[1]潘翠,钟留情,金朝美,等. 瑞香狼毒内生真菌降解宿主黄酮和香豆素类成分的研究[J].西北林学院学报,2013,28(02):150-155.[doi:10.3969/j.issn.1001-7461.2013.02.29]
　PAN Cui,ZHONG Liu-qing,JIN Zhao-mei,et al. Degradation of Host Flavonoids and Coumarins by Endophytic Fungi Isolated from Stellera chamaejasme[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(02):150-155.[doi:10.3969/j.issn.1001-7461.2013.02.29]
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 瑞香狼毒内生真菌降解宿主黄酮和香豆素类成分的研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第28卷
期数:
2013年02期
页码:
150-155
栏目:
出版日期:
2013-03-30

文章信息/Info

Title:
 Degradation of Host Flavonoids and Coumarins by Endophytic Fungi Isolated from Stellera chamaejasme
文章编号:
1001-7461(2013)02-0150-06
作者:
 潘翠; 钟留情; 金朝美; 宋晓平
 (西北农林科技大学 动物医学院，中兽医药研究所，陕西 杨陵 712100)
Author(s):
 PAN Cui; ZHONG Liu-qing; JIN Zhao-mei; SONG Xiao-ping
 (College of Veterinary Medicine,Institute of Traditional Chinese Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 ; 瑞香狼毒; 内生真菌; 降解
Keywords:
Stellera chamaejasme;  endophytic fungi;  degradation
分类号:
S713
DOI:
10.3969/j.issn.1001-7461.2013.02.29
文献标志码:
A
摘要:
 将瑞香狼毒内生真菌孢子悬液接种于以瑞香狼毒提取物为主要碳源的PDA培养基中，分别在培养的第0、3、5、7、9、12 天时，采用可见紫外分光光度法测定体系中黄酮和香豆素类成分含量，考察不同接种量（10%、20%、30%）、初始pH（5、6、7、8、9、10）和外加碳源（葡萄糖0、0.2%、0.5%、1.0%、1.5%、3.0%）对降解性能的影响。在培养第3天时，瑞香狼毒内生真菌C1Y74株对黄酮和香豆素类成分的降解作用明显，培养体系中残留量分别为432.24 mg/L和1.25 mg/L，极显著低于对照组474.68 mg/L和1.61 mg/L（p<0.01），在第12 天时达最低值，分别为303.78 mg/L和0.43 mg/L。在孢子接种量30%（V/V）、添加葡萄糖1%、初始pH=5时，体系中黄酮类化合物残留量为145.08 mg/L，极显著低于对照组568.27 mg/L（p<0.01），不添加葡萄糖时，体系中香豆素类化合物残留量为0.08 mg/L，极显著低于对照组0.61 mg/L（p<0.01）。
Abstract:
 The spore suspension of the endophytic fungi was incubated to the PDA culture medium with the addition of the extract of Stellera chamaejiasme as the main carbon source.The contents of residual flavonoids and coumarins in the cultural system were determined by ultraviolet-visible spectrophotometer,and the degradation effects of the initial inoculum size (10%,20%,30%), initial pH (5,6,7,8,9,10) and additional carbon source (glucose,0,0.2%,0.5%,1.0%,1.5%,3.0%) were investigated.It was found that at the 3rd day of the incubation,degradations of flavonoids and coumarins were obvious,and their contents in the residues were 432.24 mg/L and 1.25 mg/L,respectively,significantly lower than those in the control group: 474.68 mg/L and 1.61 mg/L (p<0.01).At the 12th day,the corresponding values were 303.78 mg/L and 0.43 mg/L，respectively.When the inoculating amount of spora was 30%（V/V）,the adding glucose was 1% and the initial pH was 5,the content of residual flavonoids in the system was 145.08 mg/L,significantly lower than that of the control group 568.27 mg/L，(p<0.01).When no adding glucose,the content of residual coumarins in the system was 0.08 mg/L,significantly lower than that of the control group 0.61 mg/L(p<0.01).

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备注/Memo

备注/Memo:
 收稿日期：2012-05-22 修回日期：2012-06-07
基金项目：“十一五”国家科技支撑计划资助硕目（2008BADB4B05）。
作者简介：潘翠，女，硕士，主要研究方向：中药活性成分。E-mail：panc-961@163.com
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