[1]权金娥,朱海兰,张春霞,等. 四倍体刺槐茎段组织石蜡切片的制作方法[J].西北林学院学报,2014,29(03):140-144.[doi:doi:10.3969/j.issn.1001-7461.2014.03.28]
 QUAN Jin-e,ZHU Hai-lan,ZHANG Chun-xia,et al. Preparation of Paraffin Section of Tetraploid Robinia pseudoacacia Stem Segment Tissue[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(03):140-144.[doi:doi:10.3969/j.issn.1001-7461.2014.03.28]
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 四倍体刺槐茎段组织石蜡切片的制作方法()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年03期
页码:
140-144
栏目:
出版日期:
2014-05-30

文章信息/Info

Title:
 Preparation of Paraffin Section of Tetraploid Robinia pseudoacacia Stem Segment Tissue
文章编号:
1001-7461(2014)03-0140-05
作者:
 权金娥朱海兰张春霞张胜赵忠*
 (西北农林科技大学 西部环境与生态教育部重点实验室, 陕西 杨陵 712100)
Author(s):
 QUAN Jin-e ZHU Hai-lan ZHANG Chun-xia ZHANG Sheng ZHAO Zhong*
 (Key Laboratory of Environment and Ecology in Western China of Ministry of Education,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 四倍体刺槐林木茎段石蜡切片制作方法
Keywords:
 tetraploid Robinia pseudoacacia woody stem segment paraffin section preparation method
分类号:
S792.27
DOI:
doi:10.3969/j.issn.1001-7461.2014.03.28
文献标志码:
A
摘要:
 以传统石蜡切片方法为基础,针对林木茎段木质化程度高、硬度和脆性大的特点对固定、软化、脱水、透明、染色等具体步骤进行了改进,并成功应用于四倍体刺槐生根过程插穗基部解剖学研究。结果表明:较传统方法切片时组织不易碎,切片结构完整,减少了脱水时间,缩短了制片周期;获得了厚度为8 μm且染色清晰、细胞结构完整的林木茎段横切面切片;四倍体刺槐插穗石蜡切片可清晰的观察大量能够反映生根各时期发育特征。该方法为进一步从解剖学上研究林木茎段组织生长机制提供基础,为探究其组织形态变化提供了新的技术方法。
Abstract:
 Traditional method of making paraffin section was modified from the aspects of fixing,softening,dehydration,transparency,dyeing and other steps to fit the hard,crisp and highly lignified woody stem segment.This modified method was successfully applied to observe the rooting process of cuttings of Robinia pseudoacacia tetraploid.We made a 8 μm cross-section slice with clearly staining and complete organizational structure.The method reported in this study would provide theoretical and technical basis for anatomical observation to study the growing mechanism of woody plants as well as for identification of histomorphology changes.

参考文献/References:

