[1]吴媛琳,赵听,张凯煜,等. 枇杷不同部位主要有效成分含量及抗氧化活性比较[J].西北林学院学报,2015,30(01):196-201.[doi:doi:10.3969/j.issn.1001-7461.2015.01.32]
 WU Yuan-lin,ZHAO Ting,ZHANG Kai-yu,et al. Main Active Ingredient Contents and the Antioxidant Activity of Different Parts of Eriobotrya japonica[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(01):196-201.[doi:doi:10.3969/j.issn.1001-7461.2015.01.32]
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 枇杷不同部位主要有效成分含量及抗氧化活性比较()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年01期
页码:
196-201
栏目:
出版日期:
2015-01-31

文章信息/Info

Title:
 Main Active Ingredient Contents and the Antioxidant Activity of Different Parts of Eriobotrya japonica
文章编号:
1001-7461(2015)01-0196-06
作者:
 吴媛琳1赵听1张凯煜1张社奇1*刘建军2康永祥2
 (1.西北农林科技大学 理学院,陕西 杨陵 712100;2.西北农林科技大学 林学院,陕西 杨陵 712100)
Author(s):
 WU Yuan-lin1 ZHAO Ting1 ZHANG Kai-yu1 ZHANG She-qi1* LIU Jian-jun2 KANG Yong-xiang2
 (1. College of Sciences, Northwest A&F University, Yangling, Shanxi 712100,China; 2. College of Forestry, Northwest A&F University, Yangling, Shanxi 712100, China)
关键词:
 枇杷总黄酮总酚酸总三萜酸抗氧化活性
Keywords:
 Eriobotrya japonica total flavonoid total phenolic acid tritepenoidic acid antioxidant activity
分类号:
S713
DOI:
doi:10.3969/j.issn.1001-7461.2015.01.32
文献标志码:
A
摘要:
 以枇杷不同部位(花蕾、花、老叶、新叶、枝条、花梗)为试验材料,采用分光光度法测定枇杷不同部位总黄酮、总酚酸、总三萜酸含量,并以Vc为标准,选用DPPH·和FRAP法对不同部位醇提取物进行抗氧化活性评价,研究枇杷不同部位总黄酮、总酚酸、总三萜酸的分布情况以及抗氧化活性。结果表明,枇杷各部位3种主要有效成分均存在显著差异,花蕾总黄酮、总酚酸、总三萜酸含量显著高于其他部位,分别为196.01、68.32、96.28 mg·g-1,约是含量最低的新叶的3.16倍、1.96倍、2.12倍;在抗氧化活性方面,枇杷花蕾的醇提取物的抗氧化活性最强,是新叶的2.5倍以上,经相关性分析发现,抗氧化活性与总黄酮和总酚酸含量均具有较高相关性,其中与总黄酮的相关性最大。枇杷各部位中,花蕾的抗氧化活性最强,各主要有效成分含量也最高,枇杷花蕾有望被开发为新的抗氧化原料。
Abstract:
 Plant tissues from different parts of Eriobotrya japonica (bud, flower, old leaf, new leaf, branch, and flower peduncle) were collected to measure the contents of flavonoids, phenolic acid, and triterpenoid acid. Antioxidant activities of the alcoholic extracts from different parts were measured. Significant differences were found in the contents of three ingredients among different parts of E. japonica. Higher contents of three ingredients were observed in bud, 68.32 mg·g-1, 196.01 mg·g-1, 96.28 mg·g-1 for flavonoids, total phenolic acid and total triterpenoid acid, respectively, 3.16, 1.96, and 2.12 times higher than those found in new leaves in which the contents of the three ingredient were the lowest. In term of antioxidant activity, the alcohol extract of the buds was the strongest that was 2.5 times more than the new leaves. High correlationship was observed between the antioxidant activity and the content of total flavonoids and total phenolic acids. It was concluded that the bud of E. japonica exhibited the highest antioxidant activity, and the highest contents of ingredient, which is expected to be developed into a new antioxidant material.

参考文献/References:

