[1]魏海霞,刘德玺,王 霞,等.2苹果品种叶片胚状体再生体系的建立[J].西北林学院学报,2014,29(01):84-88.[doi:10.3969/j.issn.1001-7461.2014.01.17]
　WEI Hai-xia,LIU De-xi,WANG Xia,et al.Establishment of Regeneration System of Embryoid from the Leaves of Two Apple Varieties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(01):84-88.[doi:10.3969/j.issn.1001-7461.2014.01.17]
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2苹果品种叶片胚状体再生体系的建立()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年01期
页码:
84-88
栏目:
林木遗传育种与培育
出版日期:
2014-01-21

文章信息/Info

Title:
Establishment of Regeneration System of Embryoid from the Leaves of Two Apple Varieties
文章编号:
1001-7461(2014)01-0084-05
作者:
魏海霞1; 刘德玺1; 王 霞1; 周 健1; 王莉莉1; 刘 丹2; 祝 军3*
1.山东省林业科学研究院,山东 济南 250014;
2.山东省林木种质资源中心,山东 济南 250014;
3.青岛农业大学,山东 青岛 266109
Author(s):
WEI Hai-xia1; LIU De-xi1; WANG Xia1; ZHOU Jian1; WAND Li-li1; LIU Dan2; ZHU Jun3*
1. Shandong Academy of Forestry, Ji’nan, Shandong 250014, China;
2. Shandong Forest Tree Germplasm Resources Center,Ji’nan, Shandong 250014, China;
3. Qingdao Agricultural University, Qingdao, Shandong 266109, China
关键词:
胚状体;  再生;  形态学;  组织学;  苹果
Keywords:
embryoid;  regeneration;  morphology;  histology;  apple
分类号:
S661.1
DOI:
10.3969/j.issn.1001-7461.2014.01.17
文献标志码:
A
摘要:
以苹果“富士”和“鲁加6号”为材料,建立叶片胚状体再生体系。结果表明:先经胚性细胞诱导培养基MS+BA1.0 mg·L-1+NAA5.0 mg·L-1+TDZ0.5 mg·L-1诱导胚性细胞,再置于胚胎发育培养基MS+BA1.0 mg·L-1+NAA0.1 mg·L-1上进行暗培养,最佳时间为10 d,2品种叶片再生率分别为96.7%和100%。经胚状体不同时期形态学组织学观察,显示胚状体发育时期经历原胚、球形胚、心形胚、子叶形胚、鱼雷形胚、成熟胚等阶段。“鲁加六号”苹果最佳生根培养基为1/2MS+IAA0.1 mg·L-1+IBA0.1 mg·L-1,生根率达86.7%。
Abstract:
Regeneration systems of 2 apple cultivars(Fuji and Lujia 6)were established by using leaf embryoids. First, embryonic cells were induced in the medium of MS+BA1.0 mg·L-1+NAA5.0 mg·L-1+TDZ0.5 mg·L-1,and then the resultant cells were cultured in the dark in MS+BA1.0 mg·L-1+NAA0.1 mg·L-1 with an optimal culture time of 10 d.The rates of leaf regeneration of 2 cultivars were 96.7% and 100%, respectively. Morphological and histological observations demonstrated that embryoids underwent different developmental periods: from original embryo to globular,cordiform,cotyledonary, fish torpedo-shaped, and mature embryos. The optimal rooting medium for ‘Lujia-6’ was obtained as 1/2MS+IAA0.1 mg·L-1+IBA0.1 mg·L-1,by which the rooting rate was 86.7%.

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相似文献/References:

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　XIAO Zheng,FAN Chong-hui,JIN Wan-mei.Effects of Plant Growth Regulators on Adventitious Shoot Regeneration of Dendranthema morifolium cv. Xiaojinhuang Leaf Discs[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2009,24(01):50.

备注/Memo

备注/Memo:
收稿日期:2013-06-20 修回日期:2013-09-16
基金项目:山东省自主创新专项资金“黄河三角洲盐碱地高效生态林业产业技术体系研究与示范”(2012CX90303); 山东省农业良种工程重大课题“林木种质资源收集保护与评价”(鲁农良字[2010]6号); 国家科技支撑计划主要果树新品种选育(2013BAD02B00); 中央财政农业技术推广项目; 山东省优秀中青年科学家奖励基金。
作者简介:魏海霞,女,工程师,研究方向:林木遗传育种、盐碱地改良。E-mail:weihaixia99@163.com
*通信作者:祝军,男,教授,博士,研究方向:果树生物技术。E-mail:junzhu@qau.edu.cn
更新日期/Last Update: 2014-01-20