[1]陈彩霞,司瑞新,沈昕,等.转Na+/H+逆向转运蛋白(PeNhaD1)基因派间杂种110杨的获得[J].西北林学院学报,2009,24(01):61-65.
 CHEN Caixia,SI Ruixin,SHEN Xin,et al.Genetic Transformation of PeNhaD1 Gene to the Hybrid Poplar 110[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2009,24(01):61-65.
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转Na+/H+逆向转运蛋白(PeNhaD1)基因派间杂种110杨的获得()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第24卷
期数:
2009年01期
页码:
61-65
栏目:
出版日期:
2009-01-20

文章信息/Info

Title:
Genetic Transformation of PeNhaD1 Gene to the Hybrid Poplar 110
作者:
陈彩霞12 司瑞新2 沈昕1 陈少良1* 张华1 李静1
(1.北京林业大学 生物科学与技术学院, 北京 100083; 2.北京市大东流苗圃,北京 102211)
Author(s):
CHEN Caixia12 SI Ruixin2 SHEN Xin1 CHEN Shaoliang1 ZHANG Hua1 LI Jing1
(1.College of Biological Sciences and Technology, Beijing Forestry University, Beijing 10008, China;2.Beijing Dadongliu Nursery,Beijing 102211, China)
关键词:
PeNhaD1基因基因转化派间杂种110杨
Keywords:
PeNhaD1 gene transformation P. deltoides× P. maximowiczii ‘Eridano’
分类号:
S722.36
文献标志码:
A
摘要:
胡杨Na+/H+逆向转运蛋白基因(PeNhaD1)编码一种质膜Na+/H+逆向转运蛋白,在胡杨细胞拒盐机制中具有重要作用。本研究利用根癌农杆菌(Agrobacterium tumefacience) 介导的叶盘法将PeNhaD1基因转入盐敏感且速生的派间杂种110杨 (P. deltoides× P. maximowiczi ‘Eridano’)中。经PCR和 PCRSouthern 检测已获得转PeNhaD1基因的110杨再生植株。西北林学院学报24卷
Abstract:
The PeNhaD1 gene isolated from Populus euphratica, encoded a plasma Na+/H+ antiporter protein has an important value in the salttolerant mechanism of P.euphratica. In this research, PeNhaD1 gene was transformed to the saltsensitive and fastgrowing hybrid poplar 110 (P. deltoides× P. maximowiczii cv. ‘Eridano’) via Agrobacterium tumefacience mediated transformation system. The result of PCR amplification and PCRSouthern hybridization showed that PeNhaD1 gene had been integrated into the genome of the transgenic hybrid poplar 110 plants.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:20080319修回日期:20080610基金项目:国家自然科学基金重点项目(30430430);863计划项目(2006AA10Z131);全国高校优秀博士学位论文作者专项基金(200152)和教育部高等学校优秀青年教师教学科研奖励计划(2002-323)。作者简介:陈彩霞,女,高级工程师,主要研究方向:植物生物技术。通讯作者:陈少良,教授,博导,主要研究方向:树木抗性生理。Email:lschen@bifu.edu.cn.
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