 [1]王立为, 刘新民, 余世春, 等.枇杷叶抗炎和止咳作用研究[J]. 中草药,2004, 35(2): 174-176.
WANG L W, LIU X M, YU S C, et al. Anti-inflammatory and antitussive effects of Eriobotrya japonica [J]. Chinese Traditional and Herbal Drugs, 2004, 35(2): 174-176.(in Chinese)
[2]HUANG Y, LI J , CAO Q, et al. Anti-oxidative effect of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. leaf in chronic bronchitis rats [J]. Life Sciences, 2006,78(23): 2749-2757.
[3]郭宇, 吴松吉, 朴惠善. 枇杷叶的化学成分及药理活性研究进展[J]. 时珍国医国药, 2006,17(6): 928-930.
[4]王静波,杨必坤,张宏,等. 枇杷花提取物止咳作用研究[J]. 中草药,2009,40(7):1106-1109.
[5]何莲, 张宏, 李琪, 等. 枇杷花系统溶剂提取物抑菌作用研究[J].食品科学, 2007,28(12): 109-112.
[6]DUFOUR D, PICHETTE A, MSHYILDADAE V, et al. Antioxidant, anti-inflammatory and anticancer activities of methanolic extracts from Ledum groenlandicum Retzius[J]. Journal of Ethnopharmacology, 2007, 111:22-28.
[7]YANG J, MEYERS K J, HEIDE J V D, et al. Varietal differences in phenolic content and antioxidant and antiproliferative activities of onions[J]. Journal Agricultural Food Chemistry, 2004, 52(22):6787-6793.
[8]李琨,张学杰,张德纯,等. 不同芹菜品种叶与叶柄黄酮含量及其与抗氧化能力的关系[J]. 园艺学报,2011,38(1):69-76.
LI K, ZHANG X J, ZHANG D C, et al. The quantitation of flavonoids in leaf and stalk of different celery cultivars and the correlation with antioxidation activity [J]. Acta Horticulturae Sinica, 2011,38(1):69-76. (in Chinese)
[9]龚晓武,李炳奇,刘丹丹,等. 红景天黄酮提取及其抗氧化活性研究[J]. 西北林学院学报,2011,26(3):136-138.
GONG X W, LI B Q, LIU D D, et al. Antioxidant activity of the flavonoid extract from rhodiola rosea[J]. Journal of Northwest Forestry University, 2011, 26(03):136-138. (in Chinese)
[10]黄福山,袁卫明,俞文生. 枇杷大棚栽培疏花疏果技术[J]. 江西园艺,2003(6):10.
[11]罗锋,汪河滨,杨玲,等. 超声——微波协同萃取法提取甘草黄酮的研究[J]. 食品研究与开发,2006,27(8):127-128.
[12]吴媛琳,王世军,张社奇,等. 修剪对苹果枝(梢)皮层总黄酮含量的影响[J]. 西北林学院学报,2013,28(6):103-107.
WU Y L, WANG S J, ZHANG S Q, et al. Effect of puring on the content of total flavonoids in the branch bark of apple trees[J]. Journal of Northwest Forestry University,2013,28(6):103-107.
[13]韩菊,魏福祥. Folin-Ciocalteu比色法测定苹果渣中的多酚[J]. 食品科学,2010,31(4):179-182.
[14]周巧霞,张经硕,顾明,等. 测定山楂及提取物总三萜酸的含量[J]. 中国野生植物资源, 2004,23(5): 43-44.
[15]张雁冰,王克让,刘宏民. 马桑叶中总三萜酸的含量测定[J]. 时珍国医国药,2006,17(4):529-530.
[16]KIM D O,LEE K W,LEE H J, et al.Vitamin C equivalent antioxidant capacity(VCEAC) of phenolic phytochemicals[J]. Journal Agricultural Food Chemistry,2002,50:3713-3717.
[17]林恋竹,赵谋明. 反应时间对DPPH·法、ABTS+·法评价抗氧化性结果的影响[J]. 食品科学,2010,31(05):63-67.
[18]LUO W, ZHAO M M, YANG B, et al. Identification of bioactive compounds in Phyllenthus emblica L. fruit and their free radical scavenging activities[J]. Food Chemistry, 2009, 114: 499-504.
[19]石雪萍,吴亮亮,高鹏,等. 20种食用辛香料抗氧化性及其与黄酮和多酚的相关性研究[J]. 食品科学,2011,32(5):83-86.
SHI X P, WU L L, GAO P, et al. Ethanol extracts from twenty edible spices: antioxidant activity and its correlations with total flavonoids and total phenols content [J]. Food Science, 2010, 32(5):83-86. (in Chinese)
[20]赵则海,于景华,杨逢建,等. 人为扰动对乌拉尔甘草不同部位甘草酸与总黄酮含量的影响[J]. 生态学报,2004,24(12):2799-2803.
ZHAO Z H, YU J H, YANG F J, et al. Influences of artificial disturbance degrees on the contents of Glycyrrhizic acid and flavonoids in different parts of Glycyrrhiza uralensis[J]. Acta Ecologica Sinica, 2004, 24(12):2799-2803. (in Chinese)
[21]杨蓓芬,金则新,邵红,等. 七子花不同器官次生代谢产物含量的分析[J]. 植物研究,2007,27(2):229-232.

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备注/Memo

备注/Memo:
 收稿日期:2014-03-23 修回日期:2014-05-21
基金项目:“十二五”农村领域国家科技计划课题(2011BAD38B0603);林业公益性行业科研专项(200904004)。
作者简介:吴媛琳,女,硕士研究生,研究方向:植物生理与植物资源利用。E-mail:wyl281305571@163.com 
*通信作者:张社奇,男,教授,研究方向: 环境生物物理与土壤生态。E-mail: zhangsheqi@nwsuaf.edu.cn
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