[1]王冬梅,宋旭辉,李娟丽,等.卷叶黄精多糖提取分离工艺研究[J].西北林学院学报,2006,21(06):158.
　WANG Dongmei*,SONG Xuhui,LI Juanli,et al.Extraction and Isolation Technology of Polysaccharides from Polygonatum cirrhifolium Root[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(06):158.
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卷叶黄精多糖提取分离工艺研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第21卷
期数:
2006年06期
页码:
158
栏目:
林产化学
出版日期:
2006-12-30

文章信息/Info

Title:
Extraction and Isolation Technology of Polysaccharides from Polygonatum cirrhifolium Root
作者:
王冬梅1; 宋旭辉2; 李娟丽1; 孙世国3
1.西北农林科技大学 林学院，陕西 杨陵 712100; 2.上宋中学，陕西 扶风 722205;3.新疆阿克苏地区林业科学研究所，新疆 阿克苏 843000
Author(s):
WANG Dongmei1*; SONG Xuhui2 ; LI Juanli1 ; SUN Shiguo3
1.College of Forestry, Northwest A  F University, Yangling, Shaanxi 712100, China;2. Shangsong Middle School, Fufeng , Shaanxi 722205, China;3.Akesu Research Institute of Forestry,Akesu,Xinjiang 843000,China
关键词:
卷叶黄精; 多糖;  提取分离工艺
Keywords:
Ploygonatum cirrhifolium;  polysaccharide ;  extraction and isolation technology
分类号:
S759.82
文献标志码:
A
摘要:
采用单因素试验和正交试验对秦岭卷叶黄精（Ploygonatum cirrhifolium）多糖的水提取工艺和醇沉分离工艺进行了研究。结果表明（1）各因素对卷叶黄精多糖的提取率影响是不均等的，影响程度依次为温度＞料液比＞提取时间。最佳提取工艺为在温度80℃下，水提取2次，每次2 h，料液比为1∶25。（2）各因素对醇沉分离效果的影响程度依次为乙醇浓度＞提取液浓缩程度＞pH值。醇沉分离工艺为醇沉时乙醇体积分数为80%，药液浓缩至1 mL·g-1，pH为6。
Abstract:
The extraction and isolation technology of polyscacharides were studied with orthogonal test from Polygonatum oirrhifolium root. The results were as follows(1) The various factors had different influences on the extraction,the sequences of influence was temperature＞ratio of solvent to material＞time. The optimal conditions of extraction were temperature 80℃, solvent water, time2 h;times of extraction2;ratio of solvent to material 1∶25 . (2)The favourable technological parameters for isolating pdysaccarides with ethanol precipitating were when the alcohol consistency came up to 80% in the polysaccharide solution(v/v),the polysaccharides precipitated out more than under other alcohol consistency. The concentration of sample solution is 1 mL· g-1 (material ), pH6.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：20060323修回日期：20060428基金项目：西北农林科技大学科研专项基金（04 ZM027)。作者简介：王冬梅（1970)，女，陕西西安人，讲师，在读博士研究生，主要从事天然产物化学的教学科研工作。
更新日期/Last Update: 2010-05-04