[1]杨颜沁,廖珍,伍景艳,等. 基于木屑发酵的蛋白酶活性分析[J].西北林学院学报,2015,30(01):219-221.[doi:doi:10.3969/j.issn.1001-7461.2015.01.37]
　YANG Yan-qin,LIAO Zhen,WU Jing-yan,et al. Protease Activity Analysis Based on Sawdust Fermentation[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(01):219-221.[doi:doi:10.3969/j.issn.1001-7461.2015.01.37]
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 基于木屑发酵的蛋白酶活性分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年01期
页码:
219-221
栏目:
出版日期:
2015-01-31

文章信息/Info

Title:
 Protease Activity Analysis Based on Sawdust Fermentation
文章编号:
1001-7461(2015)01-0219-03
作者:
 杨颜沁; 廖珍; 伍景艳; 李昭; 董宁文; 朱晓冬; 刘玉*
 (东北林业大学 材料科学与工程学院，黑龙江 哈尔滨 150040)
Author(s):
 YANG Yan-qin; LIAO Zhen;  WU Jing-yan;  LI Zhao;  DONG Ning-wen;  ZHU Xiao-dong; LIU Yu*
 （College of Material Science and Engineering, Northeast Forestry University, Harbin, Heilongjiang 150040, China）
关键词:
 ; 木屑; 发酵; 蛋白酶; 活性分析
Keywords:
 ; sawdust; fermentation;  protease;  activity analysis
DOI:
doi:10.3969/j.issn.1001-7461.2015.01.37
文献标志码:
A
摘要:
 木屑是木材使用过程中的废弃品，但是在一定的环境条件下发酵可产生有益于人体的蛋白酶。采用枯草芽孢杆菌、水果酵素与桧木醇作为发酵菌进行核桃楸木屑发酵试验，通过对比不同发酵方法产生蛋白酶的活性，研究适合木屑的发酵方法。结果表明，不同发酵菌作用下核桃楸木屑发酵后蛋白酶的活性都有所增加，其中在加入枯草芽孢杆菌发酵剂的同时还分别加入等量的水果酵素和桧木醇可以有效提高蛋白酶的活性，比未发酵蛋白酶的活性提高了8.1％。本项目的研究工作可以更高效地利用木屑资源，使木材资源的作用不仅仅局限于材料层面而进一步的扩展至人体生物学层面。
Abstract:
 Sawdust is the waste products in the process of wood. But in certain conditions of fermentation，it can produce protease beneficial to human beings. Bacillus subtilis, fruit enzymes and hinokitol were used as fermentation of Juglans mandshurica sawdust. The protease activities were compared between different fermentation methods. The results showed that the protease activity all increased after the addition of different bacterial during fermentation. In which joined addition of the equal amounts of fruit enzymes and hinokitol with B. subtilis can effectively improve the activity of protease by 8.1%. The research in this paper can use wood resources more efficiently, and make the use from the material aspect to the human biology level.

参考文献/References:

