[1]高海波,沈应柏*.瞬时渗透胁迫对沙冬青细胞离子流的影响[J].西北林学院学报,2011,26(06):35-39.
　GAO Hai bo,SHEN Ying bai.Effect of Sudden Osmotic Stress on Ion Flux in Ammopiptanthus mongolicus Suspension Cells[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2011,26(06):35-39.
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瞬时渗透胁迫对沙冬青细胞离子流的影响
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第26卷
期数:
2011年06期
页码:
35-39
栏目:
森林生物学
出版日期:
2011-11-30

文章信息/Info

Title:
Effect of Sudden Osmotic Stress on Ion Flux in Ammopiptanthus mongolicus Suspension Cells
作者:
高海波1; 2; 沈应柏1*
1.北京林业大学 生命科学与技术学院，北京 100083；2.临沂大学 生命科学学院，山东 临沂 267002
Author(s):
GAO Haibo1; 2; SHEN Yingbai1
1.College of Biological Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，China； 2.College of Life Science，Linyi University，Linyi，Shandong 267002，China
关键词:
沙冬青; 非损伤微测技术; 离子流
Keywords:
Ammopiptanthus mongolicus; noninvasive microtest technique; ion flux
分类号:
S793.9; Q942.5
文献标志码:
A
摘要:
采用非损伤微测技术，研究了沙冬青悬浮细胞经不同浓度的氯化钠、甘露醇处理后，细胞Ca2+、K+、H+3种离子流速、流向及流量的变化。结果表明：氯化钠与甘露醇处理均诱导Ca2+外流，氯化钠诱导的Ca2+外流与氯化钠浓度有关；氯化钠诱导K+外流，而甘露醇诱导K+内流；氯化钠诱导的H+流具有先内流后外流的两个阶段的特点。H+ATP酶在氯化钠诱导的H+外流中具有重要作用。
Abstract:
Ion fluxes of Ca2+, K+ and H+ were studeied in Ammopiptanthus mongolicus suspension cells in response to the treatments of NaCl and mannitol with different concentrations under laboratory conditions by using noninvasive microtest technique. The results showed that Ca2+ flux was efflux in response to the different NaCl and mannitol treatments. K+ flux showed different patterns bettwen NaCl and mannitol treatments, NaCl induced K+ efflux, however mannitol induced K+ efflux. Biphasic H+ flux was found under NaCl treatment, namely H+ flux was subjected to the changes from efflux to influx. Inhibitor experiment showed that the ATPdependent H+pump played a significant role in H+ efflux in response to salt stress.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：20101219修回日期：20110223 基金项目：国家自然科学基金 (30871727) 作者简介：高海波，男，博士研究生，研究方向为植物防御反应与信号转导。 *通讯作者：沈应柏，男，教授，博士生导师，研究方向为植物生理生态。Email：ybshen@bjfu.edu.cn。
更新日期/Last Update: 2011-12-31