[1]李薇,王永进,樊军锋*. 美洲黑杨新无性系的ISSR鉴别及SCAR标记转化[J].西北林学院学报,2014,29(04):98-102.[doi:doi:10.3969/j.issn.1001-7461.2014.04.17]
　LI Wei,WANG Yong-jin,FAN Jun-feng*. ISSR Identification and Transformation of SCAR Marker of Three New Clones of Populus deltoids[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(04):98-102.[doi:doi:10.3969/j.issn.1001-7461.2014.04.17]
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 美洲黑杨新无性系的ISSR鉴别及SCAR标记转化()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年04期
页码:
98-102
栏目:
出版日期:
2014-07-30

文章信息/Info

Title:
 ISSR Identification and Transformation of SCAR Marker of Three New Clones of Populus deltoids
文章编号:
1001-7461(2014)04-0098-05
作者:
 李薇1; 王永进2; 樊军锋1*
 (1.西北农林科技大学 林学院，陕西 杨陵 712100；2.岚皋县林业技术推广服务中心，陕西 岚皋 725400)
Author(s):
 LI Wei1; WANG Yong-jin2;  FAN Jun-feng1*
 （1. College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. Forestry Technology Popularization Service Center of Langao County, Langao, Shaanxi 725400, China)
关键词:
 ; SCAR; ISSR; 品种鉴定; 指纹图谱
Keywords:
 ; SCAR;  ISSR;  cultivar identification;  fingerprinting
分类号:
S792.119
DOI:
doi:10.3969/j.issn.1001-7461.2014.04.17
文献标志码:
A
摘要:
 为了从分子水平上对美洲黑杨新无性系材料进行准确快速鉴定，从100条ISSR引物中筛选出10条具多态性的引物对包括钻天杨和69杨在内的6个黑杨无性系开展指纹图谱的初步构建，并将钻天杨和03-北-1-15中扩增出的特异ISSR条带转为可用于快速鉴别黑杨无性系的SCAR标记。结果表明，ISSR指纹分析结合SCAR标记可以对供试黑杨无性系进行区分与鉴定，进而为杨树林木品种鉴定以及黑杨育种研究提供理论依据。
Abstract:
 In order to quickly and accurately identify the black poplar clones at the molecular level, we screened out 10 primers from 100 ISSR primers and preliminarily built the 6 clones’ fingerprint including 69 and Lombardy poplar. Two specific ISSR bands from 03-north 1-15 and Lombardy poplar were converted into two SCAR markers which were used to rapidly identify black poplar clones. Utilization of ISSR fingerprint and its SCAR marker for rapid identification of black poplar clones was practicable and significant, and the results provided theoretical basis for black poplar identification and breeding research.

参考文献/References:

