[1]李玲莉,刘华敏,孔立生,等.利用生物反应器进行香水百合培养研究[J].西北林学院学报,2014,29(01):89-94.[doi:10.3969/j.issn.1001-7461.2014.01.18]
 LI Ling-li,LIU Hua-min,KONG Li-sheng,et al.Cultures of Perfume Lily in vitro with Airlift Bio-reactors[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(01):89-94.[doi:10.3969/j.issn.1001-7461.2014.01.18]
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利用生物反应器进行香水百合培养研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年01期
页码:
89-94
栏目:
林木遗传育种与培育
出版日期:
2014-01-21

文章信息/Info

Title:
Cultures of Perfume Lily in vitro with Airlift Bio-reactors
文章编号:
1001-7461(2014)01-0089-06
作者:
李玲莉1刘华敏1孔立生2韦冠吾1汤丽红1
1.重庆市风景园林科学研究院 城市园林绿化工程技术中心,重庆 401329;
2.维多利亚大学 森林生物学中心, 维多利亚市, 加拿大 V8W 3N5
Author(s):
LI Ling-li1 LIU Hua-min1 KONG Li-sheng2 WEI Guan-wu1 TANG Li-hong1
1. Urban Landscape Engineering Technology Research Center, Chongqing Landscape and Gardening Research Institute, Chongqing 401329, China;
2. Centre for Forest Biology, University of Victoria, Victoria, BC,Canada V8W 3N5
关键词:
香水百合 生物反应器 液体培养
Keywords:
perfume lily airlift bioreactor liquid culture
分类号:
S682.265
DOI:
10.3969/j.issn.1001-7461.2014.01.18
文献标志码:
A
摘要:
以香水百合的鳞片愈伤组织为试验对象进行生物反应器培养研究。结果表明:生物反应器在暗光条件下,气流量为20 mL·min-1时更有利于百合鳞片愈伤组织的正常生长,最佳诱导和增殖培养基为含有BA0.5 mg·L-1的MS培养基,最佳固体分化培养基为含有BA 0.5 mg·L-1和NAA0.5 mg·L-1的MS培养基。相对于固体培养而言,生物反应器增加了小仔球的分化能力; 避免不适气流量引起的组织褐化是影响培养物最佳生长发育的关键因子之一。
Abstract:
Calli were induced from bulb scales of perfume lily,and then cultured in airlift bio-reactors.The best growth of calli was achieved with air supplement of 20 mL·min-1 air-flow for these bioreactors in darkness.The optimum medium for both callus induction and bulking up was liquid MS medium supplemented with 0.5 mg·L-1 BA. Bulblets differentiated and grew after callus tissue was transferred onto semi-solid MS medium containing 0.5 mg·L-1 BA and 0.5 mg·L-1 NAA.Compared with semi-solid culture,bulblet differentiation was enhanced significantly in airlift bioreactors.It was essential to avoid tissue browning by using optimum air-flow rates for the best growth and development of plant tissue in the bioreactor.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-06-20 修回日期:2013-09-13
基金项目:重庆市科学技术委员会国际科技合作计划项目“利用生物反应器进行百合脱毒快繁研究”; 重庆市科技计划项目“科技平台与基地建设”(cstc2011pt-gc80019)。
作者简介:李玲莉,女,博士,高级园林工程师,研究方向:植物栽培及规模化育苗。E-mail:linglil009@163.com
更新日期/Last Update: 2014-01-20