:10.3969/j.issn.1001-7461.2024.02.09]
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 珙桐体细胞胚胎发生技术研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第39卷
期数:
2024年第2期
页码:
70-78
栏目:
出版日期:
2024-03-31

文章信息/Info

Title:
 Somatic Embryogenesis in Davidia involucrata
文章编号:
1001-7461(2024)02-0070-09
作者:
 令狐高曼1; 季晓莲2; 段榕怡1; 刘睿1; 戴钊1; 董嘉明1; 徐金颢1; 康永祥1*
 （1.西北农林科技大学 林学院，陕西 杨陵 712100；2.杨凌职业技术学院，陕西 杨陵 712100）
Author(s):
 LINGHU Gao-man1; JI Xiao-lian2; DUAN Rong-yi1; LIU Rui1; DAI Zhao1; DONG Jia-ming1; XU Jin-hao1; KANG Yong-xiang1*
 (1.College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China; 2.Yangling Vocational and Technical College,Yangling 712100,Shaanxi,China)
关键词:
 ; 珙桐; 胚性愈伤组织; 体细胞胚; 植株再生; 组织细胞学
Keywords:
Davidia involucrata;  embryogenic callus;  somatic embryos;  plantlet regeneration;  histocytology
分类号:
S792.99
DOI:
10.3969/j.issn.1001-7461.2024.02.09
文献标志码:
A
摘要:
 研究探讨基本培养基类型对珙桐愈伤组织诱导的影响、植物生长调节剂对胚性愈伤组织诱导及体胚发育的影响，初步建立以未成熟合子胚为外植体，通过体细胞胚胎发生途径再生珙桐植株的培养方案。结果表明，在含有3%蔗糖、0.3%凝胶、800 mg/L水解酪蛋白、400 mg/L L-谷氨酰胺的MS、1/2MS和WPM基本培养基上均可诱导出愈伤组织，以MS+2.0 mg/L 2,4-D+0.5 mg/L 6-BA诱导率最高，可达94.0%；胚性愈伤组织在MS+0.5 mg/L NAA+1.0 mg/L 6-BA的培养基上诱导效果较好；在MS+0.5 mg/L NAA+1.0 mg/L 6-BA培养基上，体胚诱导率可达22.9%；成熟体胚在1/2MS+0.5 mg/L 6-BA+0.25 mg/L IBA的萌发培养基上能正常萌发，其后在添加1 g/L活性炭的萌发培养基上转化成完整小植株。组织细胞学观察表明，珙桐体胚起源于胚性愈伤组织，经历了球形期、心形期、鱼雷期和子叶期发育阶段，这与自然条件下的合子胚形成过程相似。
Abstract:
 The effects of basic medium types on the callus induction of Davidia involucrata and plant growth regulators on embryogenic callus induction and somatic embryo development were studied.A culture scheme for the regeneration of D.involucrata plants by somatic embryogenesis with immature zygotic embryos as explants was preliminarily established.The results showed that callus could be induced on MS,1/2MS and WPM basic media containing 3% sucrose,0.3% gel,800 mg/L hydrolyzed casein,400 mg/L L-glutamine,and MS+2.0 mg/L 2,4-D+0.5 mg/L 6-BA had the highest induction rate of 94.0%.The embryogenic callus was induced on MS+0.5 mg/L NAA+1.0 mg/L 6-BA medium.On MS+0.5 mg/L NAA+1.0 mg/L 6-BA medium,the induction rate of somatic embryos reached 22.9%.Mature somatic embryos could germinate normally on the germination medium of 1/2MS+0.5 mg/L 6-BA+0.25 mg/L IBA,and then transformed into complete plantlets on the germination medium supplemented with 1 g/L activated carbon.Histocytological observation showed that the somatic embryos originated from embryogenic callus and underwent the development stages of globular-,heart-,torpedo- and cotyledonary-stage,which was similar to the formation process of zygotic embryos under natural conditions.

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备注/Memo

备注/Memo:
 收稿日期：2023-01-29修回日期：2023-03-02
基金项目：秦岭珍稀植物红豆杉、珙桐高效繁育技术研究与示范（SXLK2021-01-03）。
第一作者：令狐高曼。研究方向：森林培育学。E-mail：879066569@qq.com
*通信作者：康永祥，博士，教授。研究方向：森林培育学。E-mail：yxkang@nwsuaf.edu.cn
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