 [1]李正理.植物制片技术[M].北京:科学出版社,1987.
[2]HENNIG G,GEHRMANN M,STROPP U,et al.Automated extraction of DNA and RNA from a single formalin-fixed paraffin-embedded tissue section for analysis of both single nucleotide polymorphisms and mRNA expression[J].Clinical Chemistry,2010,56(12):1845-1853.
[3]TAKAHASHI H,KAMAKURA H,SATO Y,et al.A method for obtaining high quality RNA from paraffin sections of plant tissues by laser microdissection[J].Journal of Plant Research,2010,123(6):807-813.
[4]朱耀军.牡丹茎扦插繁殖技术及生根机理初步研究[D].北京:中国林业科学研究院,2007.
[5]刘杏娥,汪佑宏,江泽慧,等.黄藤材发育过程中维管束的变化[J].西北林学院学报,2010,25(2):152-155.
LIU X E,WANG Y H,JIANG Z H,et al.Vascular bundle variation in the growth of Daemonorops margaritae[J].Journal of Northwest Forestry University,2010,25(2):152-155.(in Chinese)
[6]杨茂生,梅秀英,姜在民,等.黄帝陵侧柏叶受污染状况的形态解剖学研究[J].西北林学院学报,1994,9(3):12-16.
YANG M S,MEI X Y,JIANG Z M,et al.The morphological and anatomical study on the states of polluted seale-shaped leaves of the Platycladus orientalis in Huangdiling[J].Journal of Northwest Forestry University,1994,9(3):12-16.(in Chinese) 
[7]苏印泉,张军侠.10种茶树叶片比较解剖学及与抗性关系的研究[J].西北林学院学报,1998,13(4):1-8.
SU Y Q,ZHANG J X.A study on comparative anatomy and relationship with resistance of tea blades of 10 species[J].Journal of Northwest Forestry University,1998,13(4):1-8.(in Chinese) 
[8]RICCI A,ROLLI E,DRAMIS L,et al.N,N`-bis-(2,3-Methylenedioxyphenyl)urea and N,N`-bis-(3,4-methylenedioxyphenyl) urea enhance adventitious rooting in Pinus radiate and affect expression of genes induced during adventitious rooting in the presence of exogenous auxin[J].Plant Science,2008,175(4) 356-363.
[9]段昌盛,张守攻,王军辉,等.3种云杉嫩枝扦插生根特性的研究[J].西北林学院学报,2009,24(6):59-61.
DUAN C S,ZHANG S G,WANG J H,et al.Rooting capability of twigs of three spruce species[J].Journal of Northwest Forestry University,2009,24(6):59-61.(in Chinese)
[10]WANG X L,ZHAO Z,QUAN J E.Indole-3-butyric acid on rooting and endogenous plant hormones in tetraploid and diploid Robinia pseudoacacia hardwood cuttings[J].ΦYTON,2011,80:93-100.
[11]王小玲,赵忠,权金娥,等.外源激素对四倍体刺槐硬枝扦插生根及其关联酶活性的影响[J].西北植物学报,2011,31(6):116-122.
WANG X L,ZHAO Z,QUAN J E,et al.Rooting and correlative enzyme activities of hardwood cuttings of tetraploid Robinia pseudoacacia[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(6):116-122.(in Chinese)
[12]鲁丹,张瑞,彭方任,等.红桤木扦插繁殖技术及生根过程的解剖学观察[J].西南林业大学学报,2013,33(2):54-57.
LU D,ZHANG R,PENG F R,et al.Studies on cuttage propagation technique and anatomical observation on the rooting process of Alnus rubra cutting[J].Journal of Southwest Forestry University,2013,33(2):54-57.(in Chinese)
[13]马英,李明鹤,张新华,等.杉木嫩枝扦插不定根形成的解剖学研究[J].华中农业大学学报,1998,17(1):81-83.
MA Y,LI M H,ZHANG X H,et al.An anatomical study on the formation of adventitious roots of Chinese fir cutting[J].Journal of Huazhong Agricultural University,1998,17(1):81-83.(in Chinese)
[14]牛田,王厚新,李承秀,等.华北五角枫嫩枝扦插生根形态解剖学分析[J].山东林业科技,2012(5):19-21.
[15]王新刚.玫瑰扦插生根解剖学及繁殖技术研究[D].泰安:山东农业大学,2012.
[16]毛晓霞.石蜡切片制作方法的改良[J].安徽农学通报,2013,19(8)15-17.
[17]李素坤,张秋芝,郝玉兰,等.玉米成熟期茎秆石蜡切片方法的研究[J].安徽农业科学,2010,38 (8):3935-3937.
[18]BARBOSA A C F,PACE M R,WITOVISK L,et al.A new method to obtain good anatomical slides of heterogeneous plant parts[J].IAWA Journal,2010,31(4):373-383.
[19]姜维梅,张冬青,徐春霄.油菜茎的解剖结构和倒伏关系的研究[J].浙江大学学报:农业与生命科学版,2001,27(4):439-442. 
JIANG W M,ZHANG D Q,XU CH X.Studies on the stem anatomy of Brsssica oil to lodging[J].Journal of Zhejiang University:Agric.& life Sci.,2001,27 (4):439-442.(in Chinese)
[20]吴华,陈娉婷,袁玲,等.蕨类植物石蜡切片制作技术探讨[J].湖北农业科学,2011,50(18):3767-3769.

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备注/Memo

备注/Memo:
 收稿日期:2013-09-04 修回日期:2013-12-16
基金项目:国家自然科学基金项目“四倍体刺槐生根调控机理研究”(30972352)。
作者简介:权金娥,女,在读博士,研究方向:森林培育理论与技术。E-mail:quanjine@gmail.com
*通信作者:赵忠,男,教授,博士生导师,研究方向:森林培育理论与技术。E-mail: zhaozh@nswsuaf.edu.cn
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