 [1]徐勇,焦杰,徐斌伟,等.杨木屑低浓度硫酸中温预处理提取木糖及其发酵[J]. 林产化学与工业,2011,31(5):11-17.
XU Y,JIAO J,XU B W, et al. Extraction and fermentation of xylose from poplar sawdust by hot sulfuric acid pretreatment at low concentration[J]. Chemistry and Industty of Forest Products,2011,31(5):11-17.(in Chinese)
[2]付雯,张晓勇,周金燕,等. 固定化枯草芽孢杆菌发酵生产捷安肽素[J]. 应用与环境生物学报,2009,15(2):230-234.
FU W,ZHANG X Y,ZHOU J Y, et al. Jiean-peptide prodution by immobilized cell fermentation of bacillus subtilis[J]. Chinese Journal of Applied & Environmental Biology,2009,15(2):230-234.(in Chinese)
[3]刘建伟,卢伟东,贾培培,等. 松木屑不同处理方法对绣球菌生长的影响[J]. 中国食用菌,2010,29(1):70-71.
[4]徐勇,顾依娜,范丽,等. 杨木稀酸预处理液木糖发酵产乙醇工艺条件的研究[J]. 林产化学与工业,2010,30(3):19-23.
XU Y,GU Y N,FAN L, et al. Study on the processing conditions of xylose fermentation to produce fuel ethanol from dilute-acid pretreated poplar[J]. Chemistry and Industty of Forest Products, 2010,30(3):19-23.(in Chinese)
[5]谢小林,顾振红,陈美标,等. 潮湿纤维单胞菌和绿色木霉混合接种对不同食用菌菌渣相关酶活性的影响[J]. 生物技术通报,2013（12）:162-166.
XIE X L, GU Z H, CHEN M B, et al. Study on the related enzyme activities in the residues of different mushroom substrates inoculated with Cellulomonas uda and Trichoderma viride[J].Biotechnology Bulletin,2013(12):162-166.(in Chinese)
[6]艾斌凌. 纤维素酶的液体发酵与杨木生物转化乙醇[D].长沙:中南林业科技大学,2009.
[7]徐璐,文连奎. 固定化醋酸菌发酵人参醋饮料加工工艺优化[J]. 食品工业科技,2012,33(24):311-314.
XU L, WEN L K. Optimization of immobilization of acetic acid bacteria for ginseng vinegar drinks[J]. Science and Technology of Food Industry,2012,33(24):311-314.(in Chinese)
[8]周如宝. 用木屑碱熔法制草酸[J]. 适用技术之窗,1994(6):10.
[9]杜甫佑. 白腐菌木质纤维素降解次序研究[D].武汉:华中科技大学,2004.
[10]宋卡魏. 枯草芽孢杆菌B68发酵培养的优化及两种剂型的初步研究[D].广州:华南热带农业大学,2007.
[11]龚黛,郭蔚,王华,等. 葡萄枝条栽培白玉菇的营养成分研究[J]. 西北林学院学报,2013,28（6）:121-124.
GONG D,GUO W,WANG H, et al. Nutrition components in white Hypsizygus marmoreus cultivated with vine pruning stalks[J]. Journal of Northwest Forestry University,2013,28（6）:121-124.(in Chinese)
[12]邹勇,尉芹,赵忠,等. 木屑快速热解生物油中酚类提取物的抗氧化及抑真菌活性研究[J]. 西北林学院学报,2014,29（1）:116-121.
ZOU Y,WEI Q,ZHAO Z, et al. Antioxidant and antimicrobial activities of phenolic extract from bio-oil by fast pyrolysis of sawdust[J]. Journal of Northwest Forestry University, 2014,29（1）:116-121.(in Chinese)
[13]ELISASHVILI V, PARLAR H, KACHLISHVILI E. Ligninolytic activity of basidiomycetes grown under submerged and solid-state fermentation on plant raw material (sawdust of grapevine cuttings) [J]. Advances in Food Sciences, 2001，23(3), 117-123.
[14]IMMANUEL G, BHAGAVATH C, RAJ P I. Production and partial purification of cellulase by aspergillus niger and A. fumigatus fermented in coir waste and sawdust[J]. Internet Journal of Microbiology, 2007，3(1)：101-113
[15]OJUMU T V, SOLOMON B O, BETIKU E. Cellulase production by aspergillus flavus linn isolate NSPR 101 fermented in sawdust, bagasse and corncob[J]. African Journal of Biotechnology, 2003，2(6):150-152.

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备注/Memo

备注/Memo:
 收稿日期：2014-03-27 修回日期：2014-04-23
基金项目：东北林业大学大学生创新训练项目(201310225133)。
作者简介：杨颜沁，女，研究方向：生物质材料利用。E-mail: 524266923@qq.com
*通信作者：刘玉，女，讲师，博士，研究方向：生物质复合材料。E-mail: liuyu820524@126.com
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