苹果砧木M<sub>9</sub>快繁技术的建立及试管苗生根进程解剖研究-《西北林学院学报》

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Title:: Anatomical Study on Rooting Process and Rapid Propagation of Apple Rootstock M₉

Author(s):: YU Liang; WANG Fei; （College of Horticulture, Northwest A&F University,Yangling,Shaanxi 712100,China）

Keywords:: apple dwarf rootstock M₉; in vitro shoot; suitable medium; rapid propagation system; adventitious root

摘要:: 通过对苹果砧木M₉离体培养，筛选该砧木的初代、继代培养、生根培养基，并解剖分析生根进程。结果表明：1）以M₉的离体新梢进行初代培养，75%酒精30 s+0.1%升汞8 min消毒效果较佳, 较适培养基为MS+6-BA1.0 mg·L^-1+NAA0.5 mg·L^-1。成活率为18.9%。2)M₉的适合增殖培养基MS+6-BA1.0 mg·L^-1+IBA0.1mg·L^-1, 有效新梢数达到6.4个·株^-1。3）较适生根培养基为1/ 2MS+ IBA0.3 mg·L^-1+NAA0.1 mg·L^-1。暗培养5 d后，光培养35 d，生根率达到85.5%。4）M₉试管苗不定根的发育过程可分为3个时期：形成层细胞分裂期，不定根原基形成期，不定根的分化形成期。

Abstract:: An anatomical observation was conducted on rooting process by in vitro culture of apple rootstock M₉.Related factors concerning the culture were examined.1)Satisfactory disinfection effects could be achieved with 75% alcohol for 30 s and 0.1% mercuric chloride for 8 min during the primary culture, and cultured with the medium of MS+6-BA 1.0 mg·L^-1+ NAA 0.5 mg·L^-1.The survival rate was 18.9%. 2) For M₉,the appropriate enrichment medium was MS+6-BA 1.0 mg·L^-1+IBA 0.1 mg·L^-1 in which the efficiency shoots was 6.4 per stem. 3) The proper rooting medium for M₉ was 1/ 2MS+IBA 0.3 mg·L^-1+NAA 0.1 mg·L^-1.Its rooting rate reached 85.5% when cultured in the dark for 5 days and light culture for 35 days.4)The process of M₉ plantlet adventitious root development could be divided into three periods:cell division of formation layer, the formation period of adventitious root primordia, differentiation stage of adventitious root.