[1]吕世佳,张虹,虎娟,等. 康乃馨‘白雪公主’叶片培养植株再生[J].西北林学院学报,2014,29(06):114-118.[doi:doi:10.3969/j.issn.1001-7461.2014.06.22]
　LYU Shi-jia,ZHANG Hong,HU Juan,et al. Establishment of a Regeneration System for Dianthus caryophyllus Cultivar White Diana from Leaf Explant[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(06):114-118.[doi:doi:10.3969/j.issn.1001-7461.2014.06.22]
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 康乃馨‘白雪公主’叶片培养植株再生()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年06期
页码:
114-118
栏目:
出版日期:
2014-11-30

文章信息/Info

Title:
 Establishment of a Regeneration System for Dianthus caryophyllus Cultivar White Diana from Leaf Explant
文章编号:
1001-7461(2014)06-0114-05
作者:
 吕世佳1; 2; 张虹1; 2; 虎娟1; 2; 张芮1; 2; 王丽娟1; 2; 徐宏波3; 陈任1; 2*
 （1.宁夏大学 西部特色生物资源保护与利用教育部重点实验室，宁夏 银川 750021；2.宁夏大学 生命科学学院，宁夏 银川 750021；3.宁夏易林环境建设有限公司，宁夏 银川 750004）
Author(s):
 LYU Shi-jia1; 2;  ZHANG Hong1; 2;  HU Juan1; 2;  ZHANG Rui1; 2;  WANG Li-juan1; 2;  XU Hong-bo3;  CHEN Ren1; 2*
 (1. Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, Ningxia 750021, China; 2. School of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China; 3. Ningxia Yilin Enviroment Construction Co. Ltd. , Yinchuan, Ningxia 750004, China)
关键词:
 ; 康乃馨; 植物激素; 愈伤组织; 不定芽; 不定根; 再生
Keywords:
 ; carnation (Dianthus caryophyllus);  plant hormone;  callus inducement;  adventitious bud differentiation;  shoot elongation;  adventitious root differentiation
分类号:
S722.37
DOI:
doi:10.3969/j.issn.1001-7461.2014.06.22
文献标志码:
A
摘要:
 为建立一套快速、有效的康乃馨白色品种的再生体系，以康乃馨‘白雪公主’的幼叶为外植体，对其愈伤组织和不定芽诱导、不定芽伸长和不定根诱导各阶段所需的植物激素、植物材料的影响进行了探讨。结果表明，当叶片外植体的长度为5 mm时，在TDZ 0.5 mg·L-1、NAA 0.5 mg·L-1的MS培养基上，愈伤组织和不定芽的诱导效果最为理想；从愈伤组织切下不定芽，移植于单独添加NAA为0.5 mg·L-1的培养基能有效地促进不定芽的伸长；不定芽伸长至3~4 cm后，在添加NAA 0.1 mg·L-1的培养基上，不定根分化的数量最多；再生植株经移栽和炼苗后生长状态良好。
Abstract:
 In order to develop a rapid and efficient method for the regeneration of white carnation (Dianthus caryophyllus) cultivar, the leaf cuts from White Diana were used as explants, and the effects of plant hormones and materials on the stages of callus and adventitious bud inducement, shoot elongation, adventitious root inducement were determined. The results showed that the most calli and adventitious buds were induced from the leaf explants in the length of 5 mm on the MS basal medium supplied with 0.5 mg·L-1 TDZ combined with 0.5 mg·L-1 NAA. The adventitious buds which had been excised from the residual calli could be facilitatively elongated to shoots by transferring them to the medium with 0.5 mg·L-1 NAA alone. Shoots longer than 3~4 cm cultured on the medium supplied with 0.1 mg·L-1 NAA produced a maximum number of roots per shoot. Regenerated plantlets could be successfully acclimatized and transplanted to pods.

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备注/Memo

备注/Memo:
 收稿日期：2014-05-01修回日期：2014-08-19
基金项目：2013年度宁夏回族自治区科技支撑计划项目（413-0396）。
作者简介：吕世佳，女，在读硕士，研究方向：代谢植物学。E-mail: lv_shijia@163.com
*通信作者：陈任，男，教授，博士，研究方向：植物分子生物学与基因工程。E-mail: chenren@nxu.edu.cn
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