 [1]徐立安，李新军，潘惠新，等. 用 SSR 研究栲树群体遗传结构[J]. 植物学报, 2001, 43(4): 409-412. 
XU L A ,LI X J, PAN H X, et al. Study on population genetic structure in Castanopsis fargesii with microsatelite markers[J]. Acta Botanica Sinica,2001,43(4):409-412. (in Chinese)
[2]李汝玉，宋国安，杨平平. 利用 SSR 标记进行品种权有争议品种的鉴定[J]. 种子, 2001(1): 66-67.
LL R Y,SONG G A,YANG P P, et al.Using SSR markers to identify the disputed varieties[J].Seed, 2001(1): 66-67. (in Chinese) 
[3]ZIETKIEWICZ E, RAFALSKI A, LABUDA D，et al. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J]. Genomics, 1994, 20(2): 176-183.
[4]GODWIN I D, AITKEN E A, SMITH L W, et al. Application of inter simple sequence repeat (ISSR) markers to plant genetics[J]. Electrophoresis, 1997, 18(9): 1524-1528.
[5]巩艳红, 刘军, 张健, 等. 毛豹皮樟的叶片DNA提取及其RAPD引物筛选[J]. 西北林学院学报，2004, 19(4):35-37.
GONG Y H, LIU J, ZHANG J, et al. DNA extraction and RAPD primer screening in leaves of Litsea coreana var. lanuginosa[J]. Jounml of Northwest Forestry University, 2004，19(4):35-37. (in Chinese)
[6]EDWARDS K, JOHNSTONE C, THOMPSON C，et al.A simple and rapid method for the preparation of plant genomic DNA for PCR analysis[J]. Nucleic Acids Research, 1991, 19(6): 1349.
[7]李绍臣. 东北主栽杨树品种的 ISSR 分析[M].哈尔滨: 东北林业大学出版社, 2004. 
[8]张志清，郑有良，魏育明，等. SSR 标记遗传距离与小麦杂种优势相关分析[J]. 四川农业大学学报, 2003, 21(1): 6-9. 
ZHANG Z Q, ZHENG Y L,WEI Y M, et al. Relationship between heterosis and genetic distance based on simple sequence repeat markers in wheat[J].Journal of Sichuan Agricultural University 2003,21(1):6-9. (in Chinese)
[9]MCGREGOR C, LAMBERT C, GREYLING M, et al. A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) [J]. germplasm. Euphytica, 2000, 113(2): 135-144.
[10]孔祥彬，张春庆，许子锋,等. DNA 指纹图谱技术在作物品种 (系) 鉴定与纯度分析中的应用[J]. 生物技术, 2005, 15(4): 74-77. 
KONG X B，ZHANG C Q,XU Z F, et al. The application of DNA fingerprinting technique used to identificate and purity analysis in crop varieties[J] . Biotechnology, 2005, 15(4): 74-77. (in Chinese) 
[11]汤玉喜，刘志祥，吴敏，等．XL-90等美洲黑杨杂交子代ISSR分子鉴别[J]. 中国农学通报，2011,27(02): 1-6. 
TANG Y X，LIU Z X，WU M，et al. Identification of hybrid progenies as Populus deltoides cl.’Xianglin 90’ etc. based on ISSR molecular markers[J]. Chinese Agricultural Science Bulletin, 2011, 27(02):1-6. (in Chinese)
[12]冯夏莲，何承忠，张志毅，等．毛白杨ISSR反应体系的建立及优化[J]．北京林业大学学报，2006，28(3): 6l-65．
FENG X L, HE C Z, ZHANG Z Y, et al. Establishment and optimization of an ISSR reaction system for Populus tomentosa Carr[J]. Journal of Beijing Forestry University, 2006, 28(3): 61-65. (in Chinese)
[13]GAO J M,ZHAN S G ,QI L W , et al.Application of ISSR marker to fingerprinting of elite cultvars (Varieties clones) from different section of the genus Populus L [J].Silvae Genetica,2001,55(1):1-4.
[14]ALBANI M C , BATTERY N H, WILKINSON M J. The devel-opment of ISSR-derived SCAR markers around the seasona lflowering locus (SFL) in Fragaria vesca[J].Theor Appl Genet., 2004, 109(3): 571-579.
[15]唐利华, 肖扬,边银丙, 等．中国黑木耳主要栽培菌株 ISSR 指纹分析及 SCAR 标记[J]．菌物学报, 2008, 27(2): 243-251.
TANG L H ,XIAO Y , BIAN Y B, et al. ISSR fingerprint analysis and SCAR marker of major cultivated strains of Auricularia auricula in China [J]. Mycosystema, 2008, 27(2): 243-251. (in Chinese) 
[16]孙保娟, 李植良,黎振兴,等．SCAR 标记转化失败的原因和对策[J]．分子植物育种, 2010, 8(3):589-594. 
SUN B J, LI Z L, LI Z X, et al. Reasons for scar conversion failure and its remedies[J]. Molecular Plant Breeding, 2010, 8(3): 589-594. (in Chinese)
[17]姜岳忠，李善文，秦光华，等. 黑杨派无性系区域化试验初报[J]. 林业科学, 2006, 42(12):143-147.
JIANG Y Z, LI S W, QIN G H, et al. Reginal test of poplar clones in section aigeiros[J].Scientia Silvae Sinicae ,2006,42(12):143-147. (in Chinese)
[18]张德强，张志毅，杨凯. 杨树分子标记研究进展[J]. 北京林业大学学报, 2000, 22(6): 79-84. 
ZHANG D Q, ZHANG Z Y, YANG K. Advances of molecular marker researches in poplar[J]. Journal of Beijing Forestry Uneiversity, 2000, 22(6): 79-84. (in Chinese)
[19]黎云昆. 植物新品种保护与种植业产业的发展[J]. 西北林学院学报, 2003, 18(1): 104-108.
LI Y K. Protection of new varieties and development of growing insustry [J]. Journal of Northwest Forestry University, 2003,18(1):104-108. (in Chinese)

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备注/Memo

备注/Memo:
 收稿日期：2013-11-07修回日期：2013-12-17
基金项目：国家“十二五”科技支撑专题“超高产优质杨树速生林新品种选育”(2012BAD01B03)。
作者简介：李薇，女，硕士研究生，研究方向：林木遗传育种。E-mail：lwzcc1987@163.com
*通信作者：樊军锋，男，教授，博士，研究方向：林木遗传育种及林业生物技术。E-mail: fanjf28@163.com
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